Simultaneous Estimation of Ambroxol Hydrochloride and Cetirizine Hydrochloride in Combined Solid Tablet Formulations by HPTLC- Densitometric Method

2019 ◽  
Vol 9 (1) ◽  
Keyword(s):  
Simultaneous Estimation ◽  
Cetirizine Hydrochloride ◽  
Tablet Formulations ◽  
Ambroxol Hydrochloride

scholarly journals Simultaneous Estimation of Ambroxol Hydrochloride and Cetirizine Hydrochloride in Pharmaceutical Tablet Dosage Form by Simultaneous Equation Spectrophotometric Method: A Quality Control Tool for Dissolution Studies

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Deepak Sharma ◽  
Mankaran Singh ◽  
Dinesh Kumar ◽  
Gurmeet Singh
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Drug Formulation ◽  
Simultaneous Estimation ◽  
Validation Parameters ◽  
Quality Control Tool ◽  
Cetirizine Hydrochloride ◽  
Ambroxol Hydrochloride ◽  
Tablet Dosage

Ambroxol Hydrochloride and Cetirizine Hydrochloride are used for the treatment of bronchitis, cough, and allergy. A simple, economical, accurate, and precise method for simultaneous estimation of Ambroxol Hydrochloride and Cetirizine Hydrochloride in tablet dosage form has been developed. Simultaneous equation method based on measurement of absorbance at two wavelengths, that is, 244 nm and 230 nm, λmax of Ambroxol Hydrochloride and Cetirizine Hydrochloride in pH 6.8 phosphate buffer. Both of these drugs obeyed Beer-Lambert’s law in the concentration range of 2–18 µg/mL for Ambroxol Hydrochloride and 2–20 µg/mL for Cetirizine Hydrochloride. The high values of correlation coefficient (R) indicated good linearity of calibration curve for both the drugs. The accuracy and precision of method were determined and the method was validated statistically. Result of percentage recovery study confirms the accuracy of proposed method. As per the ICH guidelines, the method validation parameters checked were linearity, accuracy, precision, and assay of drug formulation. Based on the results obtained, it can be concluded that the proposed simultaneous equation spectrophotometric method for simultaneous estimation of Ambroxol Hydrochloride and Cetirizine Hydrochloride is rapid, economical, accurate, precise, and reproducible. Hence, the proposed method can be employed for quantitative estimation of Ambroxol Hydrochloride and Cetirizine Hydrochloride from their tablet dosage form.

CHEMOMETRICS ASSISTED STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF ACETYL CYSTEINE AND AMBROXOL HYDROCHLORIDE IN BULK AND DOSAGE FORM

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (03) ◽  
pp. 46-53
Author(s):  
H. Potluri ◽  
Keyword(s):  
Dosage Form ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Tablet Formulations ◽  
Acetyl Cysteine ◽  
Ambroxol Hydrochloride ◽  
Full Factorial

A simple and precise stability indicating RP-HPLC method was developed for the simultaneous estimation of acetyl cysteine and ambroxol hydrochloride in bulk and dosage form. Experimental design based evaluation using a 23 full factorial design was applied to evaluate coefficient, ANOVA for the establishment of robustness nature of the method. Kromosil (250 mm x 4.6 mm, 5 μ) C18 column at 274 nm of UV detection was used. A composition of 0.1 % ortho phosphoric acid and acetonitrile in the ratio of 28:72 (V:V) was used as the mobile phase with a flow rate of 1.0 mL min-1. Linearity was established over the concentration range of 50-300 μg. mL-1 for acetyl cysteine and 7.5-45 μg mL-1 for ambroxol hydrochloride. The proposed method was validated and was successfully utilized for the quantitative analysis of tablet formulations containing acetyl cysteine and ambroxol hydrochloride.

scholarly journals Simultaneous Assay of Olanzapine and Fluoxetine in Tablets by Column High-Performance Liquid Chromatography and High-Performance Thin-Layer Chromatography

2007 ◽  
Vol 90 (6) ◽  
pp. 1573-1578 ◽  
Author(s):  
Charmy R Shah ◽  
Nehal J Shah ◽  
Bhanubhai N Suhagia ◽  
Natvarlal M Patel
Keyword(s):  
Thin Layer ◽  
Mobile Phase ◽  
High Performance ◽  
Potassium Dihydrogen Phosphate ◽  
High Performance Liquid Chromatographic ◽  
Ultraviolet Absorption ◽  
Simultaneous Estimation ◽  
Dihydrogen Phosphate ◽  
Aluminum Sheets ◽  
Tablet Formulations

Abstract This paper describes validated high-performance liquid chromatographic (LC) and high-performance thin-layer chromatographic (TLC) methods for the simultaneous estimation of olanzapine and fluoxetine in pure powder and tablet formulations. The LC separation was achieved on a Lichrospher 100 RP-180, C18 column (250 mm, 4.0 mm id, 5 m) using 0.05 M potassium dihydrogen phosphate buffer (pH 5.6 adjusted with o-phosphoric acid) acetonitrile (50 + 50, v/v) as the mobile phase at a flow rate of 1 mL/min and ambient temperature. The TLC separation was achieved on aluminum sheets coated with silica gel 60F254 using methanoltoluene (40 + 20, v/v) as the mobile phase. Quantitation was achieved by measuring ultraviolet absorption at 233 nm over the concentration range of 1070 and 40280 g/mL with mean recovery of 99.54 0.89 and 99.73 0.58% for olanzapine and fluoxetine, respectively, by the LC method. Quantitation was achieved by measuring ultraviolet absorption at 233 nm over the concentration range of 100800 and 4003200 ng/spot with mean recovery of 101.53 0.06 and 101.45 0.35% for olanzapine and fluoxetine, respectively, by the TLC method with densitometry. These methods are simple, precise, and sensitive, and they are applicable for simultaneous determination of olanzapine and fluoxetine in tablet formulations.

scholarly journals RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF FINASTERIDE AND TAMSULOSIN IN TABLET FORMULATIONS

2015 ◽  
Vol 26 (1) ◽  
pp. 45
Author(s):  
Ashu Mittal ◽  
Shikha Parmar ◽  
Syed Sarim Imam ◽  
Sadaf J. Gilani ◽  
Mohd Aqil
Keyword(s):  
Hplc Method ◽  
Simultaneous Estimation ◽  
Rp Hplc ◽  
Tablet Formulations

Spectrophotometric estimation of ambroxol hydrochloride and cetirizine hydrochloride in tablets

2008 ◽  
Vol 2 (3) ◽  
pp. 159 ◽  
Author(s):  
NeelaManish Bhatia ◽  
SantoshKumar Ganbavale ◽  
HarinathNivrutti More
Keyword(s):  
Cetirizine Hydrochloride ◽  
Ambroxol Hydrochloride

Stability Indicating Simultaneous Estimation of Cetirizine Hydrochloride and Phenylephrine Hydrochloride in Combined tablet formulation by using HPTLC- Densitometric method

2021 ◽  
pp. 2467-2471
Author(s):  
M.R. Bodhankar ◽  
S.J. Wadher ◽  
T.M. Kalyankar ◽  
Anitha K
Keyword(s):  
High Performance ◽  
Heat Exposure ◽  
Calibration Plot ◽  
Simultaneous Estimation ◽  
Chromatography Method ◽  
Phenylephrine Hydrochloride ◽  
Solid Dosage ◽  
Cetirizine Hydrochloride ◽  
Layer Chromatography ◽  
Hptlc Method

The new simple, precise, rapid, selective, high performance thin layer chromatography method has been developed for simultaneous estimation of cetirizine hydrochloride and phenylephrine hydrochloride in its combined solid dosage form. The HPTLC method was performed on precoated silica gel G60 F254 plates with, Toluene: Methanol: Ammonia (8: 2: 1, v/v/v) as optimum mobile phase. The plates were developed in 6.00× 0.30mm at room temperature. The developed plates were scanned and quantified at their absorption at 224 nm for CET and PHE respectively. The drugs were satisfactorily resolved with Rf = 0.36± 0.001 for CET and 0.65±0.004 for PHE. Calibration plot was linear between 100-600 ng/ spot for CET and 200-1200 ng/ spot for PHE. This HPTLC method is sensitive, economical, less time consuming than other chromatographic procedures. It can become important tool for analysis of combined solid dosage from. In addition to this results acid exposure, base exposure, oxidative exposure, neutral exposure, photolysis and dry heat exposure is carried out.

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