Efficient gene transfer to dispersed human pancreatic islet cells in vitro using adenovirus-polylysine/DNA complexes or polycationic liposomes

Diabetes ◽  
1996 ◽  
Vol 45 (9) ◽  
pp. 1197-1203 ◽  
Author(s):  
J. Saldeen ◽  
D. T. Curiel ◽  
D. L. Eizirik ◽  
A. Andersson ◽  
E. Strandell ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Dna Complexes ◽  
Human Pancreatic Islet ◽  
Efficient Gene

Efficient Gene Transfer to Dispersed Human Pancreatic Islet Cells In Vitro Using Adenovirus-Polylysine/DNA Complexes or Polycationic Liposomes

Diabetes ◽  
1996 ◽  
Vol 45 (9) ◽  
pp. 1197-1203 ◽  
Author(s):  
J. Saldeen ◽  
D. T. Curiel ◽  
D. L. Eizirik ◽  
A. Andersson ◽  
E. Strandell ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Dna Complexes ◽  
Human Pancreatic Islet ◽  
Efficient Gene

Cytotoxic T-lymphocyte–mediated killing of human pancreatic islet cells in vitro

2008 ◽  
Vol 69 (9) ◽  
pp. 543-551 ◽  
Author(s):  
Peter D. Campbell ◽  
Eugene Estella ◽  
Nadine L. Dudek ◽  
Gaurang Jhala ◽  
Helen E. Thomas ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
T Lymphocyte ◽  
Cytotoxic T Lymphocyte ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Human Pancreatic Islet

Modulation of Specific Beta Cell Gene (Re)Expression during In Vitro Expansion of Human Pancreatic Islet Cells

2003 ◽  
Vol 12 (7) ◽  
pp. 799-807 ◽  
Author(s):  
Thomas Bouckenooghe ◽  
Brigitte Vandewalle ◽  
Bruno Lukowiak ◽  
Julie Kerr-Conte ◽  
Sandrine Beläich ◽  
...  
Keyword(s):  
Beta Cell ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Human Pancreatic Islet ◽  
In Vitro Expansion ◽  
Cell Gene

scholarly journals Improved Baculovirus Vectors for Transduction and Gene Expression in Human Pancreatic Islet Cells

Viruses ◽  
2018 ◽  
Vol 10 (10) ◽  
pp. 574 ◽  
Author(s):  
Leo Graves ◽  
Mine Aksular ◽  
Riyadh Alakeely ◽  
Daniel Ruiz Buck ◽  
Adam Chambers ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Islet Transplantation ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Pancreatic Islet Transplantation ◽  
Post Transplantation ◽  
Safe Alternative ◽  
Human Pancreatic Islet

Pancreatic islet transplantation is a promising treatment for type 1 diabetes mellitus offering improved glycaemic control by restoring insulin production. Improved human pancreatic islet isolation has led to higher islet transplantation success. However, as many as 50% of islets are lost after transplantation due to immune responses and cellular injury, gene therapy presents a novel strategy to protect pancreatic islets for improved survival post-transplantation. To date, most of the vectors used in clinical trials and gene therapy studies have been derived from mammalian viruses such as adeno-associated or retrovirus. However, baculovirus BacMam vectors provide an attractive and safe alternative. Here, a novel BacMam was constructed containing a frameshift mutation within fp25, which results in virus stocks with higher infectious titres. This improved in vitro transduction when compared to control BacMams. Additionally, incorporating a truncated vesicular stomatitis virus G protein increased transduction efficacy and production of EGFP and BCL2 in human kidney (HK-2) and pancreatic islet β cells (EndoC βH3). Lastly, we have shown that our optimized BacMam vector can deliver and express egfp in intact pancreatic islet cells from human cadaveric donors. These results confirm that BacMam vectors are a viable choice for providing delivery of transgenes to pancreatic islet cells.

Dietary fatty acids modify insulin secretion of rat pancreatic islet cells in vitro

2002 ◽  
Vol 25 (5) ◽  
pp. 436-441 ◽  
Author(s):  
F. J. Tinahones ◽  
A. Pareja ◽  
F. J. Soriguer ◽  
J. M. Gómez-Zumaquero ◽  
F. Cardona ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Insulin Secretion ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Dietary Fatty Acids ◽  
Pancreatic Islet Cells ◽  
Rat Pancreatic Islet

Cytotoxic Effects of Cytokines on Human Pancreatic Islet Cells in Monolayer Culture*

1990 ◽  
Vol 71 (1) ◽  
pp. 152-156 ◽  
Author(s):  
ALEXANDER RABINOVITCH ◽  
WILMA SUMOSKI ◽  
RAYMOND V. RAJOTTE ◽  
GARTH L. WARNOCK
Keyword(s):  
Pancreatic Islet ◽  
Monolayer Culture ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Cytotoxic Effects ◽  
Human Pancreatic Islet

Galectin-3 deficiency protects pancreatic islet cells from cytokine-triggered apoptosis in vitro

2013 ◽  
Vol 228 (7) ◽  
pp. 1568-1576 ◽  
Author(s):  
Tamara Saksida ◽  
Ivana Nikolic ◽  
Milica Vujicic ◽  
Ulf J. Nilsson ◽  
Hakon Leffler ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Galectin 3
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