Influence of Omega-3 and Green Tea Extract on Alcohol-Induced Liver Injury in Rats

2018 ◽

pp. 107-116

Author(s):

Ridha Hassan Hussein ◽

Zana Hassan Ibrahim

Keyword(s):

Liver Injury ◽

Green Tea ◽

Density Lipoprotein ◽

Lipid Profiles ◽

Green Tea Extract ◽

Lipoprotein Cholesterol ◽

Total Serum ◽

Omega 3 ◽

Histological Changes ◽

Tea Extract

Drinking alcoholic beverages recently is common in many parts of the world, liver is a principal organ that involved in toxic effects of alcohol and it remains a serious health problem globally. Fatty liver (steatosis) was induced in male albino rats by alcohol 40% orally (3.76gm/kgm BW/day) for the period of 4 weeks, then green tea extract (GTE) and omega-3 (OMG-3) fish oil were used as treatments for improvement and investigating their comparison potential role for the next 4 weeks. At the end of the experiment, the rats were fasted overnight, blood samples through the cardiac puncture and liver organ were collected for biochemical and histopathological analysis respectively. Biochemical parameters including: lipid profiles: (total cholesterol (TC), triglycerides (TG), high density lipoprotein-cholesterol (HDL-C) and low density lipoprotein-cholesterol (LDL-C), serum malondialdehyde (MDA), liver function tests (LFT): (Serum albumin (ALB), serum total protein (TP), total serum bilirubin (TSB), serum alkaline phosphatase (ALP), serum alanine aminotransferase (ALT), and serum aspartate aminotransferase (AST) and finally hepatic histological changes were also investigated. Alcoholic rats were associated with significant elevation (P<0.05) in the levels of serum TC, TG, LDL-C, MDA, TSB, ALT, AST and ALP, whereas the levels of serum HDL-C and TP significantly (P<0.05) decreased with no significant change (P>0.05) of serum ALB level. treatment of alcohol-fed rats with GTE and OMG-3 oil either alone or in their combination have significant role (P<0.05) in lowering the injury effects of alcohol evidenced by reversing the results obtained on serum lipid profiles, MDA, LFTs and hepatic histological changes after their treatments during the amelioration study. Ethanol consumption impaired hepatic functions, disturbed lipid metabolism, induced hyperlipidemia and increased oxidative stress. Aqueous extracts of GT and OMG-3 oil significantly improved alcohol-induced liver injury through improvement of serum markers of hepatic injury, their hypolipidemic actions and hepatic histologic recovery. Co-treatments of GT+OMG-3 have more protective effect or faster progression against ALD than their treatments alone.

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Abstract P423: Effects of Green Tea and Soy Isoflavones on Plasma Lipids In Healthy Subjects

Circulation ◽

10.1161/circ.129.suppl_1.p423 ◽

2014 ◽

Vol 129 (suppl_1) ◽

Author(s):

Miao Hu ◽

Elaine Wat ◽

Clara Lau ◽

Brian Tomlinson

Keyword(s):

Blood Pressure ◽

Green Tea ◽

Plasma Lipids ◽

Density Lipoprotein ◽

Green Tea Extract ◽

Soy Isoflavones ◽

Lipoprotein Cholesterol ◽

Once Daily ◽

Tea Extract ◽

Male Subjects

Introduction: Green tea and soy products are widely consumed in daily life and are considered to have beneficial effects on reducing cardiovascular risks. The flavonoids present in soya bean and tea leaves are believed to be responsible for the proposed benefits. Hypothesis: We assessed the hypothesis that epigallocatechin gallate (EGCG), the most abundant active component of green tea and soy isoflavonoids may reduce the plasma lipid levels in healthy volunteers. Methods: Healthy Chinese male subjects were given green tea extract containing EGCG 800 mg once daily for 14 days and soy isoflavones extract 80 mg once daily for 14 days with a wash-out of at least 4-weeks between the two treatments. Sitting blood pressure and fasting plasma lipids were measured at baseline, after green tea and after soy isoflavones, respectively. Results: In 18 healthy male subjects (mean age 26.6 ± 6.0 years, weight 61.7 ± 6.3 kg), consumption of green tea extract, but not soy isoflavones, was associated with significant reductions in plasma low-density lipoprotein cholesterol (LDL-C) (8.1% [95% confidence interval: -2.0, -14.3%], P<0.01) and total cholesterol (4.8 % [0.4,-10.1%], P<0.05) compared to baseline (Table 1). Reduction in LDL-C with green tea was observed in 15 out of 18 subjects and the change in LDL-C was not affected by the baseline levels. Neither product influenced the plasma high-density lipoprotein cholesterol (HDL-C) or triglyceride levels or blood pressure in this normotensive group of subjects (Table 1). Conclusion: This study showed that short term treatment with a high dose of green tea extract, which is equivalent to about 7-10 cups of green tea, reduced plasma LDL-C which may be beneficial for reducing cardiovascular risk.

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