Carbonaceous Soot from Thermal Decomposition of Kerosene: A Pioneering Report for Paint Preservation

AbstractBacterial resistance to conventional paint biocides is widely documented. However, an investigation regarding the preservative potential of carbonaceous soot as a viable alternative is novel. Fresh paint samples (SA, SB and SC) were collected in duplicates from a reputable paint manufacturing company in Lagos, Nigeria. SA was produced with conventional paint biocide, SB and SC were without biocide. All samples were inoculated with 0.032 × 106 cfu/mL of Pseudomonas aeruginosa PA01 (NC 002516.2) previously isolated from spoilt paints and identified by the 16S rDNA analysis. SB was supplemented with crude soot (10 g/L of distilled water) at 200 mL v/v. The preservative efficiency of soot was investigated by monitoring the bacterial population (BP), optical density (OD600nm), viscosity (VIS), the transmittance (TR) and specific gravity (SG) of the samples bi-weekly for 14 weeks. BP increased drastically from 0.032-31.61 × 106 and from 0.032-112.93 × 106 in SA and SC respectively. In contrast, SB increased from 0.032-24.63 × 106 by week 4 but reduced from 24.63 – 10.95 × 106 by week 14. The OD of SA increased from 1.55 to 1.98 by week 8 and decreased to 1.74 by week 14. SB had a decrease from 1.60 to 1.49 by week 4 and increased to 1.63 by week 14, while SC increased from 1.59 to 2.54 by the 14th week.TR increased from 1.80 to 2.30 and 1.25 to 2.20 in SA and SC respectively. In contrast, TR decreased from 2.00 to 1.25 in SB. The viscosity and specific gravity values of all the samples decreased with time with the highest decrease observed in SC and the lowest in SB. The test crude soot showed preservative potential in paints.

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Mitochondrial 16S rDNA analysis used to distinguish Corbicula japonica from Japan and imported species from Korea and China

NIPPON SUISAN GAKKAISHI ◽

10.2331/suisan.76.621 ◽

2010 ◽

Vol 76 (4) ◽

pp. 621-629 ◽

Cited By ~ 4

Author(s):

AKIRA KOMARU ◽

HISAKO HORI ◽

YASUHIRO YANASE ◽

KENJI ONOUCHI ◽

TAKESHI KATO ◽

...

Keyword(s):

16S Rdna ◽

16S Rdna Analysis ◽

Corbicula Japonica ◽

Mitochondrial 16S Rdna ◽

Rdna Analysis

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Phenotyping and 16S rDNA Analysis after Biofield Treatment on Citrobacter braakii: A Urinary Pathogen

Journal of Clinical & Medical Genomics ◽

10.4172/2472-128x.1000129 ◽

2015 ◽

Vol 3 (1) ◽

Cited By ~ 2

Author(s):

Mahendra Kumar Trivedi ◽

Alice Branton

Keyword(s):

16S Rdna ◽

16S Rdna Analysis ◽

Rdna Analysis

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Identification and characterization by 16S rDNA analysis of viable bacterial colonies isolated from oral medicines based on inactivated or lysed pathogenic bacteria

Biologicals ◽

10.1016/s1045-1056(02)00091-x ◽

2003 ◽

Vol 31 (1) ◽

pp. 39-43 ◽

Cited By ~ 1

Author(s):

Edgar Studer ◽

Urs Candrian

Keyword(s):

16S Rdna ◽

Pathogenic Bacteria ◽

16S Rdna Analysis ◽

Identification And Characterization ◽

Rdna Analysis

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Bioaerosol Concentrations and the Identification of Aerosolized Bacteria by 16S rDNA Analysis in Work Environments

SANGYO EISEIGAKU ZASSHI ◽

10.1539/sangyoeisei.49.39 ◽

2007 ◽

Vol 49 (2) ◽

pp. 39-44 ◽

Cited By ~ 1

Author(s):

Sumiyo Ishimatsu ◽

Hiroki Abe ◽

Kazumasa Fukuda ◽

Toru Ishidao ◽

Hatsumi Taniguchi ◽

...

Keyword(s):

16S Rdna ◽

Work Environments ◽

16S Rdna Analysis ◽

Rdna Analysis

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Factors affecting the lytic susceptibility of some marine and terrestrial bacteria

Canadian Journal of Microbiology ◽

10.1139/m82-063 ◽

1982 ◽

Vol 28 (4) ◽

pp. 414-424 ◽

Cited By ~ 5

Author(s):

Richard A. Laddaga ◽

Robert A. MacLeod

Keyword(s):

Pseudomonas Aeruginosa ◽

Optical Density ◽

Marine Bacteria ◽

Sea Water ◽

Water Concentration ◽

Distilled Water ◽

Nacl Solution ◽

Factors Affecting ◽

E Coli ◽

Absorbing Material

Eighteen gram-negative marine bacteria and two terrestrial species, Escherichia coli and Pseudomonas aeruginosa, were examined for their sensitivity to lysis in distilled water after exposure either to a salt solution containing a sea water concentration of Mg2+ (0.05 M) or to 0.5 M NaCl. A spectrum of lytic susceptibility was observed among the marine bacteria ranging from those organisms which lysed in distilled water after exposure to the Mg2+-containing solution, through organisms which could be sensitized to lysis by washing with the NaCl solution, to organisms which failed to lyse in distilled water even after having been washed with a solution of 0.5 M NaCl. Pseudomonas aeruginosa and E. coli fell within this spectrum, the former being capable of being induced to lyse in distilled water by washing with 0.5 M NaCl, while the latter failed to lyse in distilled water after this treatment. It was thus concluded that no overall distinction could be made between marine and terrestrial bacteria on the basis of the sensitivity of the two groups of organisms to lysis in freshwater.Quite large decreases in optical density and increases in the release of ultraviolet-absorbing material took place when cells preexposed to the Mg2+-containing solution or to 0.5 M NaCl were subsequently suspended in distilled water even though in some cases no loss of cell numbers could be detected. In most cases two to three times as much K+ as Na+ and 1/10 to 1/100 as much Mg2+ was required to prevent these changes. For three of the marine bacteria and P. aeruginosa grown in a terrestrial type medium little difference in the requirements for Na+ and K+ to prevent the optical density changes was noted. For P. aeruginosa grown in a marine type medium, cells required more K+ than Na+ to prevent these changes.

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