Enzyme-Linked Immunosorbent Assay Method for Human Autophosphorylated Insulin Receptor: Applicability to Insulin-Resistant States

The ability of Pediococcus pentosaceus to inhibit Clostridium botulinum toxigenesis in minimally heat-treated, vacuum-packaged sous vide-type beef with gravy was investigated. The bacteriocinogenic strain P. pentosaceus ATCC 43200 and the nonbacteriocinogenic strain P. pentosaceus 43NP1 were coinoculated with proteolytic and nonproteolytic C. botulinum types A and B spores into minimally processed meat with gravy. Toxin was present in samples inoculated with C. botulinum alone by day 31 at 4°C and by day 6 at 10°C. When coinoculated with C. botulinum, neither strain of Pediococcus was capable of significantly delaying the appearance of toxin. Although an enzyme-linked immunosorbent assay method for botulinal toxin was useful for screening toxin-positive samples, a high proportion of false negatives was revealed by confirmatory mouse bioassays. This research confirms that, if botulinal spores are present, sous vide beef does present a botulinal hazard, even when kept under adequate refrigeration.

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Detection of virus causes papaya ringspot virus - with the DAS-Elisa (Double Antibody Sandwich-Enzyme-Linked Immunosorbent Assay) method at different levels in North Sumatra

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/454/1/012182 ◽

2020 ◽

Vol 454 ◽

pp. 012182

Author(s):

L Hartati ◽

D Bakti ◽

A R Tantawi ◽

Lisnawita

Keyword(s):

Immunosorbent Assay ◽

Ringspot Virus ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Papaya Ringspot Virus ◽

Double Antibody ◽

North Sumatra ◽

Different Levels ◽

Das Elisa

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Fecal Cortisol and Progesterone Concentrations in Post Partus of Etawah Crossbreed Goat

E3S Web of Conferences ◽

10.1051/e3sconf/202015101031 ◽

2020 ◽

Vol 151 ◽

pp. 01031

Author(s):

Claude M. Airin ◽

Amelia Hana ◽

Sarmin Sarmin ◽

Pudji Astuti

Keyword(s):

Immunosorbent Assay ◽

Assay Method ◽

Enzyme Linked Immunosorbent Assay ◽

Stress Hormone ◽

Fecal Samples ◽

Fecal Cortisol ◽

Fecal Extract ◽

Last Stage ◽

Preliminary Study ◽

Freeze Dryer

Progesterone (P4) is a dominant hormone during pregnancy. In the later stage of pregnancy, the stress hormone particularly cortisol (C) may increase for initiating the parturition process as a consequence of fetal stress. This study was a preliminary study to compare the concentration of P4 and C in feces of Etawah Crossbreed Goat during their last stage of pregnancy and post partus. This study used 5 pregnant Etawah Crossbreed Goats (t 20th weeks) of pregnancy. Fecal samples were collected in the 20th week of pregnancy to 2 weeks of postpartum. All fecal samples were then dried using a freeze dryer (Labfreez FD10-MR) for 7 days at -80°C. Afterward, dried feces were pulverized and extracted by using 3ml of methanol 80%. The fecal extract was then analyzed the P4 and C concentrations using the enzyme-linked immunosorbent assay method. Concentrations of P4 and C metabolites in the last stage of pregnancy were 5,506.18 3,396.72 ng/g dry feces and 136,625.83 42,479.22 ng/g feces, respectively. Concentrations of P4 and C metabolites in the 2 weeks postpartum decreased at 669.38 P 643.9 ng/g feces and 110,295 / 14,378, 8 ng/g feces, respectively. It canbe concluded that there was a difference in the fecal progesterone and cortisol concentrations between the last phase of pregnancy and the postpartum phase.

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