Collagen IV and laminin expression in squamous cell carcinomas of lower lip and tongue

Objective: In this study, the expression of the extracellular matrix proteins was immunohistochemically studied and compared with the histological grading of squamous cell carcinomas of the lower lip and tongue. Material and Methods: The lower lip carcinomas (n=12) and the tongue carcinomas (n=12) were histopathologically graduated according to Bryne’s method. The immunohistochemical technique utilized specific antibodies to collagen IV and laminin. Histopathologic and immunohistochemical analysis were carried-out on the tumoral invasive front. Results: Most of lower lip carcinomas (91.7%) was classified in lower score and all tongue carcinomas (100%) in high score malignant grade (p<0.01). Collagen type IV expression was absent in the peritumoral basement membrane in 50% of lower lip carcinomas and in 66.7% of tongue carcinomas (p=0.09). Laminin expression was absent in the peritumoral basement membrane in 66.7% of lower lip carcinomas and in 58.3% of tongue carcinomas (p=0.48). When these two glicoproteins were expressed, they showed a linear, thin and discontinuous pattern and a weak intensity of expression. Conclusion: The high score malignancy grade of the tongue carcinomas associated with the expression pattern of the studied matrix proteins. It suggests that tongue squamous cell carcinomas have more invasive potential and more aggressive biological behavior than the lower lip carcinomas. KeywordsCollagen type IV; Laminin; Carcinoma; Immunohistochemistry.

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Type IV collagen immunoreactivity of basement membrane in inflammatory-regenerative and dysplastic lesions of the flat colonic mucosa

Bosnian Journal of Basic Medical Sciences ◽

10.17305/bjbms.2003.3567 ◽

2003 ◽

Vol 3 (1) ◽

pp. 30-35

Author(s):

Svjetlana Radović ◽

Ivan Selak ◽

Mirsad Babić ◽

Željka Knežević ◽

Zora Vukobrat-Bijedić

Keyword(s):

Basement Membrane ◽

Colon Adenocarcinoma ◽

Normal Mucosa ◽

Collagen Iv ◽

Epithelial Dysplasia ◽

Collagen Type Iv ◽

Severe Dysplasia ◽

Colon Mucosa ◽

Mild Dysplasia ◽

Type Iv

The aim of this research is to establish by immunohistochemistry if there is a change in the expression of collagen type IV, as a substitute of basement membrane, in development of epithelial dysplasia in chronically inflamed colon mucosa.Methods. Biopsy specimens from 270 patients were examined: 74 were classified as inflammatory-regenerative and 196 as dysplastic lesions. There were 108 cases of mild dysplasia, 58 cases of moderate and 30 cases severe dysplasia, respectively. Visualisation of collagen IV and its way of expression within basement membrane of glandular crypts was performed by immunohistochemistry and then compared with findings in normal colon mucosa and colon adenocarcinoma tissue.Results. Changes in the expression of collagen IV comprised of its focal irregularities, diffuse thinning and/or thickening, focal interruptions or its complete absence. Significant changes in the expression of collagen IV in relation to normal mucosa already occur in inflammatory-regenerative mucosa. In mild dysplasia, these changes are more intensive in relation to those in inflammatory altered mucosa as well as at severe dysplasia in relation to moderate dysplasia. Changes in the expression of collagen IV in severe dysplasia are significantly more serious than in moderate dysplasia but are identical to those in colon adenocarcinoma tissue.Conclusion. These findings suggest that change in the expression of collagen IV is in correlation to a degree of epithelial dysplasia that developed in flat chronically inflamed colon mucosa.

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Immunohistochemical analysis of FoxP3+ regulatory T cells in lower lip squamous cell carcinomas

Brazilian Oral Research ◽

10.1590/1807-3107bor-2016.vol30.0130 ◽

2016 ◽

Vol 30 (1) ◽

Cited By ~ 2

Author(s):

Fernando Antonio Portela da CUNHA FILHO ◽

Maria Cássia Ferreira de AGUIAR ◽

Lélia Batista de SOUZA ◽

Leão PEREIRA PINTO ◽

Gustavo Pina GODOY ◽

...

Keyword(s):

T Cells ◽

Regulatory T Cells ◽

Squamous Cell ◽

Immunohistochemical Analysis ◽

Squamous Cell Carcinomas ◽

Lower Lip

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Localization of Collagen Type IV Alpha Chains at the Basement Membrane of Oral Squamous Cell Carcinoma

Journal of Hard Tissue Biology ◽

10.2485/jhtb.14.194 ◽

2005 ◽

Vol 14 (2) ◽

pp. 194-195

Author(s):

Ryo Tamamura ◽

Hitoshi Nagatsuka ◽

Mehmet Gunduz ◽

Hong Gong Liu ◽

Noriyuki Nagai

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Basement Membrane ◽

Cell Carcinoma ◽

Squamous Cell ◽

Collagen Type ◽

Collagen Type Iv ◽

Type Iv

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Interaction of Plasmodium gallinaceum ookinetes and oocysts with extracellular matrix proteins

Parasitology ◽

10.1017/s0031182099004874 ◽

1999 ◽

Vol 119 (4) ◽

pp. 331-336 ◽

Cited By ~ 39

Author(s):

A. ADINI ◽

A. WARBURG

Keyword(s):

Extracellular Matrix ◽

Basement Membrane ◽

Specific Antigen ◽

Growth Factor Receptor ◽

Extracellular Matrix Proteins ◽

Growth Period ◽

Collagen Iv ◽

Matrix Proteins ◽

Collagen Type Iv ◽

Plasmodium Gallinaceum

Plasmodium ookinetes are elongate, motile and invasive while inside the mosquito gut but promptly metamorphose into spherical immobile oocysts upon coming in contact with the basement membrane surrounding the midgut. There they begin a prolonged growth period characterized by massive DNA synthesis for the production of sporozoites. Living Plasmodium gallinaceum ookinetes attached avidly to the murine extracellular matrix proteins, laminin and collagen type IV. In ELISA-type assays, the main ookinete surface protein, Pgs28 was implicated as a mediator of parasite attachment to these basement membrane constituents. Laminin and collagen IV adhered to ookinete and oocyst lysates spotted onto nitrocellulose membranes. Receptor–ligand blot assays demonstrated that Pgs28 and an oocyst-specific antigen recognized by the mAb 10D6 interact with murine collagen IV and laminin. 10D6 antigen was also recognized by monospecific antiserum against the human epidermal growth factor receptor. Mosquito-derived laminin was incorporated into oocyst capsules of P. gallinaceum growing in Aedes aegypti. We hypothesize that contact with the mosquito basement membrane triggers the transformation of ookinetes into oocysts. Coalescence of basement membrane proteins onto the capsules masks developing oocysts from the mosquito's immune system and facilitates their prolonged extracellular development in the mosquito body cavity.

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Immunohistochemical expression of E-cadherin and CD44v6 in squamous cell carcinomas of the lower lip and tongue

Brazilian Dental Journal ◽

10.1590/s0103-64402009000100011 ◽

2009 ◽

Vol 20 (1) ◽

pp. 64-69 ◽

Cited By ~ 9

Author(s):

Maria Carmen Fontoura Nogueira da Cruz ◽

Antonio Luiz Amaral Pereira ◽

Fernanda Ferreira Lopes ◽

Cassiano Francisco Weege Nonaka ◽

Raimunda Ribeiro da Silva ◽

...

Keyword(s):

Squamous Cell ◽

Nodal Metastasis ◽

Squamous Cell Carcinomas ◽

Biological Behavior ◽

Anatomical Location ◽

Histological Grading ◽

Immunohistochemical Expression ◽

Lower Lip ◽

Significant Difference ◽

E Cadherin

This study analyzed the immunohistochemical expression of E-cadherin and CD44v6 in 15 squamous cell carcinomas (SCCs) of lower lip and 15 SCCs of tongue in order to verify a possible association between these proteins and the anatomic location of the lesion, nodal metastasis and histological grading of malignancy. The pattern of expression and number of immunopositive cells were evaluated. The results were analyzed with the Fisher's exact test, Mann-Whitney test and Spearman's Correlation Coefficient (r). using the SPSS software 10.0 for Windows. Statistical significance was set at 5% determined for a p-value<0.05 for all tests. There was no significant difference (p>0.05) in the pattern of expression and number of immunopositive cells for E-cadherin and CD44v6, regarding the anatomical location and nodal metastasis. For the histological grading, low score SCCs showed higher immunopositivity for E-cadherin and CD44v6, both for the pattern of expression and number of immunopositive cells (p<0.05). There was a negative correlation between the total score of malignancy and the pattern of expression and number of immunopositive cells for E-cadherin and CD44v6 (p<0.05). In conclusion, SCCs of the lower lip and tongue did not reveal significant differences in the expression of E-cadherin and CD44v6. The expression of these adhesion molecules revealed association only with tumor histological grading of malignancy. Therefore, these results suggest that E-cadherin and CD44v6 may not help elucidating the differences between the biological behavior of SCCs of the lower lip and tongue.

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CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1

The Journal of Cell Biology ◽

10.1083/jcb.200602132 ◽

2006 ◽

Vol 175 (4) ◽

pp. 563-569 ◽

Cited By ~ 70

Author(s):

Mizuho Fukunaga-Kalabis ◽

Gabriela Martinez ◽

Zhao-Jun Liu ◽

Jiri Kalabis ◽

Paul Mrass ◽

...

Keyword(s):

Basement Membrane ◽

Basal Layer ◽

Spatial Localization ◽

Collagen Iv ◽

Collagen Type Iv ◽

Matricellular Protein ◽

Adhesion Receptor ◽

Type Iv ◽

Epidermal Homeostasis ◽

Discoidin Domain Receptor 1

Melanocytes reside within the basal layer of the human epidermis, where they attach to the basement membrane and replicate at a rate proportionate to that of keratinocytes, maintaining a lifelong stable ratio. In this study, we report that coculturing melanocytes with keratinocytes up-regulated CCN3, a matricellular protein that we subsequently found to be critical for the spatial localization of melanocytes to the basement membrane. CCN3 knockdown cells were dissociated either upward to the suprabasal layers of the epidermis or downward into the dermis. The overexpression of CCN3 increased adhesion to collagen type IV, the major component of the basement membrane. As the receptor responsible for CCN3-mediated melanocyte localization, we identified discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase that acts as a collagen IV adhesion receptor. DDR1 knockdown decreased melanocyte adhesion to collagen IV and shifted melanocyte localization in a manner similar to CCN3 knockdown. These results demonstrate an intricate and necessary communication between keratinocytes and melanocytes in maintaining normal epidermal homeostasis.

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An Immunohistochemical Analysis of Keratoacanthomas and Squamous Cell Carcinomas.

Nishi Nihon Hifuka ◽

10.2336/nishinihonhifu.58.625 ◽

1996 ◽

Vol 58 (4) ◽

pp. 625-628

Author(s):

Yoshiko EGAMI ◽

Toshihiko MASHINO ◽

Shuhei IMAYAMA ◽

Yoshiaki HORI

Keyword(s):

Squamous Cell ◽

Immunohistochemical Analysis ◽

Squamous Cell Carcinomas

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RECONSTRUCTION OF THE LOWER LIP AFTER EXCISION OF SQUAMOUS CELL CARCINOMAS

10.26226/morressier.578e3b50d462b802923826f4 ◽

2016 ◽

Author(s):

manuel acosta

Keyword(s):

Squamous Cell ◽

Squamous Cell Carcinomas ◽

Lower Lip

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Faculty Opinions recommendation of Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1064801.517728 ◽

2007 ◽

Author(s):

Taina Pihlajaniemi

Keyword(s):

Basement Membrane ◽

Collagen Type ◽

Collagen Type Iv ◽

Type Iv ◽

Basement Membrane Collagen ◽

Membrane Collagen ◽

Embryonic Death

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Serum Anti-Collagen IV IgM and IgG Antibodies as Indicators of Low Vascular Turnover of Collagen IV in Patients with Long-Term Complications of Type 2 Diabetes

Diagnostics ◽

10.3390/diagnostics11050900 ◽

2021 ◽

Vol 11 (5) ◽

pp. 900

Author(s):

Krasimir Kostov ◽

Alexander Blazhev

Keyword(s):

Type 2 Diabetes ◽

Serum Levels ◽

Vascular Wall ◽

Collagen Iv ◽

Collagen Type Iv ◽

Non Invasive ◽

Type Iv ◽

Antibody Levels

Thickening of the vascular basement membrane (BM) is a fundamental structural change in the small blood vessels in diabetes. Collagen type IV (CIV) is a major component of the BMs, and monitoring the turnover of this protein in type 2 diabetes (T2D) can provide important information about the mechanisms of vascular damage. The aim of the study was through the use of non-invasive biomarkers of CIV (autoantibodies, derivative peptides, and immune complexes) to investigate vascular turnover of CIV in patients with long-term complications of T2D. We measured serum levels of these biomarkers in 59 T2D patients with micro- and/or macrovascular complications and 20 healthy controls using an ELISA. Matrix metalloproteinases-2 and -9 (MMP-2 and MMP-9) were also tested. In the T2D group, significantly lower levels of CIV markers and significantly higher levels of MMP-2 and MMP-9 were found compared to controls. A significant positive correlation was found between IgM antibody levels against CIV and MMP-2. These findings suggest that vascular metabolism of CIV is decreased in T2D with long-term complications and show that a positive linear relationship exists between MMP-2 levels and CIV turnover in the vascular wall.

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