Erratum to: The blood vessels development, morphogenesis and blood circulation are three ontologic groups highly up-regulated in porcine oocytes before in vitro maturation

AbstractThe mammalian oocytes undergo significant biochemical and structural modifications during maturation both in vitro and in vivo. These changes involve chromatin reorganization and modification within metabolic status of cytoplasmic organelles. After oocytes’ successful maturation the substantially increased storage of RNA was observed. Moreover, the early embryo interaction with maternal endometrial tissue after fertilization is up to now considered as the main marker of proper embryo implantation and early growth. In this study, we first investigated the expression profile of genes involved in blood vessel formation and blood circulation in porcine oocytes before and after in vitro maturation.The cumulus-oocyte complexes were collected from pubertal Landrace gilts and classified as before in vitro maturation (in Vivo) or after in vitro maturation (in Vitro). The RNA was isolated from these two experimental groups and analyzed using Affymetrix microarrays.We found an increased expression of genes involved in ontological groups such as “blood circulation” (TPM1, ECE1, ACTA2, EPHX2, EDNRA, NPR2, MYOF, TACR3, VEGFA, GUCY1B3), “blood vessel development” (ANGPTL4, CYR61, SEMA5A, ID1, RHOB, RTN4, IHH, ANGPT2, EDNRA, TGFBR3, MYO1E, MMP14), and “blood vessels morphogenesis” (ANGPT2, as well as other common transcripts) in in Vivo group as compared to decreased expression of these genes in in Vitro group of oocytes.It has been suggested that investigated genes undergo significant expression before in vitro maturation, when enhanced storage of large amount of RNA takes place. Creating templates for synthesis of proteins is required for formation of fully mature gametes and early embryo growth. Therefore we hypothesized that the processes of vascularization and/or angiogenesis reach a high activity in immature oocytes and are distinct from achievement of maturational stage by oocytes in pigs.

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Follicular fluid leptin concentrations and expression of leptin and leptin receptor in the equine ovary and in vitro-matured oocyte with reference to pubertal development and breeds

Reproduction Fertility and Development ◽

10.1071/rd12188 ◽

2013 ◽

Vol 25 (5) ◽

pp. 837 ◽

Cited By ~ 7

Author(s):

Anna Lange-Consiglio ◽

Silvana Arrighi ◽

Nadia Fiandanese ◽

Paola Pocar ◽

Marina Aralla ◽

...

Keyword(s):

Blood Vessels ◽

Follicular Fluid ◽

In Vitro Maturation ◽

Leptin Receptor ◽

Expression Profiles ◽

Pubertal Development ◽

Corpora Lutea ◽

Receptor Mrna ◽

Matured Oocyte

There is no published information about follicular-fluid leptin concentrations or the presence of leptin and leptin receptor in the equine ovary or oocyte. Three groups of mares – adult draft mares, draft fillies and adult Standardbred mares – were included in the study. Leptin and leptin receptor were detected in all immature oocytes by immunofluorescence with higher intensity in oocytes from draft mares compared with draft fillies and Standardbred mares. After in vitro maturation a higher proportion of oocytes reached metaphase II in draft mares than in draft fillies and Standardbred mares, and in all groups both leptin and leptin receptor became localised in the oocyte cortex but with higher immunopositivity in draft mares compared with draft fillies and Standardbred mares. These intensities were confirmed by the expression profiles of leptin and leptin receptor mRNA. Moreover, leptin was detected in ovarian blood vessels in all three types of animal and within the corpora lutea in adult mares. Serum and follicular-fluid concentrations of leptin were similar in draft and Standardbred mares but higher in draft mares than in draft fillies. This study supports the hypothesis that expression of leptin and leptin receptor mRNA and the rate of maturation can be related either to adiposity or to puberty.

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In vitro maturation of primate oocytes retrieved at the time of cesarean section

Journal of the Society for Gynecologic Investigation ◽

10.1016/s1071-5576(97)86351-4 ◽

1998 ◽

Vol 5 (1) ◽

pp. 106A-106A

Author(s):

R BRZYSKI ◽

M LELAND ◽

C EDDY

Keyword(s):

Cesarean Section ◽

In Vitro Maturation

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Effect of isoflavone compounds on the in vitro maturation of sheep oocytes

Planta Medica ◽

10.1055/s-0035-1565604 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

AA Amir ◽

GB Martin ◽

JM Kelly ◽

DO Kleemann ◽

Z Durmic ◽

...

Keyword(s):

In Vitro Maturation

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Persistence of Fibrinolytic Activity in Fragments of Human Veins Cultured in Vitro

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654209 ◽

1970 ◽

Vol 24 (01/02) ◽

pp. 043-047 ◽

Cited By ~ 4

Author(s):

M Pandolfi

Keyword(s):

Blood Vessels ◽

Plasminogen Activator ◽

Fibrinolytic Activity ◽

Vasa Vasorum ◽

Adult Human ◽

Slide Technique

SummaryExplants from 5 adult human veins were cultured in a fibrinolytically inactive medium for 3 weeks and assayed for the presence of plasminogen activator by the fibrin slide technique. The explants from 3 veins showed fibrinolytic activity confined to their vasa vasorum for the whole duration of the culture; no decrease of activity was seen. The finding suggests that small blood vessels are able to synthesize plasminogen activator.

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