scholarly journals In Vitro Study of Immune Properties of New Lactobacilli Isolates from Pheasant Gut

2020 ◽  
Vol 64 (1) ◽  
pp. 39-47 ◽  
Author(s):  
V. Karaffová ◽  
V. Revajová ◽  
R. Nemcová ◽  
Z. Ševčíková ◽  
M. Levkutová ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Lactobacillus Reuteri ◽  
In Vitro Study ◽  
Lymphocyte Subpopulations ◽  
Post Inoculation ◽  
Relative Percentage ◽  
Relative Expression ◽  
T Lymphocyte Subpopulations ◽  
T Cell Subpopulations

AbstractThe goal of this paper was to study the effect of Lactobacillus reuteri B1/1, B2/1 and B6/1 on the relative expression of selected interleukins (IL-1β, IL-15), macrophage inflammatory protein (MIP-1β), and the relative percentage of T lymphocyte subpopulations in peripheral mononuclear blood cells (PMBCs). The mRNA expression levels of interleukins and MIP-1β of PMBCs were evaluated at 24 h and 48 h post inoculation using the quantitative real-time polymerase chain reaction (qRT-PCR). The percentage of T lymphocyte subpopulations in PMBCs was determined by flow cytometry. The group that was administered L. reuteri B1/1 had the most significant stimulation of the expression of pro-inflammatory interleukins and MIP-1β, in particular after 24 h. Similarly, we observed a rise in the relative percentage of T cells including CD3+, CD4+ and CD8+ lymphocytes in the groups with L. reuteri B1/1 and L. reuteri B2/1. Overall, L. reuteri B1/1 and L. reuteri B2/1 showed a promising stimulatory effect on the relative expression of pro-inflammatory interleukins, MIP-1β and percentage of T cell subpopulations in vitro. On the flip side, L. reuteri B6/1 did not induce the expression of the IL-1β gene.

scholarly journals Multiple In Vitro and In Vivo Regulatory Effects of Budesonide in CD4+ T Lymphocyte Subpopulations of Allergic Asthmatics

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e48816 ◽  
Author(s):  
Elisabetta Pace ◽  
Caterina Di Sano ◽  
Stefania La Grutta ◽  
Maria Ferraro ◽  
Giuseppe Albeggiani ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Lymphocyte Subpopulations ◽  
T Lymphocyte Subpopulations ◽  
Regulatory Effects

Relapse markers in multiple sclerosis: are in vitro cytokine production changes reflected by circulatory T-cell phenotype alterations?

1998 ◽  
Vol 4 (3) ◽  
pp. 193-197 ◽  
Author(s):  
Jan Debruyne ◽  
Jan Philippé ◽  
Jacques Dereuck ◽  
Annick Willems ◽  
Geert Leroux-Roels
Keyword(s):  
T Cell ◽  
T Lymphocyte ◽  
Interleukin 2 ◽  
Lymphocyte Subpopulations ◽  
T Cell Subsets ◽  
Cell Phenotype ◽  
Monocyte Count ◽  
Serum Neopterin ◽  
T Lymphocyte Subpopulations

In vitro tumor necrosis factor-a (TNF-a), interferon-g (IFN-g) and interleukin-2 (IL-2) production, serum neopterin levels, and T-lymphocyte subpopulations were determined on a monthly basis in 22 MS patients. We found increased in vitro TNF-a production from 4 weeks on prior to the day of an exacerbation. There was a significant correlation with in vitro IFN-g release, the absolute blood monocyte count and the serum neopterin levels, suggesting that monocytes stimulated by IFN-g play an important role in the TNF-a production. Serial analysis of in vitro TNF-a production proved to be a helpful tool in predicting relapses in MS patients. Furthermore, elevated levels of IFN-g and IL-2 after stimulation with OKT3 during exacerbations were demonstrated. These increases were not reflected by changes in T-lymphocyte subpopulations. However, significant differences in T-cell subsets were observed between controls and relapsing progressive patients.

scholarly journals The production of granulocyte-monocyte colony-stimulating activity by isolated human T lymphocyte subpopulations

Blood ◽  
1984 ◽  
Vol 63 (5) ◽  
pp. 1141-1146 ◽  
Author(s):  
PJ Hesketh ◽  
R Sullivan ◽  
CR Valeri ◽  
LA McCarroll
Keyword(s):  
T Lymphocyte ◽  
Bone Marrow Cells ◽  
Lymphocyte Subpopulations ◽  
In Vitro Assays ◽  
Nonspecific Esterase ◽  
Con A ◽  
T Lymphocyte Subpopulations ◽  
Colony Stimulating Activity ◽  
Human T Lymphocyte

Abstract Isolated human T lymphocyte subpopulations were obtained by fluorescence-activated cell sorting using the murine monoclonal antibodies, OKT4 and OKT8. The capabilities of the isolated lymphocytes to produce granulocyte-monocyte colony-stimulating activity (CSA) in response to mitogen challenge were assessed by in vitro assays employing light density nonadherent bone marrow cells. Essentially, no CSA production was noted by any isolated T lymphocyte population [OKT4 positive (+) or OKT8 positive (+)] cultured alone or following the addition of 10(4) autologous monocytes/ml. When phytohemagglutinin (PHA) alone was added, OKT4+ lymphocytes elaborated small amounts of CSA. With the addition of concanavalin A (Con-A) alone, both OKT4+ and OKT8+ cells were able to produce modest amounts of CSA. Significantly enhanced CSA production was observed when either OKT4+ or OKT8+ lymphocytes were coincubated with autologous monocytes in the presence of mitogen. We conclude that highly purified T lymphocyte subpopulations, free of monocytes as assessed by nonspecific esterase staining, can elaborate small amounts of CSA in response to PHA or Con- A challenge. A synergistic augmentation of CSA production was noted with coincubation of sorted lymphocytes and autologous monocytes in the presence of mitogen. Finally, our results suggest that the ability of T lymphocytes to make CSA is not exclusively limited to either the OKT4+ or OKT8+ defined subsets.

scholarly journals The production of granulocyte-monocyte colony-stimulating activity by isolated human T lymphocyte subpopulations

Blood ◽  
1984 ◽  
Vol 63 (5) ◽  
pp. 1141-1146
Author(s):  
PJ Hesketh ◽  
R Sullivan ◽  
CR Valeri ◽  
LA McCarroll
Keyword(s):  
T Lymphocyte ◽  
Bone Marrow Cells ◽  
Lymphocyte Subpopulations ◽  
In Vitro Assays ◽  
Nonspecific Esterase ◽  
Con A ◽  
T Lymphocyte Subpopulations ◽  
Colony Stimulating Activity ◽  
Human T Lymphocyte

Isolated human T lymphocyte subpopulations were obtained by fluorescence-activated cell sorting using the murine monoclonal antibodies, OKT4 and OKT8. The capabilities of the isolated lymphocytes to produce granulocyte-monocyte colony-stimulating activity (CSA) in response to mitogen challenge were assessed by in vitro assays employing light density nonadherent bone marrow cells. Essentially, no CSA production was noted by any isolated T lymphocyte population [OKT4 positive (+) or OKT8 positive (+)] cultured alone or following the addition of 10(4) autologous monocytes/ml. When phytohemagglutinin (PHA) alone was added, OKT4+ lymphocytes elaborated small amounts of CSA. With the addition of concanavalin A (Con-A) alone, both OKT4+ and OKT8+ cells were able to produce modest amounts of CSA. Significantly enhanced CSA production was observed when either OKT4+ or OKT8+ lymphocytes were coincubated with autologous monocytes in the presence of mitogen. We conclude that highly purified T lymphocyte subpopulations, free of monocytes as assessed by nonspecific esterase staining, can elaborate small amounts of CSA in response to PHA or Con- A challenge. A synergistic augmentation of CSA production was noted with coincubation of sorted lymphocytes and autologous monocytes in the presence of mitogen. Finally, our results suggest that the ability of T lymphocytes to make CSA is not exclusively limited to either the OKT4+ or OKT8+ defined subsets.

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