NOVEL UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF SITAGLIPTIN PHOSPHATE AND SIMVASTATIN BY AREA UNDER CURVE METHOD

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (04) ◽  
pp. 46-52
Author(s):  
V. V. Chopde ◽  
◽  
P. M. Patil ◽  
S. D Rathod ◽  
P. D. Chaudhari
Keyword(s):  
Simultaneous Determination ◽  
Wavelength Range ◽  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Economic Method ◽  
Curve Method ◽  
Dose Combination

A simple, versatile, accurate, precise and economic method for simultaneous determination of sitagliptin phosphate and simvastatin in fixed dose combination products was developed. The absorbance values at 267 nm and 239 nm for sitagliptin phosphate and simvastatin. The combination is also estimated by AUC method it involved measurement of area under curve in the wavelength range is 264-270 nm (λ1 -λ2 ) and 236-242 nm (λ3 -λ4 ) sitagliptin phosphate and simvastatin respectively. This method obeyed Beer’s law in the concentration range of 10-60 μg /mL for sitagliptin phosphate and 2-12 μg /mL for simvastatin. The results of analyses have been validated statistically for linearity, accuracy, precision, LOD and LOQ of the proposed method.

Development and Validation of an HPLC Method for Simultaneous Determination of Rifampicin, Isoniazid, Pyrazinamide, and Ethambutol Hydrochloride in Pharmaceutical Formulations

2015 ◽  
Vol 98 (5) ◽  
pp. 1234-1239 ◽  
Author(s):  
Paula R Chellini ◽  
Eduardo B Lages ◽  
Pedro H C Franco ◽  
Fernando H A Nogueira ◽  
Isabela C César ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Combined Treatment ◽  
Pharmaceutical Formulations ◽  
Gradient Elution ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Array Detector ◽  
Equilibration Time ◽  
Dose Combination

Abstract Tuberculosis treatment consists of a fixed dose combination of rifampicin (RIF), isoniazid (INH), pyrazinamide (PYZ), and ethambutol hydrochloride (EMB). The combined treatment using various drugs is necessary for patient curing, without recrudescence, and for prevention of drug-resistant mutants, which may occur during treatment. An HPLC-diode array detector (DAD) method for the simultaneous determination of RIF, INH, PYZ, and EMB in fixed dose combination tablets was developed and validated. Chromatographic experiments were performed on an Agilent 1200 HPLC system, and the separation was carried out on a Purospher STAR RP18e (250 × 4.6 mm id, 5 μm, Merck) analytical column. Gradient elution was carried out with a mobile phase of 20 mM monobasic sodium phosphate buffer with 0.2% triethylamine (pH 7.0) and acetonitrile at a flow rate of 1.5 mL/min. The total run time was 12 min, and the re-equilibration time was 5 min. EMB detection was performed at 210 nm, and RIF, INH, and PYZ were detected at 238 nm, using a DAD. The method proved to be specific, linear (r2 > 0.99), precise (RSD <2%), accurate, and robust and may be applied to the QC analysis of pharmaceutical formulations.

Use of chemometrics to compare NIR and HPLC for the simultaneous determination of drug levels in fixed-dose combination tablets employed in tuberculosis treatment

2018 ◽  
Vol 149 ◽  
pp. 557-563 ◽  
Author(s):  
Kelly Sivocy Sampaio Teixeira ◽  
Said Gonçalves da Cruz Fonseca ◽  
Luís Carlos Brigido de Moura ◽  
Mario Luís Ribeiro de Moura ◽  
Márcia Herminia Pinheiro Borges ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Tuberculosis Treatment ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Drug Levels ◽  
Dose Combination

Development of a robust SFC method for evaluation of compatibility for a novel antituberculotic fixed-dose combination

2019 ◽  
Vol 11 (13) ◽  
pp. 1777-1787 ◽  
Author(s):  
Valentina Petruševska ◽  
Iva Krtalić ◽  
Andrea Rašić ◽  
Ana Mornar
Keyword(s):  
Simultaneous Determination ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Chromatography Method ◽  
Dose Combination

A fast and simple supercritical fluid chromatography method for the simultaneous determination of two antituberculotic drugs, isoniazid and rifabutin, and their impurities from a new proposed fixed-dose combination (FDC) was developed.

scholarly journals A rapid stability-indicating, fused-core HPLC method for simultaneous determination of β-artemether and lumefantrine in anti-malarial fixed dose combination products

2013 ◽  
Vol 12 (1) ◽  
pp. 145 ◽  
Author(s):  
Sultan Suleman ◽  
Kirsten Vandercruyssen ◽  
Evelien Wynendaele ◽  
Matthias D’Hondt ◽  
Nathalie Bracke ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Stability Indicating ◽  
Dose Combination

scholarly journals Innovative stability-indicating LC-Corona CAD method for simultaneous determination of assay in Artesunate and Mefloquine hydrochloride fixed-dose combination product

2021 ◽  
pp. 103657
Author(s):  
Wilson Camargo ◽  
Diogo Dibo ◽  
Monique Silva dos Santos ◽  
Ivone de Jesus do Nascimento Lopes ◽  
Flávia Furtado de Mendonça de Sousa ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Combination Product ◽  
Stability Indicating ◽  
Dose Combination ◽  
Corona Cad

scholarly journals Simultaneous Determination of Atorvastatin and Amlodipine in Industrial Tablets by Apparent Content Curve and HPLC Methods

2013 ◽  
Vol 59 (1) ◽  
pp. 44-48
Author(s):  
Silvia Imre ◽  
Klára Kacsó ◽  
Daniela-Lucia Muntean
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Reference Method ◽  
Hplc Method ◽  
Test Method ◽  
Fixed Dose ◽  
Mathematical Approach ◽  
Curve Method ◽  
The Difference

AbstractIntroduction: This study proposes the simultaneous determination of atorvastatin and amlodipine in industrial tablets by a quantitative spectrophotometric method, named the apparent content curve method, test method, and by an HPLC method with UV detection as reference method.Materials and methods: A synthetic mixture and two fixed medicinal combinations containing amlodipine and atorvastatin were investigated by the apparent content curve method, a simple and relatively inexpensive UV-VIS spectrophotometric method based on a mathematical approach derived from the Lambert-Beer law. The results were compared with those obtained by an HPLC method.Results: A good correlation of the results was obtained, the difference between the pair results was not significant (p >0.05).Conclusions: The proposed spectrophotometric method is an easier and cheaper alternative for the quantitative determination of amlodipine and atorvastatin in industrial fixed-dose combinations.

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