scholarly journals MULTICOMPONENT SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ANALYSIS OF DELAPRIL AND INDAPAMIDE IN TABLETS

2017 ◽  
Vol 1 (1) ◽  
pp. 50-55
Author(s):  
Vitor Todeschini ◽  
Regiane Leite ◽  
Maximiliano Sangoi ◽  
Paulo Oliveira ◽  
Thiago Barth
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Partial Least Squares Regression ◽  
Fixed Dose ◽  
Least Squares Regression ◽  
Acceptable Range ◽  
Validation Parameters ◽  
Simple Alternative ◽  
Predict Model

A multicomponent ultraviolet (UV) spectrophotometric method was developed and validated for simultaneous determination of delapril (DEL) and indapamide (IND) in tablets employing the partial least squares regression (PLSR) approach. The PLSR method was developed by a multilevel factorial design using 25 synthetic mixtures of drugs and a significant predict model (p < 0.05) were obtained at 225 nm for DEL (r2 = 0.9992) and 243 nm for IND (r2 = 0.9997). Validation parameters such as the specificity, linearity, precision, accuracy and robustness were evaluated in accordance with the ICH requirements, giving satisfactory results within the acceptable range. The proposed PLSR method was successfully applied for simultaneous determination of DEL and IND in fixed dose combinations and can be used as simple alternative to separation techniques.

NOVEL UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF SITAGLIPTIN PHOSPHATE AND SIMVASTATIN BY AREA UNDER CURVE METHOD

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (04) ◽  
pp. 46-52
Author(s):  
V. V. Chopde ◽  
◽  
P. M. Patil ◽  
S. D Rathod ◽  
P. D. Chaudhari
Keyword(s):  
Simultaneous Determination ◽  
Wavelength Range ◽  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Fixed Dose Combination ◽  
Fixed Dose ◽  
Economic Method ◽  
Curve Method ◽  
Dose Combination

A simple, versatile, accurate, precise and economic method for simultaneous determination of sitagliptin phosphate and simvastatin in fixed dose combination products was developed. The absorbance values at 267 nm and 239 nm for sitagliptin phosphate and simvastatin. The combination is also estimated by AUC method it involved measurement of area under curve in the wavelength range is 264-270 nm (λ1 -λ2 ) and 236-242 nm (λ3 -λ4 ) sitagliptin phosphate and simvastatin respectively. This method obeyed Beer’s law in the concentration range of 10-60 μg /mL for sitagliptin phosphate and 2-12 μg /mL for simvastatin. The results of analyses have been validated statistically for linearity, accuracy, precision, LOD and LOQ of the proposed method.

Application of MEKC and UPLC with Fluorescence Detection for Simultaneous Determination of Amlodipine Besylate and Bisoprolol Fumarate

2020 ◽  
Author(s):  
Sohila M Elonsy ◽  
Fawzy A El Yazbi ◽  
Rasha A Shaalan ◽  
Hytham M Ahmed ◽  
Tarek S Belal
Keyword(s):  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Fused Silica ◽  
Effective Length ◽  
Fixed Dose ◽  
Array Detector ◽  
Internal Diameter ◽  
Amlodipine Besylate ◽  
Validation Parameters

Abstract Objective Two chromatographic methods were described for simultaneous determination of the antihypertensive drugs amlodipine besylate (AML) and bisoprolol fumarate (BIS). Methods Method I applies micellar electrokinetic capillary chromatography using a deactivated fused silica capillary (25 cm effective length × 50 μm internal diameter). The background electrolyte consisted of 0.01 M borate buffer (pH 9.2) containing 0.025 M sodium dodecyl sulphate and methanol in the ratio of 80:20 (v/v). Valsartan (VAL) was used as an internal standard. Diode array detector was set at 238, 224, and 210 nm for measuring AML, BIS, and VAL, respectively. Method II involves using ultra-performance liquid chromatography with fluorescence detection. Zorbax SB-C8 column (2.1 × 100 mm, 1.8 μm particle size) was used with isocratic elution of the mobile phase composed of 0.1% trifluoroacetic acid, acetonitrile, and methanol in the ratio of 55:35:10 (v/v) at a flow rate of 0.6 mL/min. Fluorescence detection was done using excitation wavelengths 230 and 370 nm and emission wavelengths 305 and 450 nm for BIS and AML, respectively. Validation parameters were carefully studied including linearity, ranges, precision, accuracy, robustness, detection, and quantification limits. Results Method I showed good linearity over the range 10–100 μg/mL for both dugs. Method II’s linear ranges were 0.001–0.1 and 0.02–1 µg/mL for BIS and AML, respectively. Conclusion The proposed methods were successfully validated and applied for assay of the studied drugs in their fixed-dose combination tablets. Highlights To the best of our knowledge, this study suggests the first electro-chromatographic and LC with fluorescence detection methods for simultaneous determination of amlodipine and bisoprolol.

Simultaneous Determination of Rubber Additives by FT-IR Spectrophotometry with Multivariate Calibration

1995 ◽  
Vol 49 (6) ◽  
pp. 747-753 ◽  
Author(s):  
M. Blanco ◽  
J. Coello ◽  
H. Iturriaga ◽  
S. Maspoch ◽  
E. Bertran
Keyword(s):  
Fourier Transform ◽  
Simultaneous Determination ◽  
Partial Least Squares Regression ◽  
Multivariate Calibration ◽  
Least Squares Regression ◽  
First Derivative ◽  
Vulcanized Rubber ◽  
Ir Spectrophotometry ◽  
Ft Ir

A new method for the simultaneous determination of vulcanized rubber additives by Fourier transform infrared (FT-IR) spectrophotometry using partial least-squares regression (PLSR) for multivariate calibration was developed. The effect of various wavenumber ranges and the use of the absorbance and first-derivative spectral modes on performance were studied by applying the method to three different sample batches containing several additives in different proportions, all of which were resolved with satisfactory results.

scholarly journals Simultaneous Determination of Atorvastatin and Amlodipine in Industrial Tablets by Apparent Content Curve and HPLC Methods

2013 ◽  
Vol 59 (1) ◽  
pp. 44-48
Author(s):  
Silvia Imre ◽  
Klára Kacsó ◽  
Daniela-Lucia Muntean
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Reference Method ◽  
Hplc Method ◽  
Test Method ◽  
Fixed Dose ◽  
Mathematical Approach ◽  
Curve Method ◽  
The Difference

AbstractIntroduction: This study proposes the simultaneous determination of atorvastatin and amlodipine in industrial tablets by a quantitative spectrophotometric method, named the apparent content curve method, test method, and by an HPLC method with UV detection as reference method.Materials and methods: A synthetic mixture and two fixed medicinal combinations containing amlodipine and atorvastatin were investigated by the apparent content curve method, a simple and relatively inexpensive UV-VIS spectrophotometric method based on a mathematical approach derived from the Lambert-Beer law. The results were compared with those obtained by an HPLC method.Results: A good correlation of the results was obtained, the difference between the pair results was not significant (p >0.05).Conclusions: The proposed spectrophotometric method is an easier and cheaper alternative for the quantitative determination of amlodipine and atorvastatin in industrial fixed-dose combinations.

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