scholarly journals Simultaneous Determination of Atorvastatin and Amlodipine in Industrial Tablets by Apparent Content Curve and HPLC Methods

2013 ◽  
Vol 59 (1) ◽  
pp. 44-48
Author(s):  
Silvia Imre ◽  
Klára Kacsó ◽  
Daniela-Lucia Muntean
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Reference Method ◽  
Hplc Method ◽  
Test Method ◽  
Fixed Dose ◽  
Mathematical Approach ◽  
Curve Method ◽  
The Difference

AbstractIntroduction: This study proposes the simultaneous determination of atorvastatin and amlodipine in industrial tablets by a quantitative spectrophotometric method, named the apparent content curve method, test method, and by an HPLC method with UV detection as reference method.Materials and methods: A synthetic mixture and two fixed medicinal combinations containing amlodipine and atorvastatin were investigated by the apparent content curve method, a simple and relatively inexpensive UV-VIS spectrophotometric method based on a mathematical approach derived from the Lambert-Beer law. The results were compared with those obtained by an HPLC method.Results: A good correlation of the results was obtained, the difference between the pair results was not significant (p >0.05).Conclusions: The proposed spectrophotometric method is an easier and cheaper alternative for the quantitative determination of amlodipine and atorvastatin in industrial fixed-dose combinations.

NOVEL UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF SITAGLIPTIN PHOSPHATE AND SIMVASTATIN BY AREA UNDER CURVE METHOD

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (04) ◽  
pp. 46-52
Author(s):  
V. V. Chopde ◽  
◽  
P. M. Patil ◽  
S. D Rathod ◽  
P. D. Chaudhari
Keyword(s):  
Simultaneous Determination ◽  
Wavelength Range ◽  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Economic Method ◽  
Curve Method ◽  
Dose Combination

A simple, versatile, accurate, precise and economic method for simultaneous determination of sitagliptin phosphate and simvastatin in fixed dose combination products was developed. The absorbance values at 267 nm and 239 nm for sitagliptin phosphate and simvastatin. The combination is also estimated by AUC method it involved measurement of area under curve in the wavelength range is 264-270 nm (λ1 -λ2 ) and 236-242 nm (λ3 -λ4 ) sitagliptin phosphate and simvastatin respectively. This method obeyed Beer’s law in the concentration range of 10-60 μg /mL for sitagliptin phosphate and 2-12 μg /mL for simvastatin. The results of analyses have been validated statistically for linearity, accuracy, precision, LOD and LOQ of the proposed method.

scholarly journals MULTICOMPONENT SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ANALYSIS OF DELAPRIL AND INDAPAMIDE IN TABLETS

2017 ◽  
Vol 1 (1) ◽  
pp. 50-55
Author(s):  
Vitor Todeschini ◽  
Regiane Leite ◽  
Maximiliano Sangoi ◽  
Paulo Oliveira ◽  
Thiago Barth
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Partial Least Squares Regression ◽  
Fixed Dose ◽  
Least Squares Regression ◽  
Acceptable Range ◽  
Validation Parameters ◽  
Simple Alternative ◽  
Predict Model

A multicomponent ultraviolet (UV) spectrophotometric method was developed and validated for simultaneous determination of delapril (DEL) and indapamide (IND) in tablets employing the partial least squares regression (PLSR) approach. The PLSR method was developed by a multilevel factorial design using 25 synthetic mixtures of drugs and a significant predict model (p < 0.05) were obtained at 225 nm for DEL (r2 = 0.9992) and 243 nm for IND (r2 = 0.9997). Validation parameters such as the specificity, linearity, precision, accuracy and robustness were evaluated in accordance with the ICH requirements, giving satisfactory results within the acceptable range. The proposed PLSR method was successfully applied for simultaneous determination of DEL and IND in fixed dose combinations and can be used as simple alternative to separation techniques.

scholarly journals A rapid stability-indicating, fused-core HPLC method for simultaneous determination of β-artemether and lumefantrine in anti-malarial fixed dose combination products

2013 ◽  
Vol 12 (1) ◽  
pp. 145 ◽  
Author(s):  
Sultan Suleman ◽  
Kirsten Vandercruyssen ◽  
Evelien Wynendaele ◽  
Matthias D’Hondt ◽  
Nathalie Bracke ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Stability Indicating ◽  
Dose Combination

scholarly journals The comparison of HPLC and spectrophotometric method for cholesterol determination

10.5219/1302 ◽  
2020 ◽  
Vol 14 ◽  
pp. 118-124
Author(s):  
Lukáš Kolarič ◽  
Peter Šimko
Keyword(s):  
Spectrophotometric Method ◽  
Nutritional Value ◽  
Cholesterol Content ◽  
Hplc Method ◽  
Reverse Phase ◽  
The Difference ◽  
Lower Limits ◽  
Cholesterol Determination ◽  
Higher Sensitivity

The present study was carried out to compare two different analytical methods (HPLC and spectrophotometric) for determination of cholesterol content in milk while cholesterol in food is important not only for the nutritional value setting of foods but also due to the validation of a fast, reliable and economical method for studying the possible mechanism of its reduction. Spectrophotometric determination of cholesterol content was based on the Liebermann-Burchard (LB) reaction among cholesterol, ethyl acetate, acetic anhydride, plus concentrated H2SO4 and measuring absorbance of formed color at 625 nm. HPLC method was performed by column chromatography on reverse phase C18 with DAD detection at 205 nm. The methods were applied to the milk sample. The achieved LOD and LOQ for HPLC were 2.13 mg.kg-1 and 6.45 mg.kg-1, respectively, while for spectrophotometric method were 12.55 and 38.04 mg.kg-1. The difference between cholesterol content determined by both methods was statistically insignificant at p <0.05. Therefore, it can be concluded that both methods are suitable for determination of cholesterol content in milk, however, HPLC method exhibited higher sensitivity and lower limits of detection or quantification, respectively.

scholarly journals Development and validation of an HPLC method for the simultaneous determination of artesunate and mefloquine hydrochloride in fixed-dose combination tablets

2013 ◽  
Vol 49 (4) ◽  
pp. 837-843 ◽  
Author(s):  
Fernando Henrique Andrade Nogueira ◽  
Naialy Fernandes Araújo Reis ◽  
Paula Rocha Chellini ◽  
Isabela da Costa César ◽  
Gerson Antônio Pianetti
Keyword(s):  
Simultaneous Determination ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Array Detector ◽  
Control Analysis ◽  
Dose Combination ◽  
Development And Validation

The present study developed and validated an HPLC method for the simultaneous determination of artesunate (AS) and mefloquine hydrochloride (MQ) in fixed-dose combination tablets, according to ICH guidelines. The chromatographic separation was carried out on an XBridge C18 (250 x 4.6 mm i.d., 5 µm particle size, Waters) analytical column. The mobile phase included a 0.05 M monobasic potassium phosphate buffer (pH adjusted to 3.0 with phosphoric acid) and acetonitrile (50 + 50, v/v). The flow rate was 1.0 mL/min, and the run time was 13 minutes. A dual-wavelength approach was employed: AS detection was performed at 210 nm and MQ was detected at 283 nm, using a diode array detector. Stability of sample solutions was evaluated for 8 hours after preparation, during which time the solutions remained stable. Youden's test was employed to evaluate robustness. The method proved to be linear (r²>0.99), precise (RSD<2.0%), accurate, selective, and robust, proving to be appropriate for routine drug quality control analysis.

Simultaneous Determination of Ursodeoxycholic Acid and Chenodeoxycholic Acid in Pharmaceutical Dosage Form by HPLC–UV Detection

2017 ◽  
Vol 100 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Mostafa A Khairy ◽  
Fotouh R Mansour
Keyword(s):  
Ursodeoxycholic Acid ◽  
Simultaneous Determination ◽  
Chenodeoxycholic Acid ◽  
Dosage Form ◽  
Reference Method ◽  
Reversed Phase ◽  
Hplc Method ◽  
Uv Detection ◽  
Pharmaceutical Dosage Form

Abstract A reversed-phase HPLC method was developed for the simultaneous determination of ursodeoxycholic acid (UDCA) and the epimeric isomer, chenodeoxycholic acid (CDCA), in their synthetic mixtures and in tablet dosage form. The proposed HPLC method uses a C18 column and mobile phase consisting of an acetonitrile–phosphate buffer mixture (pH 2.3, 100 mM; 50 + 50, v/v) at a flow rate of 2.0 mL/min with UV detection at 210 nm. The method was validated according to the International Conference on Harmonization guidelines; and linearity, range,accuracy, precision, robustness,and system suitability were studied. The LOD and LOQ were also calculated and found to be 1.23 and 3.73 μg/mL for UDCA and 0.83 and 2.52 μg/mL for CDCA, respectively. The method was adapted for UHPLC, in which baseline separation was achieved in &lt;2.5 min. The assay results of Ursomix tablets by the developed method were statistically compared with those obtained by the reference method using t- and F-tests, and no significant differences were observed.

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