scholarly journals Rapid Plant Regeneration and Molecular Assessment of Genetic Stability Using ISSR and RAPD Markers in Commercial Banana Cv. Grand Naine (G-9)

2014 ◽  
Vol 4 (3) ◽  
pp. 393-403
Author(s):  
J. S. DUHAN ◽  
S. Kajla ◽  
D. Choudhary ◽  
A. K. Poonia
Keyword(s):  
Tissue Culture ◽  
Genetic Stability ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Ms Medium ◽  
Inter Simple Sequence Repeats ◽  
Rapd Primer ◽  
Molecular Assessment ◽  
Rapd And Issr ◽  
Issr Primers

The investigation was carried out to assess the genetic stability in   tissue culture raised plants of banana cv. G-9 using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers.Aims:  Molecular assessment of genetic stability of tissue culture raised plants of banana cv. G-9 using molecular markers.Material and Results: Apical shoots were established on medium EM4 (MS + BAP 4.0 mg L-1) with maximum of 3.8 buds/explant in 2.6 days. The maximum bud multiplication with 16.5±0.06 shoots was observed on medium Ma3 (MS medium+ 5.0 mg L-1 BAP + 0.25 mg L-1 NAA of + 30 mg L-1 AdSO4). The maximum rooting response (100%) was observed on 1/2 MS medium supplemented with 2.0 mg L-1 NAA in 12.2 days. After acclimatization the hardened plants were examined for genetic stability using RAPD and ISSR primers. Total forty six (twenty six RAPD and twenty ISSR) markers were used. RAPD primers produced 87 distinct and scorable bands, with an average of 3.34 bands per primer and the amplification products range was from 100-1200 bps. The number of scorable bands for RAPD primer varied from 2 to 5 with an average of 3.34 bands per primer. ISSR primers produced 71 distinct and scorable bands in the range of 100-1000 bps and the number of scorable bands for each primer varied from 2 to 6 with an average of 3.55 bands per primer.Conclusion: Similar profile with monomorphic bands was observed for all the tissue culture raised plants when compared to mother plant in both types of markers used. The results corroborate the fact that plant tissue culture technology has immense importance for production of true to type of planting material. 

Assessment of Genetic Stability and Instability of Tissue Culture-Propagated Plantlets of Aloe vera L. by RAPD and ISSR Markers

2011 ◽  
Vol 165 (5-6) ◽  
pp. 1356-1365 ◽  
Author(s):  
Mangal Singh Rathore ◽  
J. Chikara ◽  
Shaik G. Mastan ◽  
H. Rahman ◽  
K. G. V. Anand ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Genetic Stability ◽  
Aloe Vera ◽  
Issr Markers ◽  
Stability And Instability ◽  
Rapd And Issr

Comparative assessment of genetic diversity among twenty varieties of Brassica juncea [L.] Czern & Coss using RAPD and ISSR markers

2012 ◽  
Vol 2 (4) ◽  
pp. 166-176
Author(s):  
Javed Ahmad ◽  
Salim Khan ◽  
Gohar Taj Khan
Keyword(s):  
Genetic Diversity ◽  
Brassica Juncea ◽  
Genetic Similarity ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Rapd Profile ◽  
Inter Simple Sequence Repeats ◽  
Rapd And Issr ◽  
Simple Sequence

PCR based molecular markers such as RAPD (random amplified polymorphic DNA) and ISSR (inter simple sequence repeats) were employed for the evaluation of genetic diversity among twenty varieties of Brassica juncea. Mean polymorphism information content (PIC) value was greater for RAPD (0.4195) as compared to ISSR (0.2602). In RAPD analysis, 98.9% loci were polymorphic whereas in ISSR, 94.8 % were polymorphic. The number of loci in RAPD profile ranged from 7 to 10 with an average of 9.3 per primer whereas in ISSR, these were from 3 to 12 with an average of 6.8 loci per primer. RAPD based genetic similarity ranged from 0.224 to 0.842 whereas ISSR derived genetic similarity 0.467 to 0.880. The mental test between two Jaccard’s similarity matrices gave r = 0.89, showing good fit correlation in between ISSR‐ and RAPD‐based similarities. The results obtained from the consensus tree constructed from RAPD+ISSR marker more likely support the distribution of the twenty genotypes of B. juncea based on ISSR analysis. The twenty varieties were clustered into three main clusters 1, 2, and 3 respectively. In combined dendrogram study, each cluster has 13, 3, and 4 varieties.

scholarly journals Genetic Fidelity in Micropropagated Plantlets of Pimpinella tirupatiensis-an Endemic and Threatened Medicinal Plant Using RAPD and ISSR Markers

2016 ◽  
Vol 5 (06) ◽  
pp. 4661
Author(s):  
Srilakshmi A.* ◽  
Ugraiah A. ◽  
Gayatri C. M. ◽  
Rajanna L.
Keyword(s):  
Acetic Acid ◽  
Large Scale ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Fidelity ◽  
Issr Markers ◽  
Scale Production ◽  
Ms Medium ◽  
Threatened Medicinal Plant ◽  
Issr Primers

Tissue cultured Pimpinella tirupatiensis plantlets were subjected to assessment of genetic stability considering the fact that associated in vitro stress might result in breakdown of control mechanism causing instability of the genome. We have used two DNA based molecular markers to assess the genetic fidelity of in vitro regenerated Pimpinella tirupatiensis through shoot tip from in vitro raised seedlings. The shoot tips upon transfer to MS medium containing different concentrations and combinations of 6- benzyl aminopurine (BAP) kinetin (KN), 2- isopentyl adenine (2- ip), α- napthalene acetic acid (NAA) and indole 3- acetic acid (IAA). The best morphogenic response was observed on MS medium fortified with BA (13.31 µM) and NAA (2.69 µM) which exhibited the highest regeneration frequency (90%), the maximum number of shoots/explants (6.50 ± 0.91) and shoot length (3.20 ± 0.20) within 5 weeks. Rooting was achieved within 15 days of shoot implantation on ½ strength MS media fortified with BAP (13.31 µM) and IBA (9.8 µM). The rooted plantlets were successfully acclimatized with 85% survival rate. Out of 20 RAPD and 3 ISSR primers screened, only 6 random amplified polymorphic DNA (RAPD) and all three inter simple sequence repeats (ISSR) primers produced clear reproducible and scorable bands. All banding profiles from micropropagated plants were monomorphic and similar to the mother plant indicating an absence of noticeable genetic variation in the regenerated plantlets. This study is of high significance as these could be commercially utilized for large scale production of true-to-type plantlets in Pimpinella tirupatiensis.

Species-diagnostic and species-specific DNA sequences evenly distributed throughout pine and spruce chromosomes

Genome ◽  
2010 ◽  
Vol 53 (10) ◽  
pp. 769-777 ◽  
Author(s):  
Melanie Mehes-Smith ◽  
Paul Michael ◽  
Kabwe Nkongolo
Keyword(s):  
Dna Sequences ◽  
Random Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Pinus Monticola ◽  
The Family ◽  
Species Specific ◽  
Rapd And Issr ◽  
Specific Sequences

Genome organization in the family Pinaceae is complex and largely unknown. The main purpose of the present study was to develop and physically map species-diagnostic and species-specific molecular markers in pine and spruce. Five RAPD (random amplified polymorphic DNA) and one ISSR (inter-simple sequence repeat) species-diagnostic or species-specific markers for Picea mariana , Picea rubens , Pinus strobus , or Pinus monticola were identified, cloned, and sequenced. In situ hybridization of these sequences to spruce and pine chromosomes showed the sequences to be present in high copy number and evenly distributed throughout the genome. The analysis of centromeric and telomeric regions revealed the absence of significant clustering of species-diagnostic and species-specific sequences in all the chromosomes of the four species studied. Both RAPD and ISSR markers showed similar patterns.

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

Micropropagation of Zingiber rubens and assessment of genetic stability through RAPD and ISSR markers

2011 ◽  
Vol 55 (1) ◽  
pp. 16-20 ◽  
Author(s):  
S. Mohanty ◽  
M. K. Panda ◽  
S. Sahoo ◽  
S. Nayak
Keyword(s):  
Genetic Stability ◽  
Issr Markers ◽  
Rapd And Issr

Clonal structure and reduced diversity of the invasive alien plant Erigeron annuus in Lithuania

2013 ◽  
Vol 8 (9) ◽  
pp. 898-911 ◽  
Author(s):  
Jolanta Patamsytė ◽  
Vytautas Rančelis ◽  
Tatjana Čėsnienė ◽  
Violeta Kleizaitė ◽  
Virginija Tunaitienė ◽  
...  
Keyword(s):  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Genetic Distances ◽  
Natural Ecosystems ◽  
Alien Plant ◽  
Invasive Alien Plant ◽  
Inter Simple Sequence Repeats ◽  
Upgma Cluster Analysis ◽  
Erigeron Annuus

AbstractThe alien species Erigeron annuus (L.) Pers. is in an intensive spreading phase in Lithuania. Random amplified polymorphic DNA (RAPDs) and inter-simple sequence repeats (ISSRs) assays were used to study the genetic structure of old and new invasive populations and to determine the most spread genotypes of this species in Lithuania. Pairwise genetic distances between populations established using RAPD and ISSR markers significantly correlated (r=0.91, P<0.05). Our study indicates that there are two genetically different types of E. annuus populations. The first type is represented by a widely spread main clone and related monomorphic populations. The second type is represented by polymorphic populations, some of them present at sites where E. annuus has not been previously observed. Main clone predominates in nine populations and is from the region where this species was first described in natural ecosystems of Lithuania. UPGMA cluster analysis revealed genetic relationships between the main clone and accessions from old cemeteries where E. annuus has been grown as an ornamental plant. We found high genetic differentiation among populations (G ST=0.58 for RAPDs, G ST=0.64 for ISSRs). Taken together, our results will contribute to the monitoring of E. annuus spread in Lithuania.

Genetic diversity and relationships among Egyptian Galium (Rubiaceae) and related species using ISSR and RAPD markers

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Kadry Abdel Khalik ◽  
Magdy Abd El-Twab ◽  
Rasha Galal
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Phylogenetic Analyses ◽  
Randomly Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeats ◽  
Close Relationship ◽  
Rapd And Issr ◽  
Simple Sequence ◽  
Issr Primers

AbstractGenetic diversity and phylogenetic analyses of 24 species, representing nine sections of the genus Galium (Rubiaceae), have been made using the Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and combined ISSR and RAPD markers. Four ISSR primers and three RAPD primers generated 250 polymorphic amplified fragments. The results of this study showed that the level of genetic variation in Galium is relatively high. RAPD markers revealed a higher level of polymorphism (158 bands) than ISSR (92 bands). Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrograms were compared. Six clades can be recognized within Galium, which mostly corroborate, but also partly contradict, traditional groupings. UPGMA-based dendrogram showed a close relationship between members of section Leiogalium with G. verum and G. humifusum (sect. Galium), and G. angustifolium (sect. Lophogalium). Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. The more apomorphic groups of Galium form the section Leiogalium clade including the perennial sections Galium, Lophogalium, Jubogalium, Hylaea and Leptogalium as well as the annual section Kolgyda. The remaining taxa of Galium are monophyletic.

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