scholarly journals IDENTIFIKASI BAKTERI PEREDUKSI SULFAT PADA KAWAH AIR PANAS NIRWANA SUOH LAMPUNG BARAT

BIOLOVA ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 122-127
Author(s):  
Beny Saputra ◽  
Agus Sutanto ◽  
Mia Cholvistaria ◽  
Suprayitno Suprayitno ◽  
Nala Rahmawati
Keyword(s):  
Organic Matter ◽  
Electron Donor ◽  
Hot Spring ◽  
Anaerobic Bacteria ◽  
Reduction Process ◽  
Sulfate Reducing Bacteria ◽  
Organic Substrates ◽  
Sulfate Reducing ◽  
Obligate Anaerobic ◽  
Reducing Bacteria

Abstrak: Bakteri pereduksi sulfat atau Sulfate-reducing bacteria (SRB) adalah jenis bakteri obligat anaerob kemolitrotof memanfaatkan donor electron H2. Kemampuan SRB mereduksi sulfat menjadi sulfida mampu mengendapkan logam toksik meliputi Cd, Cu, dan Zn sebagai logam sulfida. SRB memerlukan substrat organik seperti asam piruvat yang dihasilkan oleh aktivitas anaerob lainnya. Mekanisme SRB dalam melakukan reduksi sulfat, sulfat digunakan sebagai sumber energi sebagai akseptor elektron dan menggunakan sumber karbon (C) sebagai donor elekton dalam metabolisme dan bahan penyusun sel. Pada kondisi anaerob bahan organik akan berperan sebagai donor elektron. Pembentukan senyawa sulfida melalui proses reduksi yang ditandai oleh penambahan elektron dari bahan organik yang menyebabkan turunnya konsentrasi sulfat dan naiknya pH lingkungan. SRB pada kawah air panas nirwana ini hidup secara anaerob pada suhu lingkungan 600C - 1000C dengan pH 7,4 tingkat kekeruhan air cukup keruh dan kandungan air yang mengandung blerang dengan indikator bau seperti telur busuk dan lingkungan sekitar terdiri dari sedimen batu kapur.   Abstract : Sulfate-reducing bacteria (BPS) is a type of chemolithotroph obligate anaerobic bacteria that utilize H2 electron donors. The ability of BPS to reduce sulfate to sulfide is able to precipitate toxic metals including Cd, Cu, and Zn as metal sulfides. BPS requires organic substrates such as pyruvic acid which is produced by other anaerobic activities. The BPS mechanism in reducing sulfate, sulfate is used as an energy source as an electron acceptor and uses a carbon source (C) as an electron donor in metabolism and cell building material. Under anaerobic conditions, organic matter will act as an electron donor. The formation of sulfide compounds through a reduction process is characterized by the addition of electrons from organic matter which causes a decrease in sulfate concentration and an increase in environmental pH. BPS in this nirvana hot spring crater lives anaerobically at an environmental temperature of 600C - 1000C with a pH of 7.4 the level of turbidity of the water is quite cloudy and the water content contains sulfur with an indicator of smell like rotten eggs and the surrounding environment consists of limestone sediments

scholarly journals Competitive Growth of Sulfate-Reducing Bacteria with Bioleaching Acidophiles for Bioremediation of Heap Bioleaching Residue

2020 ◽  
Vol 17 (8) ◽  
pp. 2715 ◽  
Author(s):  
Aung Kyaw Phyo ◽  
Yan Jia ◽  
Qiaoyi Tan ◽  
Heyun Sun ◽  
Yunfeng Liu ◽  
...  
Keyword(s):  
Organic Matter ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Sulfate Reducing Bacteria ◽  
Sulfide Minerals ◽  
Sulfate Reducing ◽  
Heap Bioleaching ◽  
Waste Rocks ◽  
Sulfur Oxidizing ◽  
Reducing Bacteria

Mining waste rocks containing sulfide minerals naturally provide the habitat for iron- and sulfur-oxidizing microbes, and they accelerate the generation of acid mine drainage (AMD) by promoting the oxidation of sulfide minerals. Sulfate-reducing bacteria (SRB) are sometimes employed to treat the AMD solution by microbial-induced metal sulfide precipitation. It was attempted for the first time to grow SRB directly in the pyritic heap bioleaching residue to compete with the local iron- and sulfur-oxidizing microbes. The acidic SRB and iron-reducing microbes were cultured at pH 2.0 and 3.0. After it was applied to the acidic heap bioleaching residue, it showed that the elevated pH and the organic matter was important for them to compete with the local bioleaching acidophiles. The incubation with the addition of organic matter promoted the growth of SRB and iron-reducing microbes to inhibit the iron- and sulfur-oxidizing microbes, especially organic matter together with some lime. Under the growth of the SRB and iron-reducing microbes, pH increased from acidic to nearly neutral, the Eh also decreased, and the metal, precipitated together with the microbial-generated sulfide, resulted in very low Cu in the residue pore solution. These results prove the inhibition of acid mine drainage directly in situ of the pyritic waste rocks by the promotion of the growth of SRB and iron-reducing microbes to compete with local iron and sulfur-oxidizing microbes, which can be used for the source control of AMD from the sulfidic waste rocks and the final remediation.

Organic Substrates in Bioremediation of Acidic Saline Drainage Waters by Sulfate-Reducing Bacteria

2009 ◽  
Vol 209 (1-4) ◽  
pp. 251-268 ◽  
Author(s):  
Talitha C. Santini ◽  
Brad P. Degens ◽  
Andrew W. Rate
Keyword(s):  
Sulfate Reducing Bacteria ◽  
Organic Substrates ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Drainage Waters

Arsenic removal and activity of a sulfate reducing bacteria-enriched anaerobic sludge using zero valent iron as electron donor

2020 ◽  
Vol 384 ◽  
pp. 121392 ◽  
Author(s):  
Olga Lidia Zacarías-Estrada ◽  
Lourdes Ballinas-Casarrubias ◽  
María Elena Montero-Cabrera ◽  
Rene Loredo-Portales ◽  
Erasmo Orrantia-Borunda ◽  
...  
Keyword(s):  
Electron Donor ◽  
Arsenic Removal ◽  
Sulfate Reducing Bacteria ◽  
Zero Valent Iron ◽  
Anaerobic Sludge ◽  
Sulfate Reducing ◽  
Reducing Bacteria

scholarly journals Stable Carbon Isotope Fractionation by Sulfate-Reducing Bacteria

2003 ◽  
Vol 69 (5) ◽  
pp. 2942-2949 ◽  
Author(s):  
Kathleen L. Londry ◽  
David J. Des Marais
Keyword(s):  
Carbon Isotope ◽  
Isotope Fractionation ◽  
Carbon Isotope Discrimination ◽  
Stable Carbon Isotope ◽  
Reducing Power ◽  
Sulfate Reducing Bacteria ◽  
Organic Substrates ◽  
Isotope Discrimination ◽  
Sulfate Reducing ◽  
Reducing Bacteria

ABSTRACT Biogeochemical transformations occurring in the anoxic zones of stratified sedimentary microbial communities can profoundly influence the isotopic and organic signatures preserved in the fossil record. Accordingly, we have determined carbon isotope discrimination that is associated with both heterotrophic and lithotrophic growth of pure cultures of sulfate-reducing bacteria (SRB). For heterotrophic-growth experiments, substrate consumption was monitored to completion. Sealed vessels containing SRB cultures were harvested at different time intervals, and δ13C values were determined for gaseous CO2, organic substrates, and products such as biomass. For three of the four SRB, carbon isotope effects between the substrates, acetate or lactate and CO2, and the cell biomass were small, ranging from 0 to 2‰. However, for Desulfotomaculum acetoxidans, the carbon incorporated into biomass was isotopically heavier than the available substrates by 8 to 9‰. SRB grown lithoautotrophically consumed less than 3% of the available CO2 and exhibited substantial discrimination (calculated as isotope fractionation factors [α]), as follows: for Desulfobacterium autotrophicum, α values ranged from 1.0100 to 1.0123; for Desulfobacter hydrogenophilus, the α value was 0.0138, and for Desulfotomaculum acetoxidans, the α value was 1.0310. Mixotrophic growth of Desulfovibrio desulfuricans on acetate and CO2 resulted in biomass with a δ13C composition intermediate to that of the substrates. The extent of fractionation depended on which enzymatic pathways were used, the direction in which the pathways operated, and the growth rate, but fractionation was not dependent on the growth phase. To the extent that environmental conditions affect the availability of organic substrates (e.g., acetate) and reducing power (e.g., H2), ecological forces can also influence carbon isotope discrimination by SRB.

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