scholarly journals PEMANFAATAN BAKTERI INDIGENUS SEBAGAI EFFECTIVE MICROORGANISM (EM) UNTUK MEMPERCEPAT LAJU DEKOMPOSISI SAMPAH ORGANIK

2019 ◽  
Vol 2 (3) ◽  
pp. 53
Author(s):  
Hastari Nastiti

Recycling of organic waste is important because it can reduce environmental problems. Composting is the alternative method for waste recycling, but naturally, the processes always waste a time. EM technology is proven to decrease the composting time. This research conducts the isolation for 3 kinds of indigenous bacteria from compost that used for liquid EM production. The yield of isolation process are 3.6x109 cfu/g phosphate degrading bacteria, 5.6x109 cfu/g cellulose degrading bacteria and       2.7x109 cfu/g nitrogen fixing bacteria. The medium are used for this study to make EM contain of aquadest, 25% sugar cane fresh liquid and 2% Nitrogen-Phosphor-Potassium mixing fertilizer. The final EM product contain of 8.2x108 cfu/g mixing bacteria. The solid starter production with liquid EM addition needs 14 days. The final solid starter has brown color and smell like soil, roughly texture but break up, 7.8 for pH average and 33.8oC for temperature average. In other hand, fresh compost production need 22 days. Further more, in final process, the fresh compost has black-brown color and smell like soil, smoothly texture and crumbs, humidity decreasing, 7.4 for pH average and  44.5oC for temperature average.

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Rahmawati Ridwan ◽  
Dedik Budianta ◽  
Hary Widjajanti

The study was conducted to determine the effect of land typology and lime on the population of indigenous nitrogen-fixing bacteria and soil pH, and to analyze the effect of soil typology interaction and lime on the population of indigenous nitrogen-fixing bacteria and soil pH. Sampling of soil in tidal land of Mulya Sari and Banyu Urip Village of Banyuasin Regency, South Sumatera. Then the sample was studied at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Inderalaya. During January-May 2017. Based on the results of the study known the soil typology of the total population of nitrogen-fixing bacteria, the highest population of nitrogen inhibitor bacteria in the second week of soil typology D was 6.0x107 and the highest pH of 5.18 in typology B on week Third, the lime treatment that affected the population of indigenous nitrogen-fixing bacteria, obtained the population of bacteria as much as 9.3x105 in the second week and the highest soil pH 4.93 in the second week, while the results of the interaction between soil typology and lime treatment only affect the population of bacterial inhibitor Indigenous nitrogen, with bacterial population of 3.5x107 in soil typology D in the second week, but this interaction did not significantly affect soil pH, with the highest pH measurement of 5.33 on the soil typology D.Keywords: nitrogen-fixing bacteria, tidal land, lime treatment


2006 ◽  
Vol 12 (1) ◽  
pp. 31-39
Author(s):  
Evelina Ivanova ◽  
Ernest Teunou ◽  
Denis Poncelet

This paper presents the first steps of the development of dry capsules of biofertilizer to replace chemical fertilizers that cause environmental problems. This new product consists of macrocapsules (large size beads about 3 to 4 mm diameter) containing nitrogen fixing bacteria: Azospirillum. They are produced by quick encapsulation and drying processes, are made of alginate (3%), standard starch (44.6%) and modified starch (2.4%) and can contain up to 106 CFU/capsule. This large size inoculum carrier was formulated to reduce production costs, favour its storage and application in the field and the results show that this new form of inoculum carrier is far better than the liquid and powdered forms.


2014 ◽  
Vol 71 (3) ◽  
pp. 171-180 ◽  
Author(s):  
Bruno Lima Soares ◽  
Paulo Avelar Ademar Ferreira ◽  
Silvia Maria de Oliveira-Longatti ◽  
Leandro Marciano Marra ◽  
Marcia Rufini ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luz K. Medina-Cordoba ◽  
Aroon T. Chande ◽  
Lavanya Rishishwar ◽  
Leonard W. Mayer ◽  
Lina C. Valderrama-Aguirre ◽  
...  

AbstractPrevious studies have shown the sugarcane microbiome harbors diverse plant growth promoting microorganisms, including nitrogen-fixing bacteria (diazotrophs), which can serve as biofertilizers. The genomes of 22 diazotrophs from Colombian sugarcane fields were sequenced to investigate potential biofertilizers. A genome-enabled computational phenotyping approach was developed to prioritize sugarcane associated diazotrophs according to their potential as biofertilizers. This method selects isolates that have potential for nitrogen fixation and other plant growth promoting (PGP) phenotypes while showing low risk for virulence and antibiotic resistance. Intact nitrogenase (nif) genes and operons were found in 18 of the isolates. Isolates also encode phosphate solubilization and siderophore production operons, and other PGP genes. The majority of sugarcane isolates showed uniformly low predicted virulence and antibiotic resistance compared to clinical isolates. Six strains with the highest overall genotype scores were experimentally evaluated for nitrogen fixation, phosphate solubilization, and the production of siderophores, gibberellic acid, and indole acetic acid. Results from the biochemical assays were consistent and validated computational phenotype predictions. A genotypic and phenotypic threshold was observed that separated strains by their potential for PGP versus predicted pathogenicity. Our results indicate that computational phenotyping is a promising tool for the assessment of bacteria detected in agricultural ecosystems.


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