Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

Abstract Date palm is one of the most important economical crops in the world. Sex determination of date palm in early stage is a prerequisite for breeding and cultivation. The aim of this study is to validate RAPD and ISSR markers for sex identification of date palm genotypes grown under Sudan conditions. DNA was extracted from ten seedlings and five male and female plants using CTAB method. Eight primers, six RAPD and two ISSR primers were examined for their validation in sex determination of date palm genotypes. PCR amplification was performed using these primers. Four RAPD primers OPA02, OPJ-09, RD A02 and RD A21 were amplified male specific band with size of 1000, 1100, 1000 and 1400 pb respectively, while ISSR markers could not. The specific bands were observed clearly among all male genotypes and absent in female samples unknown samples irrespective of genotypes. Our results could be useful for sex determination of date palm sex in seedling stage and would promote date palm cultivation and production.

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A PCR assay for sex determination of yak (Bos grunniens) meat by amplification of the male-specific SRY gene

Food Control ◽

10.1016/j.foodcont.2009.10.016 ◽

2010 ◽

Vol 21 (5) ◽

pp. 726-731 ◽

Cited By ~ 6

Author(s):

W.L. Bai ◽

R.H. Yin ◽

S.J. Zhao ◽

C. Li ◽

Z.J. Ma ◽

...

Keyword(s):

Sex Determination ◽

Sry Gene ◽

Pcr Assay ◽

Bos Grunniens ◽

Male Specific

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Sex Determination During Inflorescence Bud Differentiation in Monoecious Pistacia chinensis Bunge

Forests ◽

10.3390/f10030202 ◽

2019 ◽

Vol 10 (3) ◽

pp. 202 ◽

Cited By ~ 1

Author(s):

Qian Bai ◽

Chenyi Zhu ◽

Xia Lei ◽

Tao Cao ◽

Shuchai Su ◽

...

Keyword(s):

Sex Determination ◽

Early Stage ◽

Sex Differentiation ◽

Banding Patterns ◽

Vegetative Period ◽

Pcr Products ◽

Polymerase Chain ◽

Sex Determination Mechanisms

Pistacia chinensis Bunge is widely acknowledged to be dioecious, but rare monoecious individuals have been found. However, the origin of monoecism and the sex differentiation of different sex types remain intriguing questions. Here, sex expressions were explored by identification of sex-associated DNA markers, determination of the sex stability after grafting, and histological characterization of inflorescence bud development using anatomical analysis. The results showed that (1) although polymorphisms among individuals existed, the banding patterns of Polymerase Chain Reaction (PCR) products for different sex types on the same monoecious tree were consistent; (2) the sex expressions of grafted trees were not consistent with those of scions, indicating that monoecism probably did not originate from a stable bud mutation; and (3) both males and females underwent a bisexual period, then the stamen primordia in female buds degenerated into the second round tepals, while the pistil primordia in male buds gradually disappeared. During the sex differentiation phase, female buds were spindle-shaped, while the male buds were full teardrop-shaped, and male buds were bigger than female buds. Taken together, no sex-associated DNA marker was found, sex expressions were unstable after grafting, and the alternative sex organs appeared in the early stage of sex differentiation, suggesting that sex determination occurred during floral development instead of the early vegetative period. These results indicated that the sex expressions may be affected by environmental factors, increasing the understanding of sex determination mechanisms in P. chinensis and other species.

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Rapid Plant Regeneration and Molecular Assessment of Genetic Stability Using ISSR and RAPD Markers in Commercial Banana Cv. Grand Naine (G-9)

JOURNAL OF ADVANCES IN BIOTECHNOLOGY ◽

10.24297/jbt.v4i3.4890 ◽

2014 ◽

Vol 4 (3) ◽

pp. 393-403

Author(s):

J. S. DUHAN ◽

S. Kajla ◽

D. Choudhary ◽

A. K. Poonia

Keyword(s):

Tissue Culture ◽

Genetic Stability ◽

Random Amplified Polymorphic Dna ◽

Issr Markers ◽

Ms Medium ◽

Inter Simple Sequence Repeats ◽

Rapd Primer ◽

Molecular Assessment ◽

Rapd And Issr ◽

Issr Primers

The investigation was carried out to assess the genetic stability in Â Â tissue culture raised plants of banana cv. G-9 using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers.Aims:Â Molecular assessment of genetic stability of tissue culture raised plants of banana cv. G-9 using molecular markers.Material and Results: Apical shoots were established on mediumÂ EM4Â (MS + BAP 4.0 mg L-1) with maximum of 3.8 buds/explant in 2.6 days. The maximum bud multiplicationÂ withÂ 16.5Â±0.06 shootsÂ was observed on mediumÂ Ma3Â (MS medium+ 5.0 mg L-1Â BAP + 0.25 mg L-1Â NAA ofÂ + 30 mg L-1Â AdSO4).Â The maximum rooting response (100%) wasÂ observed on 1/2 MS medium supplemented with 2.0 mg L-1Â NAA inÂ 12.2 days. After acclimatization the hardened plants were examined for genetic stability using RAPD and ISSR primers. Total forty six (twenty six RAPD and twenty ISSR) markers were used. RAPD primers produced 87 distinct and scorable bands, with an average of 3.34 bands per primer and the amplification products range was from 100-1200 bps. The number of scorable bands for RAPD primer varied from 2 to 5 with an average of 3.34 bands per primer. ISSR primers produced 71 distinct and scorable bands in the range of 100-1000 bps and the number of scorable bands for each primer varied from 2 to 6 with an average of 3.55 bands per primer.Conclusion: Similar profile with monomorphic bands was observed for all the tissue culture raised plants when compared to mother plant in both types of markers used. The results corroborate the fact that plant tissue culture technology has immense importance for production of true to type of planting material.Â

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Cytogenetic and molecular studies on two faba bean cultivars revealed their difference in their aluminum tolerance

Acta agriculturae Slovenica ◽

10.14720/aas.2020.116.2.1346 ◽

2020 ◽

Vol 116 (2) ◽

pp. 273

Author(s):

Ahmed M. HASSANEIN ◽

Ahmed H. MOHAMED ◽

Heba Ahmed ABD ALLAH ◽

Hoida ZAKI

Keyword(s):

Faba Bean ◽

Chromosomal Abnormalities ◽

Aluminum Tolerance ◽

Issr Markers ◽

Growth Data ◽

Al Tolerance ◽

Al Stress ◽

Molecular Studies ◽

Rapd And Issr ◽

Issr Primers

Two cultivars of faba bean (Vicia faba ‘Giza 843’ and ‘Nobaria 3’) that differ in aluminum (Al) tolerance were used to study cytogenetic and genomic alterations under the influence of Al Cl3 (5, 15, and 25 mmol AlCl3) for different periods (6, 12 and 24 h). Under Al treatments, mitotic index in both cultivars decreased and total chromosomal abnormalities increased. The frequencies of micronuclei and chromosomal abnormalities (C-anaphase, metaphase-star chromosomes, breaks, sticky and disturbed chromosomes during metaphase or anaphase) in ‘Giza 843’ were lower than in ‘Nabaria 3’. Increase of the registered cytogenetic events under the influence of Al stress led to increase the detected polymorphism using RAPD and ISSR markers. Application of RAPD primers gave the same value of polymorphism in both faba bean cultivars under Al stress. Polymorphism average of nine ISSR primers of ’Giza 843’ (65.36 %) was lower than that of ‘Nobaria 3’ (71.59 %). Molecular markers, cytogenetic characteristics and seedling growth data indicate that Al tolerance of ‘Giza 843’ was higher than of ‘Nobaria 3’. This work shows that cytogenetic and ISSR techniques could be used efficiently to distinguish between the ability of two faba bean cultivars to tolerate toxic effects of Al.

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Assessment of seed quality and sex determination in hop seedlings using molecular markers

Ciência e Natura ◽

10.5902/2179460x43135 ◽

2020 ◽

Vol 42 ◽

pp. e45

Author(s):

Marília Pereira Machado ◽

Andreza Cerioni Belniaki ◽

André Felipe Bernert ◽

Erik Nunes Gomes ◽

João Carlos Bespalhok Filho ◽

...

Keyword(s):

Molecular Markers ◽

Sex Determination ◽

Seed Quality ◽

Germination Percentage ◽

Brewing Industry ◽

Breeding Programs ◽

Normal Seedling ◽

Physiological Quality ◽

Male Specific

Brazil is the world's third largest beer consumer and currently imports all of its hops for the brewing industry. Such a fact justifies the selection of hop genotypes adapted for cultivation locally, which requires high quality seeds and efficient sex determination of the seedlings. The objectives of this study were to develop a methodology to assess hop seed quality and to efficiently determine hop seedling sex through the use of male-specific molecular markers. Freshly harvested hop seeds were germinated with and without pre-chilling (3-5 ° C) for 3, 6 and 12 weeks and then germinated at 20 or 25 ° C in the presence or absence of light, evaluating germination percentage and germination speed index. F1 progenies were obtained from after seed germination in a greenhouse and seedlings sex was determined using male-specific molecular markers. The best conditions for physiological quality assessment of hop seeds used in the present study were pre-chilling for 12 weeks, followed by germination at 25 ° C, and normal seedling counts at 7 and 15 days. The progeny submitted to molecular marker sexing was composed of 61.3% female plants. The established methodologies presented here can be considered efficient and may contribute to expedite hops breeding programs.

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Sex Determination of Equine Somatic and Germ Cells by PCR Amplification Based on the Sequence Polymorphism of X- and Y-Chromosomal Amelogenin Genes

Nihon Chikusan Gakkaiho ◽

10.2508/chikusan.70.7_6 ◽

1999 ◽

Vol 70 (7) ◽

pp. 6-10 ◽

Cited By ~ 1

Author(s):

Yasutoshi FUKUSHIMA ◽

Harutaka MUKOYAMA ◽

Fumio SATO ◽

Telhisa HASEGAWA ◽

Nobushige ISHIDA ◽

...

Keyword(s):

Sex Determination ◽

Germ Cells ◽

Pcr Amplification ◽

Sequence Polymorphism

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Identification and sequencing of Date-SRY Gene: A novel tool for sex determination of date palm (Phoenix dactylifera L.)

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2017.08.002 ◽

2019 ◽

Vol 26 (3) ◽

pp. 514-523 ◽

Cited By ~ 4

Author(s):

Mohei EL-Din Solliman ◽

Heba Allah A. Mohasseb ◽

Abdullatif A. Al-Khateeb ◽

Suliman A. Al-Khateeb ◽

Kamal Chowdhury ◽

...

Keyword(s):

Sex Determination ◽

Date Palm ◽

Phoenix Dactylifera ◽

Sry Gene ◽

Phoenix Dactylifera L

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Identification and Validation of a New Male Sex-Specific ISSR Marker in Pointed Gourd (Trichosanthes dioicaRoxb.)

The Scientific World JOURNAL ◽

10.1155/2014/216896 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 3

Author(s):

Sinchan Adhikari ◽

Soumen Saha ◽

Tapas Kumar Bandyopadhyay ◽

Parthadeb Ghosh

Keyword(s):

Pcr Amplification ◽

Specific Marker ◽

Reliable Technique ◽

Sequence Characterization ◽

Gender Determination ◽

Pooled Dna ◽

Sex Marker ◽

Pointed Gourd ◽

Male Specific ◽

Issr Primers

The aim of the present study was to develop a genetic sex marker for the pointed gourd (Trichosanthes dioicaRoxb.) to allow gender determination at any stage in the life cycle. Screening of genomic DNA with intersimple sequence repeat (ISSR) primers was used to discover sex-specific touch-down polymerase chain reaction (Td-PCR) amplification products. Using pooled DNA from male and female genotypes and 42 ISSR primers, a putative male specific marker (~550 bp) was identified. DNA marker specific to male is an indication of existence of nonepigenetic factors involved in gender development in pointed gourd. The ISSR technique has proved to be a reliable technique in gender determination of pointed gourd genotypes at the seedling phenophase. The sex marker developed here could also be used as a starting material towards sequence characterization of sex linked genes for better understanding the developmental as well as evolutionary pathways in sexual dimorphism.

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