Validated Derivative Spectrophotometric method for simultaneous estimation of Levocetirizine Dihydrochloride and Phenylephrine Hydrochloride from tablet formulations

2019 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
G. K. Dyade
Keyword(s):  
Spectrophotometric Method ◽  
Simultaneous Estimation ◽  
Phenylephrine Hydrochloride ◽  
Tablet Formulations

scholarly journals Development and Validation of First-Order Derivative Spectrophotometry for Simultaneous Determination of Levocetirizine Dihydrochloride and Phenylephrine Hydrochloride in Pharmaceutical Dosage Form

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Kaminee Parmar ◽  
Sunil Baldania ◽  
Dimal Shah ◽  
Usmangani Chhalotiya ◽  
Naimin Parmar
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Correlation Coefficients ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Zero Crossing ◽  
Phenylephrine Hydrochloride ◽  
First Order ◽  
Crossing Point

A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of levocetirizine dihydrochloride (LCT) and phenylephrine hydrochloride (PHE) by employing first-order derivative spectrophotometric method. The first-order derivative absorption at 240 nm (zero crossing point of PHE) was used for quantification of LCT and 283.2 nm (zero crossing point of LCT) for quantification of PHE. The linearity was established over the concentration range of 4–24 μg/mL and 8–48 μg/mL for LCT and PHE with correlation coefficients (r2) 0.9964 and 0.9972, respectively. The mean % recoveries were found to be in the range of 99.14%–100.43% for LCT and 98.73%–100.83% for PHE. The proposed method has been validated as per ICH guideline and successfully applied for the simultaneous estimation of LCT and PHE in combined tablet dosage form.

scholarly journals Simultaneous Estimation of Naproxen and Ranitidine HCl by Using UV Spectrophotometer

1970 ◽  
Vol 1 (1) ◽  
pp. 18-24 ◽  
Author(s):  
Tasnuva Haque ◽  
Md Mesbah Uddin Talukder ◽  
Susmita Laila ◽  
Kanij Fatema ◽  
Abdul Kalam Lutful Kabir
Keyword(s):  
Key Words ◽  
Spectrophotometric Method ◽  
Phosphate Buffer ◽  
Simultaneous Estimation ◽  
Ultraviolet Spectroscopy ◽  
Absorbance Measurement ◽  
Tablet Formulations

The development of a UV Spectrophotometric method for simultaneous estimation of Ranitidine HCl and Naproxen involves absorbance measurement of Ranitidine HCl at 313 nm in pH 7.4 phosphate buffer and 314 nm in both 0.1N HCl and in water and that of Naproxen at 229 nm in pH 7.4 phosphate buffer and 232 nm in both 0.1N HCl and in water corresponding to the respective absorption maxima. Both the drugs obey Beer- Lambert's law in the range of 5-25 μg/ml for Ranitidine HCl and 0.2-1.25 μg/ml for Naproxen. The method developed was validated to determine its linearity, precision, reproducibility and sensitivity. The tablet formulations were evaluated for the percent content of both the drugs at the selected wavelengths and the percent potency were 98.83 and 99.15 for Naproxen and Ranitidine HCl respectively. Key words: Naproxen, Ranitidine HCl, Ultraviolet spectroscopy, marketed products.doi:10.3329/sjps.v1i1.1781   S. J. Pharm. Sci. 1(1&2): 18-24

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF NAPHAZOLINE HCL AND PHENYLEPHRINE HCL IN EYE DROPS

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (06) ◽  
pp. 26-31 ◽  
Author(s):  
Satish A Patel ◽  
Dharmendrasinh A Baria
Keyword(s):  
Spectrophotometric Method ◽  
Cost Effective ◽  
Simultaneous Estimation ◽  
Eye Drops ◽  
Crossover Point ◽  
Phenylephrine Hydrochloride ◽  
First Order ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Development And Validation

A first order derivative UV-spectrophotometric method was developed to quantify naphazoline hydrochloride and phenylephrine hydrochloride in eye drops. Naphazoline hydrochloride and phenylephrine hydrochloride were estimated at 275.6nm (zero crossover point of phenylephrine hydrochloride) and 289.4nm (zero crossover point of naphazoline hydrochloride), respectively. The method was found to be linear in the concentration range of 10 - 25 µg/ml for naphazoline hydrochloride and 20 - 60 µg/ml for phenylephrine hydrochloride. The developed method was subjected to validation in accordance with ICH guidelines Q2(R1) for different validation parameters. The analytical method developed was found to be accurate and cost - effective and can be used for routine analysis of naphazoline hydrochloride and phenylephrine hydrochloride in eye drops.

DEVELOPMENT OF ULTRAVIOLET SPECTROPHOTOMETRIC METHOD FOR THE SIMULTANEOUS ESTIMATION OF BRIMONIDINE TARTRATE AND BRINZOLAMIDE

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (11) ◽  
pp. 29-34
Author(s):  
V. P Agrawal ◽  
◽  
S. S Desai ◽  
G. K. Jani
Keyword(s):  
Spectrophotometric Method ◽  
Simultaneous Estimation ◽  
Brimonidine Tartrate

A simple, accurate and precise UV spectrophotometric method has been developed for the simultaneous estimation of brimonidine tartrate (BRT) and brinzolamide (BRZ) using methanol as solvent. The λmax for BRT and BRZ were found to be 244 nm and 253 nm, respectively. The linearity was found in the range of 1-7 μg/mL and 5- 35 μg/mL for BRT and BRZ, respectively. The LOD and LOQ for BRT were found to be 0.0149 µg/mL and 0.0425 µg/mL at 244 nm and 0.0278 µg/mL and 0.0843 µg/mL at 253 nm, respectively whereas those for BRZ were found to be 0.0330 µg/mL and 0.1000 µg/mL at 244 nm and 0.0243 µg/mL and 0.0737 µg/mL at 253 nm, respectively. The % recovery of the drugs by the proposed method was found in the range of 98.29% to 100.82%. The method was found to be precise and robust. The method was successfully applied for determination of BRT and BRZ in an in-house suspension.

DETERMINATION OF TELMISARTAN HYDROCHLORIDE AND HYDROCHLOROTHIAZIDE IN BINARY MIXTURE AND PHARMACEUTICAL TABLET DOSAGE FORM BY FIRST DERIVATIVE RATIO SPECTROPHOTOMETRIC METHOD

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (11) ◽  
pp. 57-64
Author(s):  
V Thakur ◽  
◽  
S. J. Daharwal
Keyword(s):  
Binary Mixture ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
First Derivative ◽  
Pharmaceutical Tablet ◽  
Study Results ◽  
Recovery Study ◽  
Tablet Dosage

New first derivative ratio spectrophotometric method was developed for simultaneous estimation of telmisartan hydrochloride (TELM) and hydrochlorothiazide (HCTZ) in combined tablet dosage form without prior separation. The amplitude in the first derivative of the corresponding ratio spectra at 263.2 nm and 278.4 nm of TELM and for HCTZ at 287.2 nm and 304.8 nm were selected for the determination. The calibration graphs were established in the range of 1-10μg/mL and 2-15 μg/mL of TELM and HCTZ, respectively. The percentage recoveries of binary mixture of TELM were found to be 97.81±1.05 (263.2nm) and 96.60±1.91 (278.4nm) and for HCTZ were found to be 97.47±1.41 (287.2), 98.16± 1.45 (304.8nm) by proposed method. This method was successfully applied for the analysis of commercial tablet formulation, with no interference from excipients as indicated by the recovery study results. The proposed method was validated, statistically analyzed, compared with reported HPLC method and can be successfully applied for the routine analysis.

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