scholarly journals Cytogenetic and molecular studies on two faba bean cultivars revealed their difference in their aluminum tolerance

2020 ◽  
Vol 116 (2) ◽  
pp. 273
Author(s):  
Ahmed M. HASSANEIN ◽  
Ahmed H. MOHAMED ◽  
Heba Ahmed ABD ALLAH ◽  
Hoida ZAKI
Keyword(s):  
Faba Bean ◽  
Chromosomal Abnormalities ◽  
Aluminum Tolerance ◽  
Issr Markers ◽  
Growth Data ◽  
Al Tolerance ◽  
Al Stress ◽  
Molecular Studies ◽  
Rapd And Issr ◽  
Issr Primers

<p>Two cultivars of faba bean (<em>Vicia faba</em> ‘Giza 843’ and ‘Nobaria 3’) that differ in aluminum (Al) tolerance were used to study cytogenetic and genomic alterations under the influence of Al Cl<sub>3</sub> (5, 15, and 25 mmol AlCl<sub>3</sub>) for different periods (6, 12 and 24 h). Under Al treatments, mitotic index in both cultivars decreased and total chromosomal abnormalities increased. The frequencies of micronuclei and chromosomal abnormalities (C-anaphase, metaphase-star chromosomes, breaks, sticky and disturbed chromosomes during metaphase or anaphase) in ‘Giza 843’ were lower than in ‘Nabaria 3’. Increase of the registered cytogenetic events under the influence of Al stress led to increase the detected polymorphism using RAPD and ISSR markers. Application of RAPD primers gave the same value of polymorphism in both faba bean cultivars under Al stress. Polymorphism average of nine ISSR primers of ’Giza 843’ (65.36 %) was lower than that of ‘Nobaria 3’ (71.59 %). Molecular markers, cytogenetic characteristics and seedling growth data indicate that Al tolerance of ‘Giza 843’ was higher than of ‘Nobaria 3’. This work shows that cytogenetic and ISSR techniques could be used efficiently to distinguish between the ability of two faba bean cultivars to tolerate toxic effects of Al.</p>

The zinc finger transcription factor ATF1 regulates aluminum tolerance in barley

2020 ◽  
Vol 71 (20) ◽  
pp. 6512-6523
Author(s):  
Liyuan Wu ◽  
Yiyi Guo ◽  
Shengguan Cai ◽  
Liuhui Kuang ◽  
Qiufang Shen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Activation ◽  
Crop Production ◽  
Molecular Mechanisms ◽  
Aluminum Tolerance ◽  
Acid Soils ◽  
Al Tolerance ◽  
Al Stress ◽  
Transcription Factor 1 ◽  
Differently Expressed Genes

Abstract Aluminum (Al) toxicity is a major abiotic stress that restricts crop production in acid soils. Plants have evolved internal and external mechanisms of tolerance, and among them it is well known that AtSTOP1 and OsART1 are key transcription factors involved in tolerance through regulation of multiple downstream genes. Here, we identified the closest homolog of these two proteins in barley, namely HvATF1, Al-tolerance Transcription Factor 1, and determined its potential function in Al stress. HvATF1 is expressed in the nucleus, and functions in transcriptional activation. The transcription of HvATF1 was found to be constitutive in different tissues, and was little affected by Al stress. Knockdown of HvATF1 by RNAi resulted in increased Al sensitivity. Transcriptomics analysis identified 64 differently expressed genes in the RNAi lines compared to the wild-type, and these were considered as candidate downstream genes regulated by HvATF1. This study provides insights into the different molecular mechanisms of Al tolerance in barley and other plants.

scholarly journals Rapid Plant Regeneration and Molecular Assessment of Genetic Stability Using ISSR and RAPD Markers in Commercial Banana Cv. Grand Naine (G-9)

2014 ◽  
Vol 4 (3) ◽  
pp. 393-403
Author(s):  
J. S. DUHAN ◽  
S. Kajla ◽  
D. Choudhary ◽  
A. K. Poonia
Keyword(s):  
Tissue Culture ◽  
Genetic Stability ◽  
Random Amplified Polymorphic Dna ◽  
Issr Markers ◽  
Ms Medium ◽  
Inter Simple Sequence Repeats ◽  
Rapd Primer ◽  
Molecular Assessment ◽  
Rapd And Issr ◽  
Issr Primers

The investigation was carried out to assess the genetic stability in   tissue culture raised plants of banana cv. G-9 using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers.Aims:  Molecular assessment of genetic stability of tissue culture raised plants of banana cv. G-9 using molecular markers.Material and Results: Apical shoots were established on medium EM4 (MS + BAP 4.0 mg L-1) with maximum of 3.8 buds/explant in 2.6 days. The maximum bud multiplication with 16.5±0.06 shoots was observed on medium Ma3 (MS medium+ 5.0 mg L-1 BAP + 0.25 mg L-1 NAA of + 30 mg L-1 AdSO4). The maximum rooting response (100%) was observed on 1/2 MS medium supplemented with 2.0 mg L-1 NAA in 12.2 days. After acclimatization the hardened plants were examined for genetic stability using RAPD and ISSR primers. Total forty six (twenty six RAPD and twenty ISSR) markers were used. RAPD primers produced 87 distinct and scorable bands, with an average of 3.34 bands per primer and the amplification products range was from 100-1200 bps. The number of scorable bands for RAPD primer varied from 2 to 5 with an average of 3.34 bands per primer. ISSR primers produced 71 distinct and scorable bands in the range of 100-1000 bps and the number of scorable bands for each primer varied from 2 to 6 with an average of 3.55 bands per primer.Conclusion: Similar profile with monomorphic bands was observed for all the tissue culture raised plants when compared to mother plant in both types of markers used. The results corroborate the fact that plant tissue culture technology has immense importance for production of true to type of planting material. 

scholarly journals (.ISSR Markers Associated With Effects of Gamma Irradiation on Growth and Seed Yield of M2 Plants of Faba Bean (Vicia faba L

2016 ◽  
Vol 2 (2) ◽  
Author(s):  
Nora El-Gazzar , Laila Mekki , Samia Heneidak, Abdelfattah B
Keyword(s):  
Faba Bean ◽  
Growth Parameters ◽  
Polymorphic Information Content ◽  
Issr Markers ◽  
Growth And Yield ◽  
Yield Parameters ◽  
Polymorphic Bands ◽  
And Control ◽  
Higher Doses ◽  
Issr Primers

Dry seeds of six faba bean varieties (Sakha 1, Nubaria 2, Nubaria 3, Giza 3, Giza 716 and Giza  843) were irradiated with low doses of gamma rays (20, 40, 60, 80, 100 and 200 Gy). Treated seeds and control seeds were grown for two seasons. Results revealed that doses below 100 Gy increased morphological  growth parameters as well as some yield parameters. The most effective dose varied in different faba bean varieties. Higher doses above 100 Gy were detrimental to plant growth and yield. The variation in ISSR profiling in response to g-irradiation treatments in M2 was detected using seven ISSR primers. The seven primers produced 81 bands including 75 polymorphic bands and 6 unique bands. The unique bands were scored in var. Nubaria 3 by the doses (20, 80 and 100 Gy), var. Giza 3 by the doses (20 and 60 Gy) and var. Nubaria 2 by the dose 200 Gy. The two varieties Nubaria 3 and Nubaria 2 showed higher numbers of polymorphic bands (225, 191) respectively compared to other varieties. Polymorphic information content (PIC) was estimated for each primer and ranged from 0.193 for primer 807 to 0.31 for primer 834 with a mean of 0.259.

Genetic Relationship in Sainfoin (Onobrychis viciifolia) Landraces Cultivated East Anatolia by using RAPD and ISSR Markers

2021 ◽  
Author(s):  
Mehmet Macit Ertuş
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
Calcareous Soils ◽  
Similarity Index ◽  
Issr Markers ◽  
High Genetic Diversity ◽  
Onobrychis Viciifolia ◽  
Young Leaves ◽  
Rapd And Issr ◽  
Issr Primers

Background: Sainfoin (Onobrychis viciifolia) is a forage crop that yields high in arid and calcareous soils and is cultivated in large areas. There aren’t many genetic diversity studies on the varieties of cultured sainfoin. This study was conducted to determine the genetic diversity and the degree of relationship between 23 cultivated landraces and one registered variety. Methods: To take samples from the populations, seeds were sown in the field in 2014. Samples were taken from the young leaves of the plants and preserved at -80oC in same year. RAPD and ISSR primers were used in the study. The bands obtained as a result of PCR were recorded and the data of both methods were also evaluated by combining them. Result: In the study, 5 RAPD and 4 ISSR primers were used and a total of 49 bands were obtained. Of 29 bands obtained using RAPD primers, 20 were found to be polymorphic and of 20 bands obtained using ISSR primers, 15 were found to be polymorphic. It was found that there was a very low correlation between the two methods. Using RAPD and ISSR markers and RAPD + ISSR combination, the similarity index among populations was found to be between 0.25-0.95, 0.5-1.00 and 0.45-0.91, respectively. The Nei’s genetic diversity index was found to be between 0.3365, 0.2656 and 0.3018 with RAPD, ISSR primers and RAPD + ISSR combination, respectively. Based on the dendrograms obtained using RAPD, ISSR primers and RAPD + ISSR combination, the populations under analysis were classified into 3, 3 and 5 groups, respectively. With this study, the closest populations were identified and a significantly high genetic diversity was detected.

scholarly journals Molecular Characterization and Analysis of the Greek Citrus Germplasm

2013 ◽  
Vol 41 (2) ◽  
pp. 463 ◽  
Author(s):  
Constantinos TRIPOLITSIOTIS ◽  
Nikolaos NIKOLOUDAKIS ◽  
Athanasios LINOS ◽  
Marianna HAGIDIMITRIOU
Keyword(s):  
Sweet Orange ◽  
Issr Markers ◽  
Molecular Techniques ◽  
Discrimination Power ◽  
Breeding Programs ◽  
High Affinity ◽  
Citrus Germplasm ◽  
Highly Correlated ◽  
Rapd And Issr ◽  
Issr Primers

Molecular markers were used to evaluate genetic similarity among 36 citrus accessionsc (C. sinensis, C. reticulata, C. limon and cultivars) of the Greek national clonal germplasm repository located in Poros. In total, nine RAPD and five ISSR primers produced 262 loci, of which 236 were polymorphic. Both techniques were proven to be equally analytical with an average discrimination power above 0.9. The RAPD and ISSR markers were highly correlated and clustering among them had high correspondence. Citrus accessions formed separate clusters according to their species, even though sweet orange and mandarin cultivars revealed high affinity, while lemons were more divergent. C. sinensis genotypes had the highest intragenetic affinity suggesting that cultivated oranges have a narrow genetic base. On the contrary, the C. reticulata group was the most variable. In general, Greek varieties exhibited a distinctive genetic background that could be discriminated from foreign established cultivars using the mentioned molecular techniques. This information could facilitate their use in future Citrus spp. breeding programs.

scholarly journals RAPD and ISSR Methods Used for Fingerprinting of Selected Accessions of Viburnum

2014 ◽  
Vol 6 (3) ◽  
pp. 292-299
Author(s):  
Marcelina KRUPA-MAŁKIEWICZ ◽  
Miłosz Smolik ◽  
Anna BARNIAK ◽  
Beata SMOLIK
Keyword(s):  
Genetic Variability ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Mantel Test ◽  
North West ◽  
The North ◽  
West Part ◽  
Rapd And Issr ◽  
Simple Sequence ◽  
Issr Primers

Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were used to investigate genetic variability within thirteen Viburnum species (Viburnum × hillieri; V. dilatatum; Viburnum × carlcephalum; V. opulus; V. hupehense; Viburnum× bodnantense; Viburnum × burkwoodii; V. sieboldii; Viburnum × globosum ‘Jermyns Globe’; V. alnifolium (lantanoides); V. plicatum ‘Sterile’; V. plicatum f. tomentosum and V. plicatum ‘Watanabe’) of wide geographical distribution, collected in the Dendrological Garden in Przelewice (the north-west part of Poland). Twenty-three RAPD and fourteen ISSR primers generated a total of 690 and 418 reproducible bands, respectively, and 39% (RAPD) and 55.5% (ISSR) of them were polymorphic for the two marker systems, which suggest high genetic variability within Viburnum genus. However, high numbers of genotype-specific bands, i.e. 60.9% (RAPD) and 44.5% (ISSR), were seen in Viburnum. Genetic similarity assessed within Viburnum species with the RAPD and ISSR analyses ranged from 6 to 42% and from 6 to 31%, respectively. Both RAPD and ISSR-based dendrograms clustered in five main groups. The Mantel test between two Nei’s similarity matrices gave correlation coefficient r=0.305*, showing low correlation between RAPD- and ISSR- based matrices. Thus, both marker systems were equally important for the genetic diversity analysis in Viburnum genus.

scholarly journals Overexpression of the Aldehyde Dehydrogenase Gene ZmALDH Confers Aluminum Tolerance in Arabidopsis thaliana

2022 ◽  
Vol 23 (1) ◽  
pp. 477
Author(s):  
Han-Mei Du ◽  
Chan Liu ◽  
Xin-Wu Jin ◽  
Cheng-Feng Du ◽  
Yan Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aldehyde Dehydrogenase ◽  
Aluminum Tolerance ◽  
Al Toxicity ◽  
Al Tolerance ◽  
Al Stress ◽  
Dehydrogenase Gene ◽  
Glutathione Cycle ◽  
Excessive Accumulation ◽  
Main Factor

Aluminum (Al) toxicity is the main factor limiting plant growth and the yield of cereal crops in acidic soils. Al-induced oxidative stress could lead to the excessive accumulation of reactive oxygen species (ROS) and aldehydes in plants. Aldehyde dehydrogenase (ALDH) genes, which play an important role in detoxification of aldehydes when exposed to abiotic stress, have been identified in most species. However, little is known about the function of this gene family in the response to Al stress. Here, we identified an ALDH gene in maize, ZmALDH, involved in protection against Al-induced oxidative stress. Al stress up-regulated ZmALDH expression in both the roots and leaves. The expression of ZmALDH only responded to Al toxicity but not to other stresses including low pH and other metals. The heterologous overexpression of ZmALDH in Arabidopsis increased Al tolerance by promoting the ascorbate-glutathione cycle, increasing the transcript levels of antioxidant enzyme genes as well as the activities of their products, reducing MDA, and increasing free proline synthesis. The overexpression of ZmALDH also reduced Al accumulation in roots. Taken together, these findings suggest that ZmALDH participates in Al-induced oxidative stress and Al accumulation in roots, conferring Al tolerance in transgenic Arabidopsis.

scholarly journals Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers

Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 415-423
Author(s):  
Saida Hasanova ◽  
Zeynal Akparov ◽  
Alamdar Mammadov ◽  
Litfer Amirov ◽  
Sevda Babayeva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Diversity Index ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Parental Lines ◽  
Spss Software ◽  
Rapd And Issr ◽  
Combined Data ◽  
Issr Primers

Genetic diversity of 62 chickpea accessions was studied using 8 ISSR and 11 RAPD primers. In the study RAPD primers detected more polymorphism (98%) than the ISSR primers (80%). Genetic diversity index was high (0.73 for ISSR and 0.85 for RAPD) for each of these marker systems. Cluster analysis performed from both separate and combined data of RAPD and ISSR markers using SPSS software package. Jaccard?s similarity coefficient for 62 chickpea genotypes was 0.65. Cluster analyses based on combined data generated a dendrogram that separated genotypes into 11 clusters. Four clusters contained only one genotype showing the genetic uniqueness of these accessions. The studied chickpea collection has been proved to constitute a rich source of biodiversity as revealed by RAPD and ISSR markers. Crossing between distantly related genotypes is expected to yield more vigorous plants constituting much of the different traits contained in the two parental lines.

scholarly journals Validation of RAPD and ISSR Markers Used for Sex Determination in Date Palm Grown under Sudan Conditions

2019 ◽  
Vol 3 (1) ◽  
pp. 17-22
Author(s):  
Ismail A. Mohammed ◽  
Israa A. Mohamed
Keyword(s):  
Sex Determination ◽  
Date Palm ◽  
Early Stage ◽  
Pcr Amplification ◽  
Issr Markers ◽  
Ctab Method ◽  
Male Specific ◽  
Rapd And Issr ◽  
Issr Primers

Abstract Date palm is one of the most important economical crops in the world. Sex determination of date palm in early stage is a prerequisite for breeding and cultivation. The aim of this study is to validate RAPD and ISSR markers for sex identification of date palm genotypes grown under Sudan conditions. DNA was extracted from ten seedlings and five male and female plants using CTAB method. Eight primers, six RAPD and two ISSR primers were examined for their validation in sex determination of date palm genotypes. PCR amplification was performed using these primers. Four RAPD primers OPA02, OPJ-09, RD A02 and RD A21 were amplified male specific band with size of 1000, 1100, 1000 and 1400 pb respectively, while ISSR markers could not. The specific bands were observed clearly among all male genotypes and absent in female samples unknown samples irrespective of genotypes. Our results could be useful for sex determination of date palm sex in seedling stage and would promote date palm cultivation and production.

scholarly journals Morphological And Molecular Identification of Double Flowered Stock (Matthiola Incana R. Br) Cultivars With High Fertility

2021 ◽  
Author(s):  
Un-Hyang Ho ◽  
Jong-Hyang Ri ◽  
Chol-Jun Ri
Keyword(s):  
Morphological Characteristics ◽  
Issr Markers ◽  
High Fertility ◽  
Leaf Color ◽  
Double Flower ◽  
Matthiola Incana ◽  
Commercially Important ◽  
Rapd And Issr ◽  
Commercial Stock ◽  
Issr Primers

Abstract Garden stock (Matthiola incana R. Br.) is a commercially important horticultural crop owing to its ornamental effect. There are different stock cultivars varied in color and shape, especially flowered phenotype is an essential index evaluating its commercial value, because double flowered cultivars have more brilliant flowers compared to single flowered one. The present work aimed: (1) to make superior cultivars with different colors, high fertility, being capable of early selecting only double flowered seedlings by leaf color and to investigate morphological characteristics and (2) to select RAPD and ISSR primers for the cultivar certification and identification to culture and produce good commercial stock cultivars. Here we obtained new double flowered stock cultivars with different colors including pink, pale pink and white, through outcrossing between “white” cultivar (high fertile but unable to select double flower phenotype) and “pink” cultivar (vice. versa). Among newly obtained stock cultivars, single and double flower seedlings are distinguishable from each other by leaf color, having about 70% of fertility. Moreover RAPD and ISSR markers selected in this study can be applied to identify different stock cultivars in seed production, culture and to establish cultivar certification system.

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