scholarly journals EVALUASI MASTITIS SUBKLINIS PADA SAPI PERAH DI PETERNAKAN RAKYAT KAPUPATEN TANGGAMUS MENGGUNAKAN UJI MASTITIS DAN JUMLAH SEL SOMATIK

Author(s):  
Dian Cahya Hadi ◽  
Veronica Wanniatie ◽  
Arif Qisthon ◽  
Purnama Edy Santosa

This study aimed to determine the presence or absence of subclinical mastitis cases with mastitis test IPB-1 and somatic cell count calculation of the Breed method for dairy milk farm in Tanggamus Regency.  The study was conducted in January 2020. Milk specimens were taken from farmers in Gisting Atas and Air Naningan district areas of Tanggamus Regency, Lampung Province.  Specimen testing was carried out at the Animal Production Laboratory, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung.  Specimens of milk used were obtained from farms in the Gisting Atas region as many as 9 lactation dairy cows and 10 lactation dairy cows from farms in the Air Naningan region.  This research method was a survey by taking farm data through interviews, taking milk specimens for mastitis testing with the reagents of IPB-1, taking specimens to be used as preparations which are then tested to somatic cell count using the breed method.  The variables observed were the results of mastitis test and the results of somatic cell counts in dairy milk.  Data were processed in tabulation and histogram then explained descriptively.  The mastitis test results showed that 33% of cow nipples from Gisting Atas farms and 37% of nipples from Air Naningan farms positively had subclinical mastitis.  Calculation of the number of somatic cells showed that the average number of somatic cells was 2,341,538 cells / ml for the Gisting Atas area and as much as 821,176 cells / ml for the Air Naningan region.    Keywords: Subclinical mastitis, IPB-1 test, Breed method, Somatic cells, dairy milk

2015 ◽  
Vol 18 (4) ◽  
pp. 799-805 ◽  
Author(s):  
A. Bortolami ◽  
E. Fiore ◽  
M. Gianesella ◽  
M. Corrò ◽  
S. Catania ◽  
...  

Abstract Subclinical mastitis in dairy cows is a big economic loss for farmers. The monitoring of subclinical mastitis is usually performed through Somatic Cell Count (SCC) in farm but there is the need of new diagnostic systems able to quickly identify cows affected by subclinical infections of the udder. The aim of this study was to evaluate the potential application of thermographic imaging compared to SCC and bacteriological culture for infection detection in cow affected by subclinical mastitis and possibly to discriminate between different pathogens. In this study we evaluated the udder health status of 98 Holstein Friesian dairy cows with high SCC in 4 farms. From each cow a sample of milk was collected from all the functional quarters and submitted to bacteriological culture, SCC and Mycoplasma spp. culture. A thermographic image was taken from each functional udder quarter and nipple. Pearson’s correlations and Analysis of Variance were performed in order to evaluate the different diagnostic techniques. The most frequent pathogen isolated was Staphylococcus aureus followed by Coagulase Negative Staphylococci (CNS), Streptococcus uberis, Streptococcus agalactiae and others. The Somatic Cell Score (SCS) was able to discriminate (p<0.05) cows positive for a pathogen from cows negative at the bacteriological culture except for cows with infection caused by CNS. Infrared thermography was correlated to SCS (p<0.05) but was not able to discriminate between positive and negative cows. Thermographic imaging seems to be promising in evaluating the inflammation status of cows affected by subclinical mastitis but seems to have a poor diagnostic value.


2009 ◽  
Vol 76 (3) ◽  
pp. 326-330 ◽  
Author(s):  
Olga Wellnitz ◽  
Marcus G Doherr ◽  
Marta Woloszyn ◽  
Rupert M Bruckmaier

Determination of somatic cell count (SCC) is used worldwide in dairy practice to describe the hygienic status of the milk and the udder health of cows. When SCC is tested on a quarter level to detect single quarters with high SCC levels of cows for practical reasons, mostly foremilk samples after prestimulation (i.e. cleaning of the udder) are used. However, SCC is usually different in different milk fractions. Therefore, the goal of this study was the investigation of the use of foremilk samples for the estimation of total quarter SCC. A total of 378 milkings in 19 dairy cows were performed with a special milking device to drain quarter milk separately. Foremilk samples were taken after udder stimulation and before cluster attachment. SCC was measured in foremilk samples and in total quarter milk. Total quarter milk SCC could not be predicted precisely from foremilk SCC measurements. At relatively high foremilk SCC levels (>300×103 cells/ml) foremilk SCC were higher than total quarter milk. At around (50–300)×103 cells/ml foremilk and total quarter SCC did not differ considerably. Most interestingly, if foremilk SCC was lower than 50×103 cells/ml the total quarter SCC was higher than foremilk SCC. In addition, individual cows showed dramatic variations in foremilk SCC that were not very well related to total quarter milk SCC. In conclusion, foremilk samples are useful to detect high quarter milk SCC to recognize possibly infected quarters, only if precise cell counts are not required. However, foremilk samples can be deceptive if very low cell numbers are to be detected.


2012 ◽  
Vol 5 (1) ◽  
pp. 59-68
Author(s):  
Ivana Davidov ◽  
Miodrag Radinović ◽  
Mihajlo Erdeljan ◽  
Ivan Stančić ◽  
Dragica Stojanović ◽  
...  

The study was conducted on 15 Holstein-Friesian cows aged between 3 and 5 years kept in the free grazing conditions in summer. The samples of milk were taken twice a year for monitoring subclinical mastitis. In addition to milk samples, the blood samples were taken to determine the concentration of zinc in serum. The occurrence of subclinical mastitis in high yielding cows was estimated based on the values of zinc concentration in blood serum and somatic cells of milk. By analyzing the samples of blood serum, a variation in the concentration of zinc in blood serum was noted depending on the season. Somatic cell count in the spring and autumn period in average was over 400.000/ml milk. Based on statistical analysis, the correlation test, it was concluded that the concentration of zinc in blood serum of cows in the experimental group had no significant effect on the somatic cells count in milk.


2002 ◽  
Vol 45 (4) ◽  
pp. 349-357
Author(s):  
Z. Baltay

Abstract. This research indicates that the numerical value of the emptying of somatic cells as a result of the effect of time of day cannot be demonstrated and interval difference less than 2 hours between milking does not significantly influence the formation of somatic cells and their absolute quantity. Moreover it was confirmed that subclinical mastitis also occurs in individual udder quarters of healthy qualified cows producing bulk milk with somatic cell count below 400 thousand / ml. Udder quarter with mastitis was found in 15,2 % of cows of the tested stock realising this production, which can be considered a significant proportion and is a justification for eliminating and taking udder health care of these animals


Author(s):  
E. Mohammedi ◽  
A. Sword ◽  
D. J. Roberts

Somatic cell count of milk is a well established test of monitoring udder subclinical mastitis in dairy cattle. Its diurnal variation has been known for decades, (SMITH and SHULTZE 1967). However the association of somatic cell count to time of sampling has been poorly investigated (AOKI et al 1990), and little emphasis has been given to the representativity of samples for a period of 24h. By concentrating on the study of differences between morning and evening milkings throughout lactation, the objective of the current study was to stress the need for a standard representative sampling time, to avoid possible bias in the interpretation of somatic cell count data, whether for research purposes or routine health surveys.


2013 ◽  
Vol 29 (4) ◽  
pp. 659-664
Author(s):  
I. Davidov ◽  
M. Radinovic ◽  
M. Erdeljan ◽  
Z. Kovacevic ◽  
Z. Jurakic ◽  
...  

The objective of this study was to evaluate the influence of blood and milk zinc concentration on somatic cell count, milk production and occurrence of subclinical mastitis cases. The study was performed on thirty Holstein cows approximate same body weight, ages 3 to 5 years, with equally milk production. Blood samples were taken after the morning milking from the caudal vein and milk from all four quarters was taken during morning milking. All samples of blood and milk were taken to determined zinc, using inductively coupled plasma mass spectrometry. 37.67% (11/30) cows have blood serum zinc concentration below 7?mol/l, and 63.33% or 19/30 cows have blood serum zinc concentration higher then 13?mol/l. Also 30% (9/30) cows have somatic cell count lower then 400.000/ml which indicate absence of subclinical mastitis, but 70% (21/30) cows have somatic cell count higher then 400.000/ml which indicate subclinical mastitis. According to all results in this research, zinc has very important influence on reducing subclinical mastitis and somatic cell count in milk of dairy cows.


1976 ◽  
Vol 39 (4) ◽  
pp. 250-252
Author(s):  
D. M. MACAULAY ◽  
R. E. GINN ◽  
V. S. PACKARD

A Coulter Counter, Model ZBI, was introduced into a provincial government laboratory and adapted for use in counting somatic cells in milk. The procedure followed was a modification of the International Dairy Federation method, the main difference being in the formulation of the fixative and a slight change in diluent make-up. The procedure was further modified in operating details to enhance routine analytical work. During one year of use in the ongoing provincial laboratory program, 8130 milk samples were checked for somatic cells by the Coulter Counter. Of this number, 509 were selected at random and checked by the Direct Microscopic Somatic Cell Count (DMSCC) method. The correlation coefficient for these samples for the two methods was 0.973. The mean cell counts were 841.600 and 839,900 for the DMSCC and Coulter Counter methods, respectively. The regression formula for estimating DMSCC from Coulter Counter results was determined to be ŷ= 0.8023(x) + 163.45.


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