Analysis of the Transcriptome of Group A Streptococcus in Mouse Soft Tissue Infection

ABSTRACTObtaining essential nutrients, such as carbohydrates, is an important process for bacterial pathogens to successfully colonize host tissues. The phosphoenolpyruvate phosphotransferase system (PTS) is the primary mechanism by which bacteria transport sugars and sense the carbon state of the cell. The group A streptococcus (GAS) is a fastidious microorganism that has adapted to a variety of niches in the human body to elicit a wide array of diseases. A ΔptsImutant (enzyme I [EI] deficient) generated in three different strains of M1T1 GAS was unable to grow on multiple carbon sources (PTS and non-PTS). Complementation withptsIexpressed under its native promoter in single copy was able to rescue the growth defect of the mutant. In a mouse model of GAS soft tissue infection, all ΔptsImutants exhibited a significantly larger and more severe ulcerative lesion than mice infected with the wild type. Increased transcript levels ofsagAand streptolysin S (SLS) activity during exponential-phase growth was observed. We hypothesized that early onset of SLS activity would correlate with the severity of the lesions induced by the ΔptsImutant. In fact, infection of mice with a ΔptsI sagBdouble mutant resulted in a lesion comparable to that of either the wild type or asagBmutant alone. Therefore, a functional PTS is not required for subcutaneous skin infection in mice; however, it does play a role in coordinating virulence factor expression and disease progression.

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Genome-wide discovery of novel M1T1 group A streptococcal determinants important for fitness and virulence during soft-tissue infection

PLoS Pathogens ◽

10.1371/journal.ppat.1006584 ◽

2017 ◽

Vol 13 (8) ◽

pp. e1006584 ◽

Cited By ~ 27

Author(s):

Yoann Le Breton ◽

Ashton T. Belew ◽

Jeffrey A. Freiberg ◽

Ganesh S. Sundar ◽

Emrul Islam ◽

...

Keyword(s):

Soft Tissue ◽

Soft Tissue Infection ◽

Tissue Infection ◽

Genome Wide ◽

Group A

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Invasive group A streptococcal soft tissue infection and the streptococcal toxic shock like syndrome

Plastic Surgery ◽

10.4172/plastic-surgery.1000098 ◽

1995 ◽

Vol 3 (1) ◽

Author(s):

Frankie OG Fraulin ◽

Martin J Giuffre

Keyword(s):

Soft Tissue ◽

Soft Tissue Infection ◽

Tissue Infection ◽

Toxic Shock ◽

Invasive Group ◽

Group A

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Necrotizing Fasciitis of The Abdominal Wall Following Colonoscopy with Fatal Outcome - A Case Report

Surgical Case Reports ◽

10.31487/j.scr.2019.05.06 ◽

2019 ◽

pp. 1-3

Author(s):

Daniel Matz ◽

Oleg Heizmann

Keyword(s):

Soft Tissue ◽

Necrotizing Fasciitis ◽

Soft Tissue Infection ◽

Group A Streptococcus ◽

Subcutaneous Tissue ◽

Fatal Outcome ◽

Anaerobic Bacteria ◽

Tissue Infection ◽

Life Threatening ◽

Staphylococcus Spp

Necrotizing fasciitis (NF) is a serious and potentially life threatening soft tissue infection, usually caused by different types of bacteria such as group A streptococcus, staphylococcus spp. (type 1 infection) or mixed infection by aerobic and anaerobic bacteria (type 2 infection). Usually, the infection arises from skin injury, in injections or surgical procedures and effects the fascia as well as the subcutaneous tissue. Overwhelming progression and difficulties in diagnosing are very common. Mortality rate is up to 100% depending on the type of soft tissue infection and did not markedly decrease in the past decades [1]. Here we present a case of NF with fatal outcome following colonoscopy, which was primarily suspected to be a post polypectomy syndrome.

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Phosphotransferase System Uptake and Metabolism of the β-Glucoside Salicin Impact Group A Streptococcal Bloodstream Survival and Soft Tissue Infection

Infection and Immunity ◽

10.1128/iai.00346-20 ◽

2020 ◽

Vol 88 (10) ◽

Cited By ~ 1

Author(s):

Rezia Era Braza ◽

Aliyah B. Silver ◽

Ganesh S. Sundar ◽

Sarah E. Davis ◽

Afrooz Razi ◽

...

Keyword(s):

Soft Tissue ◽

Soft Tissue Infection ◽

Tissue Infection ◽

Phosphotransferase System ◽

Content Type ◽

Growth And Survival ◽

Successful Infection ◽

Group A ◽

The Individual

ABSTRACT Streptococcus pyogenes (group A Streptococcus [GAS]), a major human-specific pathogen, relies on efficient nutrient acquisition for successful infection within its host. The phosphotransferase system (PTS) couples the import of carbohydrates with their phosphorylation prior to metabolism and has been linked to GAS pathogenesis. In a screen of an insertional mutant library of all 14 annotated PTS permease (EIIC) genes in MGAS5005, the annotated β-glucoside PTS transporter (bglP) was found to be crucial for GAS growth and survival in human blood and was validated in another M1T1 GAS strain, 5448. In 5448, bglP was shown to be in an operon with a putative phospho-β-glucosidase (bglB) downstream and a predicted antiterminator (licT) upstream. Using defined nonpolar mutants of the β-glucoside permease (bglP) and β-glucosidase enzyme (bglB) in 5448, we showed that bglB, not bglP, was important for growth in blood. Furthermore, transcription of the licT-blgPB operon was found to be repressed by glucose and induced by the β-glucoside salicin as the sole carbon source. Investigation of the individual bglP and bglB mutants determined that they influence in vitro growth in the β-glucoside salicin; however, only bglP was necessary for growth in other non-β-glucoside PTS sugars, such as fructose and mannose. Additionally, loss of BglP and BglB suggests that they are important for the regulation of virulence-related genes that control biofilm formation, streptolysin S (SLS)-mediated hemolysis, and localized ulcerative lesion progression during subcutaneous infections in mice. Thus, our results indicate that the β-glucoside PTS transports salicin and its metabolism can differentially influence GAS pathophysiology during soft tissue infection.

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