scholarly journals HIV reverse transcriptases defective in strand displacement activity

IBJ Plus ◽  
2021 ◽  
Author(s):  
◽  
◽  
Samara Martín-Alonso ◽  
Mar Álvarez ◽  
Luis Menéndez-Arias ◽  
...  
Keyword(s):  
Strand Displacement ◽  
Displacement Activity ◽  
Reverse Transcriptases

scholarly journals Detection of SARS-CoV-2 using non-commercial RT-LAMP regents and raw samples.

2020 ◽  
Author(s):  
Alisa Alekseenko ◽  
Donal Barrett ◽  
Yerma Pareja-Sanchez ◽  
Rebecca Howard ◽  
Emilia Strandback ◽  
...  
Keyword(s):  
Dna Polymerases ◽  
Scale Up ◽  
Lamp Assay ◽  
Cost Effective ◽  
Economic Resources ◽  
Strand Displacement ◽  
Reaction Conditions ◽  
Clinical Patient ◽  
Displacement Activity ◽  
Reverse Transcriptases

RT-LAMP detection of SARS-CoV-2 has been shown as a valuable approach to scale up COVID-19 diagnostics and thus contribute to limiting the spread of the disease. Here we present the optimization of highly cost-effective in-house produced enzymes, and we benchmark their performance against commercial alternatives. We explore the compatibility between multiple DNA polymerases with high strand-displacement activity and thermostable reverse transcriptases required for RT-LAMP. We optimize reaction conditions and demonstrate their applicability using both synthetic RNA and clinical patient samples. Finally, we validated the optimized RT-LAMP assay for the detection of SARS-CoV-2 in raw nasopharyngeal samples from 184 patients. We anticipate that optimized and affordable reagents for RT-LAMP will facilitate the expansion of SARS-CoV-2 testing globally, especially in sites and settings with limited economic resources.

scholarly journals Strand Displacement Activity of PrimPol

2020 ◽  
Vol 21 (23) ◽  
pp. 9027
Author(s):  
Elizaveta O. Boldinova ◽  
Ekaterina A. Belousova ◽  
Diana I. Gagarinskaya ◽  
Ekaterina A. Maltseva ◽  
Svetlana N. Khodyreva ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Strand Displacement ◽  
Displacement Activity

Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn2+ ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5′-DNA flaps generated by PrimPol in vitro.

scholarly journals 3′ to 5′ Exonuclease Activity of Herpes Simplex Virus Type 1 DNA Polymerase Modulates Its Strand Displacement Activity

2003 ◽  
Vol 77 (18) ◽  
pp. 10147-10153 ◽  
Author(s):  
Yali Zhu ◽  
Kelly S. Trego ◽  
Liping Song ◽  
Deborah S. Parris
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Dna Polymerase ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Strand Displacement ◽  
Displacement Activity ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT Using a minicircle DNA primer-template, the wild-type catalytic subunit of herpes simplex virus type 1 (HSV-1) DNA polymerase (pol) was shown to lack significant strand displacement activity with or without its processivity factor, UL42. However, an exonuclease-deficient (exo−) pol (D368A) was capable of slow strand displacement. Although UL42 increased the rate (2/s) and processivity of strand displacement by exo− pol, the rate was slower than that for gap-filling synthesis. High inherent excision rates on matched primer-templates and rapid idling-turnover (successive rounds of excision and polymerization) of exo-proficient polymerases correlated with poor strand displacement activity. The results suggest that the exo activity of HSV-1 pol modulates its ability to engage in strand displacement, a function that may be important to the viability and genome stability of the virus.

scholarly journals A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques ◽  
2014 ◽  
Vol 57 (2) ◽  
Author(s):  
Konstantin B. Ignatov ◽  
Ekaterina V. Barsova ◽  
Arkady F. Fradkov ◽  
Konstantin A. Blagodatskikh ◽  
Tatiana V. Kramarova ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Amplification ◽  
Strand Displacement ◽  
Displacement Activity ◽  
Thermostable Dna Polymerase

scholarly journals Pif1 removes a Rap1-dependent barrier to the strand displacement activity of DNA polymerase δ

2016 ◽  
Vol 44 (8) ◽  
pp. 3811-3819 ◽  
Author(s):  
Katrina N. Koc ◽  
Saurabh P. Singh ◽  
Joseph L. Stodola ◽  
Peter M. Burgers ◽  
Roberto Galletto
Keyword(s):  
Dna Polymerase ◽  
Strand Displacement ◽  
Displacement Activity ◽  
Dna Polymerase Δ

scholarly journals DNA-directed DNA Polymerase and Strand Displacement Activity of the Reverse Transcriptase Encoded by the R2 Retrotransposon

2007 ◽  
Vol 374 (2) ◽  
pp. 322-333 ◽  
Author(s):  
Anna Kurzynska-Kokorniak ◽  
Varuni K. Jamburuthugoda ◽  
Arkadiusz Bibillo ◽  
Thomas H. Eickbush
Keyword(s):  
Reverse Transcriptase ◽  
Dna Polymerase ◽  
Strand Displacement ◽  
Displacement Activity
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