scholarly journals Derivative spectrophotometric determination of the herbicides picloram and triclopyr in mixtures

2007 ◽  
Vol 72 (8-9) ◽  
pp. 809-819 ◽  
Author(s):  
Biljana Abramovic ◽  
Vesna Anderluh ◽  
Ferenc Gaál ◽  
Daniela Sojic
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Second Derivative ◽  
Deconvolution Method ◽  
First Derivative ◽  
Pesticide Formulations ◽  
Influence Of Ph

A derivative spectrophotometric method for the determination of the herbicides picloram (4-amino-3,5,6-trichloropicolinic acid) and triclopyr (3,5,6-trichloro- 2-pyridyloxyacetic acid) in mixtures was developed in this work. Derivative spectrophotometric determination of the selected herbicides was preceded by investigations concerning the influence of pH, in the pH interval from 1 to 9. At pH 3.2, picloram and triclopyr solutions are stable under daylight for nine months. This pH was used for all subsequent determinations. It was also found that the use of the first derivative of the spectra at 232 nm was optimal for the determination of picloram, while use of the second derivative of the spectra at 211 nm was best suited for the determination of triclopyr. The calibration curves are linear in the concentration range 0.8 - 13 ?g cm-3 with correlation coefficients -0.9998 for picloram and 0.9996 for triclopyr. The limit of detection of the developed method is 0.08 ?g cm-3 for picloram and 0.03 ?g cm-3 for triclopyr. Derivative spectrophotometry was shown to be an appropriate method for the determination of picloram and triclopyr in mixtures and in pesticide formulations, unlike the deconvolution method. .

scholarly journals Analytical Spectrometric Study For Determining Dapagliflozin Propanediol Monohydrate Individually Or In Presence Of Metformin Hydrochloride In Tablets Formulation

2020 ◽  
Vol 17 ◽  
pp. 80-87
Author(s):  
SAAD ANTAKLI ◽  
Raghad Kabbani ◽  
And Rama Labban
Keyword(s):  
Regression Analysis ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Metformin Hydrochloride ◽  
Spectrometric Study ◽  
Limit Of Quantification ◽  
First Derivative ◽  
Linearity Range

First simple spectrophotometric method was developed and applied to determine Dapagliflozin Propanediol  Monohydrate by Zero Spectrophotometry and First Derivative Spectrophotometric method for determining of Dapagliflozin Propanediol Monohydrate (DAPA) in the presence of Metformin Hydrochloride (MET). Zero spectrophotometric (ZS) was applied for the determination of (DAPA) at 223.5 nm. Linearity range was (2.61– 31.23) µg/mL. Regression analysis showed a good correlation coefficients R2 = 0.9989. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.569 µg/mL and 1.724 µg/mL, respectively. Derivative spectrophotometric (1DS) was applied for the determination of (DAPA) in the presence (MET). (DAPA) was determined at 233 nm (1D233).  Linearity ranges were (5.21 – 41.64) µg/mL for (DAPA). Regression analysis showed a good correlation coefficients R2 = 0.9994. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.732 µg/mL and 2.218 µg/mL for (DAPA). The proposed Zero spectrophotometry method was applied to analysis individual (DAPA), and the derivative (1D233) method was applied to analysis (DAPA) individually or with (MET) combination in Syrian trademark drugs.

scholarly journals Determination of Glibenclamide By Analytical Spectrophotometry

2021 ◽  
Vol 18 ◽  
pp. 40-48
Author(s):  
Saad Antakli ◽  
Leon Nejem ◽  
Monzer Alraii
Keyword(s):  
Quality Control ◽  
Regression Analysis ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Extraction Process ◽  
Metformin Hydrochloride ◽  
Limit Of Quantification ◽  
First Derivative

Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively.  First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R2 = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB). The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (1D317) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.

scholarly journals DETERMINATION OF TRACE NITRITE AS 4-(4-NITROBENZENAZO)- 1-AMINONAPHTHALENE COMPLEX BY EXTRACTION-SPECTROPHOTOMETRY

2010 ◽  
Vol 9 (2) ◽  
pp. 254-260
Author(s):  
Choirul Amri ◽  
Dwi Siswanta ◽  
Mudasir Mudasir
Keyword(s):  
Detection Limit ◽  
Organic Solvents ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Absorbance Unit ◽  
Extraction Spectrophotometry ◽  
Linear Concentration

A study of extraction-spectrophotometric method for the determination of trace nitrite as 4-(4-nitrobenzenazo)-1-aminonaphthalene complex using n-amylalcohol and chloroform as organic solvents has been done. Results of the study showed that extraction-spectrophotometric determination of nitrite using n-amylalcohol or chloroform was very sensitive and had low limit of detection. Extraction-spectrophotometric method of nitrite using n-amylalcohol gave range of linear concentration 0.000-0.054 mg/L NO2--N, detection limit of 2.09x10-4 mg/L NO2--N, and sensitivity of 34.514 ± 0.398 absorbance unit per mg/L of NO2--N. Meanwhile, extraction-spectrophotometric of nitrite using chloroform had range of linear concentration of 0.000-0.100 mg/L NO2--N, detection limit of 8.99x10-4 mg/L NO2--N, and sensitivity of 18.353 ± 0.456 absorbance unit per mg/L NO2--N.   Keywords: Nitrite Trace, 4-(4-Nitrobenzenazo)-1-Aminonaphthalene, Extraction-Spectrophotometry

scholarly journals Spectrophotometric determination of neomycin sulphate in tablets form via reaction with ninhydrin reagent

2019 ◽  
Vol 10 (2) ◽  
pp. 1392-1396 ◽  
Author(s):  
Khalaf F Alsamarrai ◽  
Menaa Abdulsalam Al-Abbasi ◽  
Eman Thiab Alsamarrai
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Basic Medium ◽  
Optimal Conditions ◽  
Limit Of Quantification ◽  
Maximum Absorbance ◽  
Ninhydrin Reagent

A new, sensitive, simple and cheap spectrophotometric method for the determination of Neomycin Sulphate (NEO) in pharmaceutical forms has been developed. The method is based on the reaction between NEO and NIN in basic medium. The maximum absorbance was at 574 nm. The conditions affecting the reaction were optimized. Under the optimal conditions, the calibration curve was linear over the range of 0.0002-0.0011 mol/L. The limit of detection and limit of quantification were 5.423×10-6 mol/L, and 1.643×10-5 mol/L, RSD% of seven replicate was 0.8217- 0.8321% and Rec% was between 99.2168-100.8857%. The proposed method was successfully applied to the determination of NEO tablets form.

scholarly journals A novel stability-indicating method for determination of a new antidepressant effect of vortioxetine in a pharmaceutical formulation by using RP-HPLC

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Gizem Tiris ◽  
Cansu Alver ◽  
Nevin Erk
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Internal Standard ◽  
Chemical Oxidation ◽  
Hplc Method ◽  
Dihydrogen Phosphate ◽  
Stability Indicating ◽  
First Derivative ◽  
Rp Hplc

Abstract Background A novel rapid, accurate, and stability-indicating reversed-phase high performance liquid chromatographic (RP-HPLC) and first derivative spectrophotometric determination were explained for the assay of vortioxetine (VRT) in bulk and pharmaceutical formulations. For RP-HPLC method, optimal separation and determination of VRT were achieved with a Waters Symmetry C18, (100 × 4.6 mm, 3.5 μm) analytical column using a mobile phase consisting of methanol:0.05 M potassium dihydrogen phosphate (pH:3.0 ± 0.05) (30:70, v/v) in isocratic mode with flow rate of 1.3 mL min−1. Injection volume was 20 μL. The maximum absorption wavelength of VRT is 225.0 nm; hence, 225.0 nm was studied as the detection wavelength and column at 50 °C temperature. The caffeine was used as the internal standard (IS). On the other hand, the first derivative spectrophotometric method for the analysis of vortioxetine was performed by measuring the amplitude at 251.7 and 272.6 nm. Result The HPLC method was found to be linear in the concentration ranges of 10.0-70.0 μg mL−1 with the coefficient value R2 of 0.9998, and the mean recovery value was 100.7%. Further stability studies were done through exposure of the analyte solutions to various stress conditions: acid, alkali hydrolysis, chemical oxidation, and exposure to UV radiation. For the first derivative spectrophotometric method, linearity was observed in the concentration range 6.0-30.0 μg mL−1 (for 237.7 nm R2 = 0.9999 and for 257.2 nm R2 = 0.9997). Conclusion The methods were validated in accordance with ICH guidelines with respect to linearity, accuracy, specificity, limit of detection, precision, and limit of quantification.

scholarly journals Simultaneous Determination Of Atenolol And Hydrocholrothiazide In Tablets Formulation By Derivative Spectrometry

2020 ◽  
Vol 17 ◽  
pp. 73-79
Author(s):  
SAAD ANTAKLI ◽  
LEON NEJEM ◽  
MOUSTAFA ALABO JOUMAA
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Correlation Coefficients ◽  
First Derivative ◽  
Derivative Spectrometry

The derivative spectrophotometric method was developed and applied for the simultaneous determination of Atenolol (ATE) and Hydrochlorothiazide (HCT) in Tablets formulations. The first derivative spectrophotometric (1DS) method was applied for the determination of (ATE) and (HCT), respectively. (ATE) was determined at 271.9 nm (1D 271.9) and (HCT) was determined at 279.3 nm (1D 279.3). Linearity showed a good correlation coefficients R2 = 0.9994 and R2 = 0.9989 for (ATE) and (HCT), respectively. Linearity ranges were (10 – 280)

scholarly journals Development and Validation of a UV-Spectrophotometric Method for Determination of Vildagliptin and Linagliptin in Bulk and Pharmaceutical Dosage Forms

2015 ◽  
Vol 18 (2) ◽  
pp. 163-168 ◽  
Author(s):  
Sujan Banik ◽  
Palash Karmakar ◽  
Md Anowar Hossain Miah
Keyword(s):  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Pharmaceutical Journal ◽  
Dosage Forms ◽  
Limit Of Quantification ◽  
Allowable Limit ◽  
Pharmaceutical Dosage Forms ◽  
Tablet Dosage

The present study was undertaken to develop a spectrophotometric method for determination of vildagliptin and Linagliptin in pharmaceutical dosage forms. This paper describes a simple, rapid, accurate and precise UVspectrophotometric method for the assay of vildagliptin and linagliptin in bulk and marketed tablet dosage forms. The validation of the developed method was carried out according to ICH guidelines with respect to linearity, precision, accuracy, specificity, limit of detection and limit of quantification. Calibration curves were obtained in the concentration range of 8-32 ?g/ml for vildagliptin and 5-25 ?g/ml for linagliptin with good correlation coefficients (r=0.999). The precisions of the new method for both drugs were less than the maximum allowable limit (%RSD < 2.0) specified by the USP, ICH and FDA. Therefore, the method was found to be an accurate, reproducible and sensitive for analysis of vildagliptin and linagliptin in pharmaceutical dosage forms.Bangladesh Pharmaceutical Journal 18(2): 163-168, 2015

scholarly journals Spectrophotometric determination of Valsartan in pure form and in its pharmaceutical preparations

2021 ◽  
Vol 26 (4) ◽  
Author(s):  
Qabas Rashid ◽  
Ruwaida Farman Salih
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Pure Form ◽  
Chromogenic Reagent

An easy, rapid and economical spectrophotometric method for  determination of  Valsartan (Val), by reaction with 4-chloro-7-nitrobenzofurazan (NBD-Cl) as reagent in an alkaline interemediate. This method is based on the forming of product between (Val) and the chromogenic reagent (NBD-Cl), to produce a brown color at (pH 11.9) and λmax. 470 nm.  Beer’s Law is obeyed at the concentrations range of (0.4-14.8 µg/ml), with molar absorptivity of (1.05×104 L/mol.cm) and correlation coefficient 0.9827, The limit of detection was 0.557 µg/ml. The suggested method was prosperity implement to the determination of (Val) in  pure form and in its pharmaceutical formulations (tablets).

A New Second-Derivative Spectrophotometric Method for the Determination of Vildagliptin in Pharmaceutical dosage form

2021 ◽  
Vol 12 (4) ◽  
pp. 2610-2614
Author(s):  
Subhadip C ◽  
Nalanda Baby R ◽  
Pridhvi Krishna G ◽  
Suraj M ◽  
Shyamdeo Kumar T
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Standard Addition ◽  
Second Derivative ◽  
Limit Of Quantification ◽  
Precision Data ◽  
Pharmaceutical Dosage Form ◽  
Relative Standard

An accurate derivative spectrophotometric method was developed and validated for the determination of dipeptidyl peptidase inhibitor vildagliptin in the pharmaceutical dosage form. The second derivative of the UV spectra has enabled the estimation of vildagliptin absorbance at 217 nm without any interferences. Linearity, precision, accuracy, detection (LOD), and quantification (LOQ) limits were established for method validation. Calibration curve was linear in the range of 10-60 µg/mL with a regression coefficient of 0.998. The method was validated as per the International Conference on Harmonization (ICH Q2 (R1)). The limit of detection and the limit of quantification were found to be 2.06 µg/mL and 6.25 µg/mL, respectively. Intra and interday precision data illustrated that the method has acceptable reproducibility as the percentage relative standard deviation (RSD) was less than 2 %, which indicates the precision of the method. The recovery was 98.39 % by the standard addition method. The percentage assay of vildagliptin was 98.06 % which showed good applicability. The following results indicate that the procedure is accurate, precise, and reproducible while being simple and less time-consuming. The method was demonstrated to be adequate for routine analysis in quality control. 

Collaborative Study of the Spectrophotometric Determination of Procainamide Hydrochloride

1976 ◽  
Vol 59 (4) ◽  
pp. 807-810
Author(s):  
Jeffrey C Hamm
Keyword(s):  
Sodium Hydroxide ◽  
Acid Medium ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Collaborative Study ◽  
Dosage Forms ◽  
Standard Deviations

Abstract The USP analysis for procainamide HCl is titrimetric and relatively nonspecific, capsule and tablet dyes may interfere, and the method is not applicable to coated tablets. In the spectrophotofluorometric method the sample deteriorates when exposed to a xenon source. In the ultraviolet spectrophotometric method reported here, the sample is dispersed in acid medium, possible interferences are extracted in chloroform, base is added, procainamide is extracted in chloroform, the residue is dissolved in sodium hydroxide, and the compound is measured by absorption at 272 nm and comparison with a standard. Recoveries of standards added to capsule, tablet, and injection composites ranged from 99.3 to 102%. Twelve collaborators reported duplicate assay results for all 3 dosage forms with per cent standard deviations for 5 samples ranging from 1.01 to 1.27%. The method has been adopted as official first action.

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