DEVELOPMENT OF NEOLAMARCKIA CADAMBA (KELEMPAYAN) TISSUE CULTURE TECHNIQUES FOR SUSTAINABLE SUPPLY OF PLANTING MATERIALS FOR COMMERCIAL PLANTATION

2015 ◽  
Vol 77 (24) ◽  
Author(s):  
Siti Suhaila A. Rahman ◽  
Norwati Muhammad ◽  
Nor Hasnida Hassan ◽  
Haliza Ismail ◽  
Nazirah Abdullah ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Mass Production ◽  
Nodal Segments ◽  
Direct Organogenesis ◽  
Timber Species ◽  
Culture Techniques ◽  
Commercial Plantation ◽  
Planting Materials ◽  
Tissue Culture Techniques

Neolamarckia cadamba (kelempayan) is a multipurpose and fast growing timber species. The tree is grown for timber, paper-making and as ornamental plant. It is reported that its barks and leaves possesed medicinal values and its flowers are used in perfumes. The species is also known to be suitable for plywood, packing case, toys and short-fibred pulp. Therefore, mass production of high quality planting material of N. cadamba is important to support plantation program of this species. Here we presented mass production of N. cadamba through tissue culture techniques. Nodal segments derived from in vitro germinated seeds were used and induced direct organogenesis to produce shoots and roots using MS media (1962) and plant growth regulators (BAP and IBA) that are relatively cheaper than previously used methods. The tissue culture technique of N. cadamba developed may help in ensuring supply of planting materials that are feasible for commercial plantation purposes.

scholarly journals Plant Tissue culture and Ultra High Diluted studies: suggesting a novel model using in vitro techniques

2021 ◽  
Vol 15 (4) ◽  
pp. 45-49
Author(s):  
Carolina Santos Barreto ◽  
Fortune Homsani ◽  
Carla Holandino ◽  
Nina Claudia Barboza Da Silva
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Length ◽  
Nodal Segments ◽  
Culture Vessel ◽  
Culture Techniques ◽  
Lippia Alba ◽  
Tissue Culture Techniques

Plant tissue culture techniques have been used to evaluate the effects of many different substances and/ or conditions in plant growth and development. It provides information of great value about problems related to basic and applied aspects of plant as well as contributed to understanding of factors responsible for growth, metabolism, synthesis of secondary compounds, stress response. Considering all this wide range of applications and as all plant tissue culture techniques are undergone under axenic and controlled conditions (culture medium composition, light and temperature, for instance), it seems to be a value model for Ultra High Diluted (UHD) studies. Lippia alba is a Brazilian plant that tissue cultures protocols and in vitro essential oil production have already been described in scientific literature. None of all scientific papers evaluated the effects of UHD substances on in vitro development or secondary metabolic production. The main goal was to evaluate the use of plant tissue culture to investigate the effects of UHD benzilaminopurine (BA) on Lippia alba shoot culture. Nodal segments obtained from plants growth in vitro was subcultured to Murashigue & Skoog semi-solid medium added with 2ml of these different solutions: BA 3µmol, BA 12CH (10-24), water 12CH and water (no dilution and succussion). Weekly 1 ml of solutions were added to cultures. The experiment was repeated twice and each one consisted in 3 culture vessel with 5 nodal segments per treatment (n=30). All plants were maintained in growth room under controlled temperature (25°C), light and photoperiod (16L/8D). The tested substances were prepared according to the method of stepwise dilution and succussion as describe in Brazilian Homeopathic Pharmacopoeia. The experiment was blinded all the time. After 60d, plantlets were evaluated for number of shoots, shoot length, rooted plants (%), callus development (%) and fresh biomass. Data were submitted to ANOVA following by Duncan’s and t-test. Plants from water 12CH and BA 12CH increased the number of new shoots and promoted the highest shoot length. By adding BA 3µmol the organogenetic response was inhibited since neither shoot nor root were developed. However, it was observed a significant basal callus development. Plant tissue culture could be adapted for UHD studies. More studies are being conducted in way to analyze other experimental conditions and biochemical/phytochemical parameters.

scholarly journals Plantlets Regeneration from Crown Bud Slicing of Pineapple (Ananas comosus)

2018 ◽  
Vol 10 (3) ◽  
pp. 484-490 ◽  
Author(s):  
Zulkarnain Zulkarnain ◽  
Neliyati Neliyati ◽  
Eliyanti Eliyanti
Keyword(s):  
Tissue Culture ◽  
Adventitious Shoots ◽  
Culture Technique ◽  
Leaf Number ◽  
Ananas Comosus ◽  
Culture Techniques ◽  
Lateral Shoots ◽  
Four Levels ◽  
Tissue Culture Techniques

Pineapple propagation by lateral shoots, suckers or crowns is often confronted with limited number of regenerated seedlings and high diversity in flowering and fruit formation. In order to solve this problem, this study offer an alternative method by using tissue culture techniques. This study aimed to determine the effect of growth regulators on plantlet regeneration from bud slicing of pineapple cv. Tangkit. Four levels of 2.4-D (0.0, 0.001, 0.01 and 0.1 ppm) in combination with BA (0.0, 0.1, 1.0 and 10.0 ppm) were tested on solid MS medium. Cultures were incubated in total darkness for a week followed by transfer to 16-hour photoperiod. Results showed that explants treated with 2,4-D and/or BA succeeded in regenerating adventitious shoots. Average leaf number did not differ significantly among treatments (P = 0.60). Highest leaf number (2.99 ± 0.23) was obtained on medium with 0.01 ppm 2,4-D without BA, followed by 0.1 ppm 2,4-D without BA (2.85 ± 0.33). Meanwhile, roots were only formed on medium with 0.1 ppm 2.4-D without BA (4.2 ± 0.37 per shoot). Thus, complete plantlets were regenerated only on medium supplemented with 0.1 ppm 2,4-D without BA. The growth of plantlets was relatively uniform, and plantlet acclimatization succeeded 100% on Jiffy pots. The finding of optimum concentration of 2.4-D and BA in this study is important to develop standard protocol for in vitro propagation of pineapple cv. Tangkit. Thus, the benefit of producing seeds in large quantities and relatively uniform in growth is made possible through tissue culture technique.

scholarly journals Optimasi Konsentrasi Kinetin dan Benzyl Amino Purine Pada Kultur Tunas Vanili (Vanilla planifolia)

2021 ◽  
Vol 21 (1) ◽  
pp. 54-57
Author(s):  
Dyah Nuning Erawati ◽  
Yusriatul Mawaddah ◽  
Siti Humaida ◽  
Irma Wardati
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Culture Techniques ◽  
Planting Material ◽  
Randomized Design ◽  
Wet Weight ◽  
The Mean ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Vanilla has a potential to be developed through tissue culture techniques to anticipate the limitations of the parent plant as a source of planting material. The in vitro propagation ability of vanilla shoots needs to be controlled with the regulation of Kinetin and Benzyl Amino Purines. The interests of this study are 1) analysis of the response of vanilla explants at several Kinetin concentrations; 2) analysis of the response of vanilla explants at several concentrations of BAP and 3) analysis of the interaction of Kinetin and BAP on the response of vanilla explants to form shoot multiplication. The research was conducted at the Tissue Culture Laboratory Politeknik Negeri Jember from June to December 2020 using a factorial Completely Randomized Design (CRD). Factor 1 was the Kinetin concentration of 0.0, 1.0, 2.0 mg.L-1 and the second factor was the concentration of BAP 0.5, 1.5, 2.5 mg.L-1. The results proved that the fastest shoot multiplication occurred on MS medium + Kinetin 2 mg.L-1 with a mean of 8.7 days after inoculation. The mean number of shoots was 7.6 shoots/explant with the highest average wet weight of 0.9 grams/explant at the addition of BAP 1.5 mg. L-1 at measurement 70 days after inoculation.

scholarly journals Pengaruh Kombinasi Sitokinin dan Gibberelin terhadap Pemanjangan Tunas Jambu Mete (Anacardium Occidentale L.) secara In Vitro

Jurnal BIOMA ◽  
2014 ◽  
Vol 10 (1) ◽  
pp. 34
Author(s):  
Febrina Ariyanti ◽  
Christiani Tumilisar ◽  
Rossa Yunita
Keyword(s):  
Tissue Culture ◽  
Economic Value ◽  
Shoot Elongation ◽  
Anacardium Occidentale ◽  
Culture Techniques ◽  
Randomized Design ◽  
Alternative Techniques ◽  
Completely Randomized Design ◽  
Tissue Culture Techniques

Abstract Cashew (Anacardium occidentale L.) is a plant with high economic value. Conventional propagation of this plant still has obstacles, so an alternative techniques using tissue culture could be tried. One of the factors that determine the success of tissue culture techniques is the type and concentration of growth regulators was used. Growth regulator which have effect on shoot elongation is a cytokinin and gibberellin, this research tried to investigate the influence of combination cytokinin and gibberelin on in vitro shoot elongation of cashew. This research was conducted at BB-Biogen, Bogor on June-November 2010. The method in this research was to design experimental method with completely randomized design. The result was cytokinin could increase the length of shoots and quantity of shoots very well until 4 cm and quantity of shoot for 5. With the most effective cytokinin is zeatin of 5 mg/l.   Key words: Anacardium occidentale L., cytokinin, elongation of shoots, gibberelin

scholarly journals Digital Twins for Tissue Culture Techniques—Concepts, Expectations, and State of the Art

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 447
Author(s):  
Johannes Möller ◽  
Ralf Pörtner
Keyword(s):  
Tissue Culture ◽  
Mathematical Models ◽  
Product Life Cycle ◽  
State Of The Art ◽  
Three Dimensional ◽  
Culture Techniques ◽  
Digital Twins ◽  
On Chip ◽  
Tissue Culture Techniques

Techniques to provide in vitro tissue culture have undergone significant changes during the last decades, and current applications involve interactions of cells and organoids, three-dimensional cell co-cultures, and organ/body-on-chip tools. Efficient computer-aided and mathematical model-based methods are required for efficient and knowledge-driven characterization, optimization, and routine manufacturing of tissue culture systems. As an alternative to purely experimental-driven research, the usage of comprehensive mathematical models as a virtual in silico representation of the tissue culture, namely a digital twin, can be advantageous. Digital twins include the mechanistic of the biological system in the form of diverse mathematical models, which describe the interaction between tissue culture techniques and cell growth, metabolism, and the quality of the tissue. In this review, current concepts, expectations, and the state of the art of digital twins for tissue culture concepts will be highlighted. In general, DT’s can be applied along the full process chain and along the product life cycle. Due to the complexity, the focus of this review will be especially on the design, characterization, and operation of the tissue culture techniques.

scholarly journals IN VITRO MULTIPLICATION OF JABON (ANTHOCEPHALUS CADAMBA (ROXB)) ON VARIOUS CONCENTRATION OF BAP AND IAA

2018 ◽  
Vol 4 (2) ◽  
pp. 60
Author(s):  
Asgar Taiyeb ◽  
Baharuddin Baharuddi
Keyword(s):  
Tissue Culture ◽  
Culture Techniques ◽  
In Vitro Multiplication ◽  
Supportive Environment ◽  
Randomized Design ◽  
The Media ◽  
Completely Randomized Design ◽  
The Difference ◽  
Tissue Culture Techniques

One of problems in the Jabon propagation is the availability of seeds both quality and quantity. Tissue culture technology is one of the alternatives that can be used for the supply of  Jabonseeds to produce organs of plants (buds, leaves, roots). The success of plant tissue culture techniques is determined by the condition of explants, a supportive environment and the addition of growth regulators are expected to provide a response to the cultured explants. This study aimed to know the effect of Benzyl Amino Purine (BAP) and Indole Acetic Acid (IAA) to  in vitro multiplication of Jabon. This research conducted at the Laboratory of Forestry Sciences, Faculty of Forestry, University of Tadulako from March to May 2015. Using a completely randomized design with treatments: 0.1 mg / l IAA + 1 mg / l BAP (JB1), 0.1 mg / l IAA + 1.5 mg / l BAP (JB2), 0.1 mg / l IAA + 2 mg / l BAP ( JB3) and 0.1 mg / l IAA + 2.5 mg / l BAP (JB4). Each treatment was repeated three times to obtain 12 experimental units. The results showed that the difference in response Jabon of treatment tested was the highest number of buds and leaves were in the media added 0.1 mg / l IAA + 1.5 mg / l BAP. Furthermore, the formation of callus obtained in media which added 0.1 mg / l IAA + 1 mg / l BAP.

scholarly journals Micropropagation of Morus cathayana through in vitro culture from local Bogor, West Java, Indonesia

2019 ◽  
Vol 11 (1) ◽  
pp. 18-22 ◽  
Author(s):  
YASINTA RATNA ESTI WULANDARI ◽  
MENWANGI ADRIANASHINTA HARJOSUDIRJO
Keyword(s):  
In Vitro Culture ◽  
Shoot Growth ◽  
Nodal Segments ◽  
Statistical Data Analysis ◽  
Juvenile Period ◽  
West Java ◽  
Culture Techniques ◽  
Morus Spp ◽  
Tissue Culture Techniques

Wulandari YRE, Harjosudirjo MA. 2019. Micropropagation of Morus cathayana through in vitro culture from local Bogor, West Java, Indonesia. Nusantara Bioscience 11: 18-22. Mulberry (Morus spp.) is a dicotyledonous plant known for its medicinal benefits as well as silkworm breeding (Bombyx mori L.) to produce silk. Morus cathayana mainly cultivated due to its high content of 1-deoxynojirimycin, widely utilized as an anti-diabetic agent. However, common practice in planting and maintenance of mulberry generate less profit, contributed to its long juvenile period and high heterozygosity level. Hence the development and cultivation through plant tissue culture techniques are necessary. The purpose of this research was to obtain the best combination of thidiazuron as a plant growth regulator. M. cathayana branches with nodal segments were used as explants. Research stages were explants initiation into Murashige and Skoog media, shoot induction in MS media as control and MS+BAP 1 mg/L+NAA 0.25 mg/L+TDZ 0.1, 0.5, 1 mg/L as treatment media, and statistical data analysis. Greater increase in shoot growth was observed in MS+BAP 1 mg/L+NAA 0.25 mg/L+TDZ 0.5 mg/L media, while the formation of shoots and calluses on the explants grown in control and treatment media showed no significant growth.

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