scholarly journals High total serum renin concentrations are associated with the development of background retinopathy in adolescents with type 1 diabetes

Diabetes Care ◽  
2000 ◽  
Vol 23 (7) ◽  
pp. 1025-1026 ◽  
Author(s):  
O. Kordonouri ◽  
A. Wladimirowa ◽  
T. Danne
Keyword(s):  
Type 1 Diabetes ◽  
Total Serum ◽  
Background Retinopathy

scholarly journals How the sialylation level of serum N-acetyl-β-D-glucosaminidase a form in type 1 diabetes mellitus influences its activity?

2014 ◽  
Vol 79 (12) ◽  
pp. 1491-1503
Author(s):  
Vesna Jovanovic ◽  
Jelena Acimovic ◽  
Vesna Dimitrijevic-Sreckovic ◽  
Ljuba Mandic
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Structural Changes ◽  
Negative Charge ◽  
Total Serum ◽  
Secondary Complications ◽  
Isoenzyme Patterns ◽  
Healthy Persons

It has been verified that serum N-acetyl-?-D-glucosaminidase (NAG) activity is elevated in diabetes, but there are no reports about changes of the sialic acid (SA) content in the carbohydrate parts of NAG A form and its influence on total NAG activity changes in type 1 diabetes mellitus patients without and with secondary complications. NAG A forms were isolated, purified and characterized from the serum of 81 IDDM patients with and without secondary complications (retinopathy, polyneuropathy and nephropathy) and 25 healthy persons. The content of ?-2,6-bound SA and isoenzyme patterns of purified A form, total NAG and A form activities were determined. In all diabetic groups, A form sialylation levels were 2-3.5 times lower compared to control, while their acidities (fractions with pI 4.25-5.1) increased, particularly with progression of secondary complications. Total serum NAG activities and percentages of A form were significantly higher (P<0.001) in all diabetic groups and negatively correlated with the ?-2,6-bound SA content of the A form. In addition, they decreased as secondary diabetic complications became more complex. Observed changes could be the consequence of structural changes in the A form due to significant increase in its acidity, i.e. negative charge which originate from groups other than SA.

scholarly journals Immunological Functions of Oxidized Human Immunoglobulin G in Type 1 Diabetes Mellitus: Its Potential Role in Diabetic Smokers as a Biomarker of Elevated Oxidative Stress

2011 ◽  
Vol 31 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Zafar Rasheed ◽  
Hani A. Al-Shobaili ◽  
Abdullateef A. Alzolibani ◽  
Muhammad Ismail Khan ◽  
Muhammad Tariq Ayub ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 1 Diabetes ◽  
Immunoglobulin G ◽  
Competitive Inhibition ◽  
Human Immunoglobulin ◽  
Total Serum Protein ◽  
Total Serum ◽  
Human Immunoglobulin G ◽  
Direct Binding

The role of oxidized immunoglobulin G in type 1 diabetic smokers has been investigated in the present study. Human immunoglobulin G (IgG) was modified by reactive oxygen species (ROS). The binding characteristics of circulating autoantibodies in type 1 diabetes patients against native and modified IgG were assessed by direct binding ELISA. High degree of specific binding by 68.5% of patients sera towards ROS-modified IgG was observed in comparison to its native analogue (p< 0.05). In addition, diabetic smokers (n= 28) were examined and the results were compared with diabetic non-smokers (n= 26). Circulating antibodies of diabetic smokers showed substantially stronger binding to modified IgG as compared with the antibodies present in diabetic non-smokers (p< 0.05). Normal human sera (n= 53) showed negligible binding with either antigen. Competitive inhibition ELISA reiterates the direct binding results. The increase in total serum protein carbonyl levels in the diabetic smokers was largely due to an increase in oxidized IgG. Diabetic smokers showed substantially higher carbonyl contents in sera as well as in purified IgG as compared with sera and IgG of diabetic non-smokers. Collectively, the oxidation of plasma proteins, especially IgG, might enhance oxidative stress in type 1 diabetes smokers.

scholarly journals Diabetic background retinopathy is associated with impaired coronary vasoreactivity in people with Type 1 diabetes

Diabetologia ◽  
2004 ◽  
Vol 47 (4) ◽  
pp. 725-731 ◽  
Author(s):  
J. Sundell ◽  
T. Janatuinen ◽  
T. R�nnemaa ◽  
O. T. Raitakari ◽  
J. Toikka ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Background Retinopathy
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