scholarly journals LEUKEMIA SEL BERAMBUT

2018 ◽  
Vol 19 (2) ◽  
pp. 132
Author(s):  
Reini Meilani Isbach ◽  
Agus Alim Abdullah ◽  
Mansyur Arif
Keyword(s):  
Peripheral Blood ◽  
Disease Onset ◽  
Peripheral Blood Smear ◽  
Cell Leukaemia ◽  
Definite Diagnosis ◽  
Hairy Cell ◽  
Blood Smears ◽  
Laboratory Results ◽  
Hairy Cells ◽  
Peripheral Blood Smears

Hairy cell leukaemia (HCL) is a neoplastic disorder of B lymphocytes originally described by Bouroncle et al. in 1958. HCL clinicalmanifestations varies, generally characterized by various degrees of splenomegaly, pancytopenia, or emphasis only on the two cell lines(bisitopenia), with the hairy cells in varying amounts in the peripheral blood smear and bone marrow. HCL is a very rare case, there areonly about 2% of all leukaemias more frequently in men than women (4:1) with the average age of disease onset between 50–55 years.The etiology of HCL is still not known. A case of HCL Leukaemia in a female patient, aged 55 years is reported which was a rare case.HCL diagnosis in this patient was based on the clinical manifestation (splenomegaly), and laboratory results (bisitopenia, neutropeniaand monositopenia) and about 80% hairy cells were found in peripheral blood smears. Definite diagnosis of HCL should be made by bonemarrow examination, immunophenotyping and cytogenesis.

scholarly journals Evaluation of EDTA induced pseudothrombocytopenia and the effect of alternative anticoagulants

2014 ◽  
Vol 4 (8) ◽  
pp. 626-629
Author(s):  
A Shrestha ◽  
S Karki
Keyword(s):  
Platelet Count ◽  
Peripheral Blood ◽  
Peripheral Blood Smear ◽  
Institute Of Medicine ◽  
Platelet Counts ◽  
Blood Smears ◽  
Low Platelet Count ◽  
The Mean ◽  
Correct Estimation ◽  
Peripheral Blood Smears

Background: Artifactual Thrombocytopenia is a condition in which there is falsely lowered platelet in patients who have thrombocytopenia but the absence of petechiae or echymoses. Pseudothrombocytopenia is also an artifactual thrombocytopenia caused by anticoagulant dependent agglutinins. The aim of this study was to compare the platelet count in pseudothrombocytopenia in EDTA anticoagulated samples and other alternative anticoagulants.Materials and methods: This study was performed in the department of hemotology hematology, Institute of medicine. All cases during study period were evaluated by EDTA-anticoagulated whole blood samples but criteria for selecting pseudothrombocytopenia patients was unexpectedly low platelet counts with clumping/aggregate on peripheral blood smear. Additional samples were collected in sodium citrate and heparin for examined.Results: A total of 50 patients aged between 18 to 90 years were found to have pseudothrombocytopenia. Platelet counts in samples anticoagulated with EDTA ranged from 20x109/l to 149x109/l and samples from same patients anticoagulated with citrate ranged from 41x109 /l to 312x109 /l and heparin showed platelet count ranging from 29x10 9 /l to 210x109 /l. The mean platelet count in EDTA- anticoagulated blood of individuals with pseudothrombocytopenia was 104x109/l whereas the mean platelet count in citrate and heparin-anticoagulated samples was 151x109/land123x109/l respectively. Platelet counts decreased dramatically in the EDTA samples in contrast to the samples anticoagulated with citrate or heparin post four hours of collection.Conclusion: Peripheral blood smears should be examined for platelet clumping/aggregates in cases with low platelet count not correlating with clinical presentation or in isolated thrombocytopenia flagged in hematology analyser. Alternative anticoagulants should be used for correct estimation of platelet count.DOI: http://dx.doi.org/10.3126/jpn.v4i8.11498 Journal of Pathology of Nepal; Vol.4,No. 8 (2014) 626-629

scholarly journals Comparison of platelet count by manual and automated method

2020 ◽  
Vol 8 (10) ◽  
pp. 3523
Author(s):  
Dileep Kumar Jain
Keyword(s):  
Platelet Count ◽  
Peripheral Blood ◽  
Ethylenediaminetetraacetic Acid ◽  
Peripheral Blood Smear ◽  
Medical Sciences ◽  
Automated Method ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Automated Cell Counter ◽  
Cell Counter

Background: Since the emergence of dengue fever in the past few years, platelet count has become a routine test in every pathology lab. Common methods are by peripheral blood smears made from blood collected in ethylenediaminetetraacetic acid (EDTA) tubes, by neubaeur chamber, automated method by hematology cell counter.Methods: Blood samples of 460 adult patients and 72 children (<15 years), including indoor and outdoor, between May to August 2019, attending Hind institute of medical sciences, were collected in EDTA tubes. Samples were properly mixed on blood shaker and immediately peripheral blood smears were made and stained with Leishman stain. Platelet count of every sample was done by peripheral blood smear and by Mindray (BC5150) automated cell counter, simultaneously.Results:  Results by manual slide method are slightly higher than automated method but significantly not different from automated method.Conclusions: Traditional slide method can also be used if done carefully comparable to automated method especially useful in small labs which can’t afford automated cell counter.

scholarly journals A Preliminary Study of the Suitability of Archival Bone Marrow and Peripheral Blood Smears for Diagnosis of CML Using FISH

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Alice Charwudzi ◽  
Edeghonghon E. Olayemi ◽  
Ivy Ekem ◽  
Olufunmilayo Olopade ◽  
Mariann Coyle ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Molecular Diagnosis ◽  
Peripheral Blood ◽  
Peripheral Blood Smear ◽  
Full Blood Count ◽  
Interphase Fish ◽  
Genetic Abnormalities ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Cytogenetic Technique

Background.FISH is a molecular cytogenetic technique enabling rapid detection of genetic abnormalities. Facilities that can run fresh/wet samples for molecular diagnosis and monitoring of neoplastic disorders are not readily available in Ghana and other neighbouring countries. This study aims to demonstrate that interphase FISH can successfully be applied to archival methanol-fixed bone marrow and peripheral blood smear slides transported to a more equipped facility for molecular diagnosis of CML.Methods.Interphase FISH was performed on 22 archival methanol-fixed marrow (BM) and 3 peripheral blood (PB) smear slides obtained at diagnosis. The BM smears included 20 CML and 2 CMML cases diagnosed by morphology; the 3 PB smears were from 3 of the CML patients at the time of diagnosis. Six cases had knownBCR-ABLfusion results at diagnosis by RQ-PCR. Full blood count reports at diagnosis were also retrieved.Result.19 (95%) of the CML marrow smears demonstrated theBCR-ABLtranslocation. There was a significant correlation between theBCR-ABLtranscript detected at diagnosis by RQ-PCR and that retrospectively detected by FISH from the aged BM smears at diagnosis (r=0.870;P=0.035).Conclusion.Archival methanol-fixed marrow and peripheral blood smears can be used to detect theBCR-ABLtranscript for CML diagnosis.

scholarly journals Autosplenectomy in a Patient with Paroxysmal Nocturnal Hemoglobinuria (PNH)

2019 ◽  
Vol 2019 ◽  
pp. 1-5 ◽  
Author(s):  
Ethan Burns ◽  
Kartik Anand ◽  
Gonzalo Acosta ◽  
Malcolm Irani ◽  
Betty Chung ◽  
...  
Keyword(s):  
Hemolytic Anemia ◽  
Peripheral Blood ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Peripheral Blood Smear ◽  
Target Cells ◽  
Abdominal Ultrasonography ◽  
Blood Smears ◽  
Spleen Scintigraphy ◽  
Splenic Uptake ◽  
Peripheral Blood Smears

Autosplenectomy (AS) is a known complication of diseases such as sickle cell anemia, celiac disease, and inflammatory bowel disease. We report the first known case of AS due to paroxysmal nocturnal hemoglobinuria (PNH). A 24-year-old Caucasian male had evidence of hemolytic anemia at the age of 14 and was diagnosed with PNH at the age of 16. He had recurrent episodes of sepsis due to dialysis line infections from poor hygiene, and blood cultures had been positive for multiple organisms includingStaphylococcus aureus,Enterococcus faecalis, andStreptococcus pneumoniae. The patient’s peripheral blood smears since the age of 14 years demonstrated Howell–Jolly bodies in conjunction with thrombocytopenia and hemolytic anemia, but abdominal ultrasonography reported a normal appearing spleen. The patient presented with septicemia two years after starting eculizumab, and his peripheral blood smear showed extensive Howell–Jolly bodies, Pappenheimer bodies, acanthocytes, and target cells. Splenic ultrasonography demonstrated an atrophic spleen with multifocal scarring, and absent splenic uptake of liver-spleen scintigraphy, consistent with AS. Clinicians should remain vigilant of the potential sequelae of PNH and consider the possibility of the development of AS.

Rapid and Definitive Diagnosis of Infectious Diseases Using Peripheral Blood Smears

1992 ◽  
Vol 7 (1) ◽  
pp. 36-47 ◽  
Author(s):  
Lisa Soleymani Lehmann ◽  
Jerry L. Spivak
Keyword(s):  
Septic Shock ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Rapid Diagnosis ◽  
Definitive Diagnosis ◽  
Parasitic Infections ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Time Of Admission

A timely diagnosis is essential in the management of septicemia and septic shock. Three patients are described, all of whom presented with fever and one of whom was hypotensive at the time of admission. In each patient, rapid diagnosis of the cause of fever was possible because microorganisms were identified on a peripheral blood smear obtained at the time of admission. This identification permitted prompt initiation of appropriate antimicrobial therapy. In addition, a literature review of use of peripheral blood smears in the diagnosis of bacterial, fungal, and parasitic infections is provided.

scholarly journals Investigations of significance of blood smear results in diagnostics of infectious and parasitic diseases in dogs

2010 ◽  
Vol 64 (5-6) ◽  
pp. 375-384
Author(s):  
Aleksandar Potkonjak ◽  
Branislav Lako ◽  
Branislava Belic ◽  
Nikolina Milosevic ◽  
Ognjen Stevancevic ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Peripheral Blood ◽  
Blood Smear ◽  
Peripheral Blood Smear ◽  
Parasitic Diseases ◽  
Neutrophil Granulocyte ◽  
Inexpensive Method ◽  
Blood Smears ◽  
Dog Blood ◽  
Peripheral Blood Smears

The microscopic examination of stained smears of peripheral blood is of vital significance in the speedy diagnostics of infectious and parasitic diseases, in particular during the stage of infection when the cause is present in the blood, or blood cells. It is sometimes possible to make a definitive diagnosis of an infectious or parasitic disease following an examination of a stained smear of the peripheral blood. Since microscopic examinations of a peripheral blood smear are applied increasingly rarely in clinical practice, due to the development of other methods for the diagnostics of infectious and parasitic diseases in dogs, as well as the lack of knowledge of the morphology of the numerous causes that can be present in the blood, we carried out an investigation into the presence and spread of infections whose causes can be present in dog blood. The investigations covered 100 dogs from which peripheral blood smears were taken and then stained with a Giemsa solution according to the standard protocol and examined under a microscope with an immersion lens. The examination of peripheral blood smears stained according to Giemsa resulted in the identification of the presence of an Ehrlichia spp. morula in a neutrophil granulocyte in one dog. The presence of hemotropic mycoplasmas was established in erythrocytes of eleven dogs, while the presence of the protozoa Babesia canis in erythrocytes was identified in five dogs included in the investigations. A microscopic examination of dog peripheral blood smears stained according to Giemsa was shown as a speedy, practical, simple, and inexpensive method for making a definitive etiological diagnosis of these infections, and it should be included regularly in standard protocols for the diagnostics of infectious and parasitic diseases.

scholarly journals CLINICAL ASSESSMENT OF PANCYTOPENIA IN PERIPHERAL BLOOD SMEARS IDENTIFIED IN PATIENTS FROM BIHAR REGION

2019 ◽  
Vol 3 (8) ◽  
Author(s):  
Dr. Vivek Kumar ◽  
Dr. Jaideo Prasad
Keyword(s):  
Bone Marrow ◽  
Peripheral Blood ◽  
Megaloblastic Anemia ◽  
Bone Marrow Aspiration ◽  
Peripheral Blood Smear ◽  
Bone Marrow Examination ◽  
Peripheral Smear ◽  
Blood Smears ◽  
Function Test ◽  
Peripheral Blood Smears

The severity of pancytopenia and the underlying pathology determine the management and prognosis. [3] Thus, identification of exact cause will help in implementing appropriate therapy. The major diagnostic problems occur when there are no specific features in the peripheral smear to point the cause. In India the causes of pancytopenia are not well defined, so the present study has been undertaken to evaluate the various causes and to correlate the peripheral blood smear findings. The present study was planned in Department of Pathology, Anugrah Narayan Magadh Medical College, Gaya, Bihar from july 2017 to Dec 2017. Total 50 cases of the clinical suspicion of a hematological disorder and demonstrating pancytopenia in the peripheral blood smears were enrolled in the present study. All participants underwent a detailed history, clinical examination and investigations which included complete blood picture with red cell indices and peripheral smear, liver function test, renal function test, ultrasound abdomen and bone marrow examination in all cases. Cause of pancytopenia was ascertained and data was analysed on SPSS on the basis of etiology, clinical and haematological findings. The data generated from the present study concludes that systematic and thorough workup is required in patients presenting with pancytopenia, so that elimination of the cause is needed to treat the condition. Among them, megaloblastic anaemia and infections are early treatable and reversible. Keywords: Pancytopenia, Bone Marrow Aspiration, Megaloblastic Anemia, Hypo plastic Marrow, etc

Brightfield and fluorescence in-channel staining of thin blood smears generated in a pumpless microfluidic

2021 ◽  
Author(s):  
Kokou S. Dogbevi ◽  
Paul Gordon ◽  
Kimberly L. Branan ◽  
Bryan Khai D. Ngo ◽  
Kevin B. Kiefer ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Smears ◽  
Peripheral Blood Smears ◽  
Thin Blood Smears

Effective staining of peripheral blood smears which enhances the contrast of intracellular components and biomarkers is essential for the accurate characterization, diagnosis, and monitoring of various diseases such as malaria.

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