Response of in vitro propagated fig (Ficus carica L.) shoots to the concentrations of benzyl amino purine and coconut water

Abstract In this study, several concentrations of benzyl amino purine (BAP) and coconut water (CW) were investigated along with the interaction between two factors to the growth of in vitro propagated fig shoots. The investigated factors consisted of BAP concentration: 0, 1, 3, 5 mg L−1 and coconut water concentration: 0, 100, 200, 300 ml L−1. A total of 16 treatment combinations with 6 replications resulting in 96 experimental units consisting of a single fig shoot explant per culture medium. The observed parameters including living explant rate, contamination rate, browning rate, day of first shoot emergence, shoot formation rate, explant height addition, number of leaves, callus formation rate, and number of roots were conducted every week from 1 to 8 weeks after proliferation (WAP). The result indicated that in 8 WAP, the living explant rate reached 23.95%. The combination of concentration 200 ml L−1 CW and 3 mg L−1 BAP + 200 ml L−1 CW-induced early emergence of new shoots at 7 days after proliferation (DAP). The highest shoot formation rate (100%) was observed at a concentration of 300 mL L−1CW. The highest explant height addition (7.10 cm) was observed at a concentration of 200 mL L−1 CW. The highest number of leaves (5.80) was observed at a concentration of 1 mg L−1 BAP + 200 mL L−1 CW. The highest callus formation rate (50%) was observed at a concentration of 100 ml L−1CW and 300 ml L−1 CW. The highest number of roots (17) was observed in the control.

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Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

Jurnal SAINS ALAMI (Known Nature) ◽

10.33474/j.sa.v3i2.7222 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Wulan Dari Neng Gumiwang ◽

Tintrim Rahayu ◽

Ari Hayati

Keyword(s):

Root Length ◽

Cocos Nucifera ◽

Water Concentration ◽

Coconut Water ◽

Culture Bottle ◽

Randomized Design ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%) dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan

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PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

AGRI-SOSIOEKONOMI ◽

10.35791/agrsosek.14.1.2018.19730 ◽

2018 ◽

Vol 14 (1) ◽

pp. 439

Author(s):

Alfrida ., Maninggolang ◽

Jeany Sh. Polii-Mandang ◽

Wenny ., Tilaar

Keyword(s):

Brassica Oleracea ◽

Coconut Water ◽

Shoot Bud ◽

Randomized Design ◽

Wet Weight ◽

Number Of Leaves ◽

Brassica Oleracea L ◽

Benzyl Amino Purine ◽

Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

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THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽

10.31315/agrivet.v27i1.4694 ◽

2021 ◽

Vol 27 (1) ◽

pp. 39

Author(s):

Salma Nabila ◽

Endah Budi Irawati ◽

Rina Srilestari

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Fresh Weight ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design ◽

Range Test ◽

Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

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Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis ◽

10.11118/actaun201967010009 ◽

2019 ◽

Vol 67 (1) ◽

pp. 9-18

Author(s):

Enik Akhiriana ◽

Samanhudi ◽

Ahmad Yunus

Keyword(s):

Water Concentration ◽

Coconut Water ◽

Tribulus Terrestris ◽

Emergence Time ◽

Shoot Height ◽

Randomized Design ◽

Number Of Leaves ◽

Indole 3 Acetic Acid ◽

Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

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Determination of the Effect of Magnesium Oxide Nanoparticles (MgO-NP) on in Vitro Culture of Cowpea (Vigna Unguiculata L. Walp)

10.21203/rs.3.rs-1088469/v1 ◽

2021 ◽

Author(s):

Rabia KOÇAK ◽

Melih OKCU ◽

Kamil Haliloglu ◽

Azize Alayli ◽

Hayrunnisa Nadaroglu

Keyword(s):

Callus Formation ◽

Formation Rate ◽

Synthesis Method ◽

Shoot Formation ◽

Walnut Shell ◽

Mgo Nanoparticles ◽

Mean Values ◽

Shoot Number ◽

Different Doses

Abstract This study was carried out to determine the effect of different doses of MgO nanoparticles (MgO-NP's) on in vitro regeneration of cowpea. MgO NPs used in the study were synthesized using walnut shell extract by green synthesis method. MgO nanoparticles with 35-40 nm size were used in this research. In the study, the effect of different doses of MgO NP applied to cowpea plant on all in vitro parameters was found to be significant. Considering the parameters examined, the best results in morphogenesis were 185 mg/L, 370 mg/L and 555 mg/L MgO-NPs applications, the highest shoot formation rate was obtained 82.50% with 555 mg/L(MgO-NPs) and 72.50% with 370 mg/L (MgO-NPs). The highest mean values of shoot number, shoot number per explant and shoot length were observed in the application of 370 mg/L MgO-NPs with 61.25, 17.50, and 2.075 (unit) respectively. The best root formation rate was obtained from control and 370 mg/L MgO application at a rate of 27.50%. The highest values were 6.75 (pieces) and 370 mg/L MgO-NPs in the number of roots per explant, 555 mg/L MgO-NPs with 10% in callus formation, and 1.575 (cm) and 370 mg/L MgO-NPs in root length. When all the examined parameters were evaluated, it was determined that the application of 370 mg/L MgO-NPs gave the best results in terms of in vitro parameters.

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Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽

10.30598/a.v1i1.293 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

S. Tuhuteru ◽

Meity L Hehanussa ◽

Simon H.T Raharjo

Keyword(s):

Culture Medium ◽

Culture Media ◽

Water Concentration ◽

Medium Composition ◽

Coconut Water ◽

Orchid Species ◽

Randomized Design ◽

Wet Weight ◽

Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media. The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids. Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

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The effect of NAA and coconut water combination on garlic (Allium sativum L.) tissue culture

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/905/1/012036 ◽

2021 ◽

Vol 905 (1) ◽

pp. 012036

Author(s):

Nandariyah ◽

L S Mahmudah ◽

R B Arniputri ◽

A T Sakya

Keyword(s):

Tissue Culture ◽

Coconut Water ◽

Emergence Time ◽

Shoot Height ◽

Culture Techniques ◽

Randomized Design ◽

Two Factors ◽

Number Of Leaves ◽

Tissue Culture Techniques ◽

Number Of Shoots

Abstract Tissue culture techniques can increase the number of garlic seedlings. The purpose of this research is to determine the effect of NAA and coconut water in increasing the number of garlic seeds. This research used a Completely Randomized Design of two factors. The treatment used is NAA with concentrations of 0 ppm, 0.5 ppm, 1 ppm, 1.5 ppm, and coconut water concentrations of 0%, 10%, 20%. The variables observed were shoot emergence time, root emergence time, number of shoots, number of roots, number of leaves, shoot height, root length, and number of plantlets. The results showed that the addition of coconut water 20% without the addition of NAA in 1 bulb can produce 3.33 planlets and the results of explant propagation in 1 bulb can produce the number of shoots as many as 15.33 shoots. Giving coconut water with concentrations of 10% and 20% can increase the number of leaves, shoot height, and some planlets. The concentration of NAA 0.5 ppm can accelerate the root emergence time on garlic explant.

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Immature embryo is the potential source for in vitro plant regeneration in Jatropha curcas

Journal of Bio-Science ◽

10.3329/jbs.v20i0.17726 ◽

2014 ◽

Vol 20 ◽

pp. 125-134

Author(s):

MR Islam ◽

MA Bari

Keyword(s):

Developmental Stages ◽

Callus Formation ◽

Immature Embryo ◽

Basal Medium ◽

Coconut Water ◽

Immature Embryos ◽

Plantlet Formation ◽

Potential Source ◽

Wide Range

Context: Jatropha belongs the spurge family Euphorbiaceae. Special interest mounting for its biodiesel which has created enthusiasm in cultivation of the species for oil extraction. Objectives: The study was conducted to develop the protocol for tissue and callus culture in Bangladeshi Jatropha curcus plant particularly to identify the most suitable explants for its wide scale micropropagation. Materials and Methods: Immature embryos taken from four developmental stages of fruits were cultured on growth regulator free MS liquid medium. After fifteen days of germination, elongated hypocotyls and two cotyledonary leaves were used as explants. Results: Embryo derived seedlings acted as the potential source of explants both for callus and plantlets. The immature embryo of size 0.87cm produced highest callus formation (83.33%) on MS medium supplemented with lower concentration of 2, 4-D (0.5 mg/l) and coconut water 2% (v/v). Immature embryos grown on MS basal medium supplemented with 2,4-D (0.2 mg/l, 0.5 mg/l and 1.0 mg/l) alone or in combination with coconut water 2% (v/v) exhibited a wide range of callus induction percentage (26-100%) for hypocotyls and (20 - 40%) for cotyledonary leaves. Conclusion: The age of immature embryo and addition of growth adjuvants and growth additive to the culture medium played the role in promoting better callus and plantlet formation. DOI: http://dx.doi.org/10.3329/jbs.v20i0.17726 J. bio-sci. 20: 125-134, 2012

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882 PB 276 IN VITRO PROPAGATION AND CHIMERAL TRAITS OF Cryptanthus `Marian Oppenheimer' (WIDE LEAF CLONE)

HortScience ◽

10.21273/hortsci.29.5.560c ◽

1994 ◽

Vol 29 (5) ◽

pp. 560c-560

Author(s):

Yong Cheong Koh ◽

Fred T. Davies

Keyword(s):

In Vitro Propagation ◽

Callus Formation ◽

Adventitious Shoot ◽

Shoot Formation ◽

Induction Medium ◽

Ms Medium ◽

Periclinal Chimera ◽

Adventitious Shoot Formation ◽

L1 And L2

The leaves of vegetative stolons of greenhouse grown Cryptanthus `Marian Oppenheimer' (wide leaf clone) were cultured in modified MS media to induce adventitious shoot formation via callus formation. The best callus induction medium was basal MS medium with 10 μM NAA, IBA and BA. Pure green (843), maroon (3), striped (2) and albino plantlets were obtained. Most of the albino plantlets were stunted, tightly clumped together and impossible to score. The medium which produced the highest average number of non-albino plantlets was basal MS medium with 0.3 μM NAA, IBA and BA All non-albino plantlets were rooted in MS medium with 5.4 μM NAA and transplanted ex vitro with a survival rate of 96.7%. The maroon plantlets became green two weeks after transplanting. Histological studies revealed that C. `Marian Oppenheimer' (wide leaf clone) has two tunicas (L1 and L2) and a corpus (L3). Callus on the leaf explant arose mainly from the L2 and L3. Apparently C. `Marian Oppenheimer' (wide leaf clone) is a GWG periclinal chimera.

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PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale

Caraka Tani Journal of Sustainable Agriculture ◽

10.20961/carakatani.v31i1.11938 ◽

2016 ◽

Vol 31 (1) ◽

pp. 33

Author(s):

Sri Hartati ◽

Agus Budiyono ◽

Ongko Cahyono

Keyword(s):

Plant Conservation ◽

Naphthalene Acetic Acid ◽

Randomized Design ◽

Significant Difference ◽

Two Factors ◽

Number Of Leaves ◽

Media Research ◽

Test Level ◽

Range Test

Indonesia has a high diversity of species of orchids. One of them is the Dendrobium orchid. Orchid seeds do not have endosperm should be grown in a medium that has enough nutrients. Orchid propagation in vitro is strongly influenced by the composition of the medium used. Research through experiments aimed at studying the effect of NAA, BAP, NAA and BAP combination of the subculture plantlets growth of hybrids orchid Dendrobium biggibum X liniale in Vacin Went media. Research conducted at the Laboratory of Tissue Culture Center for Plant Conservation Bogor Botanical Gardens. The experimental design used was completely randomized design (CRD) with two factors. The first factor was the concentration of Naphthalene Acetic Acid /NAA ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). The second factor was the type media of Benzyl Amino Purine /BAP ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). Each treatment was replicated eight times. Analysis of the data by F test level 5% and if there is a significant difference continued with Duncan Multiple (Duncan's Multiple Range Test) level of 5%. The result showed that the addition of NAA 3 ppm as much as 4,96 cm and BAP 3 ppm as much as 4,41 cm give a significant effect on the increase of high plantlets and so NAA 3 ppm as much as 5,76 cm effect on roots length , but did not significantly effect the number of leaves and roots.

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