Prevalence, Morphological and Molecular Phylogenetic Analyses of the Rabbit Pinworm, Passalurus ambiguus Rudolphi 1819, in the Domestic Rabbits Oryctolagus cuniculus

2019 ◽  
Vol 64 (2) ◽  
pp. 316-330 ◽  
Author(s):  
Rewaida Abdel-Gaber ◽  
Farid Ataya ◽  
Dalia Fouad ◽  
Mohamed Daoud ◽  
Shatha Alzuhairy
Keyword(s):  
Oryctolagus Cuniculus ◽  
Phylogenetic Analyses ◽  
Molecular Phylogenetic ◽  
Domestic Rabbits

Morphological and Molecular Identification of Passalurus Ambiguus Rudolphi, 1819 in Domestic Rabbits (Oryctolagus Cuniculus) in Qena Governorate, Upper Egypt

2021 ◽  
Author(s):  
Nermean Moamen Hussein ◽  
Soheir A. H. Rabie ◽  
Wafaa A. Abuelwafa ◽  
Mouchira M. Mohi ElDin
Keyword(s):  
Worm Burden ◽  
Oryctolagus Cuniculus ◽  
Intestinal Parasite ◽  
Present Species ◽  
Gastrointestinal Disorders ◽  
Upper Egypt ◽  
Worm Infestation ◽  
Molecular Phylogenetic ◽  
Domestic Rabbits ◽  
Molecular Phylogenetic Data

Abstract Domestic rabbits in Egypt are used commercially for meat, but gastrointestinal disorders can affect production. Passalurus ambiguus is an intestinal parasite that infects the rabbit causing intestinal problems and death in severe cases. The present study collected domestic rabbits from several locations tgroughout the Qena Governorate in Upper Egypt. Passalurus ambiguus worms were detected in 90 out of 200 rabbits (45%). They were described morphologically using light and scanning electron microscopy. Males measured 4.622 mm (2.838–7.172 mm) in length and 0.278 mm (0.139–0.558 mm) in width. Females measured 5.622 mm (2.347–9.532 mm) in length, 0.314 mm, and (0.185–0.381 mm) in width. Phylogenetic results confirmed the identification of the worms as Passalurus ambiguus. They appeared as small white nodules in the appendix of the rabbits examined. Histopathologically, a heavy worm burden was observed inside the appendiceal lumen, among crypts, and inside the lymphoid follicles. The heavy worm infestation leads to hyperplasia in the epithelial lining of the appendix and the follicles resulting in lumen obstruction. Granulomatous reactions were induced due to irritation and injury by the worm. It could be concluded that morphological features, molecular phylogenetic data, and histopathological findings clearly identified the present species as as Passalurus ambiguus Rudolphi, 1819.

scholarly journals Disentangling relationships among eastern Mediterranean Cymbalaria including description of a novel species from the southern Peloponnese (Greece)

2021 ◽  
Vol 307 (2) ◽  
Author(s):  
Pau Carnicero ◽  
Núria Garcia-Jacas ◽  
Llorenç Sáez ◽  
Theophanis Constantinidis ◽  
Mercè Galbany-Casals
Keyword(s):  
Eastern Mediterranean ◽  
Phylogenetic Analyses ◽  
Taxonomic Revision ◽  
Distribution Patterns ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Long Distance ◽  
Aegean Islands ◽  
Molecular Phylogenetic

AbstractThe eastern Mediterranean basin hosts a remarkably high plant diversity. Historical connections between currently isolated areas across the Aegean region and long-distance dispersal events have been invoked to explain current distribution patterns of species. According to most recent treatments, at least two Cymbalaria species occur in this area, Cymbalaria microcalyx and C. longipes. The former comprises several intraspecific taxa, treated at different ranks by different authors based on morphological data, evidencing the need of a taxonomic revision. Additionally, some populations of C. microcalyx show exclusive morphological characters that do not match any described taxon. Here, we aim to shed light on the systematics of eastern Mediterranean Cymbalaria and to propose a classification informed by various sources of evidence. We performed molecular phylogenetic analyses using ITS, 3’ETS, ndhF and rpl32-trnL sequences and estimated the ploidy level of some taxa performing relative genome size measures. Molecular data combined with morphology support the division of traditionally delimited C. microcalyx into C. acutiloba, C. microcalyx and C. minor, corresponding to well-delimited nrDNA lineages. Furthermore, we propose to combine C. microcalyx subsp. paradoxa at the species level. A group of specimens previously thought to belong to Cymbalaria microcalyx constitute a well-defined phylogenetic and morphological entity and are described here as a new species, Cymbalaria spetae. Cymbalaria longipes is non-monophyletic, but characterized by being glabrous and diploid, unlike other eastern species. The nrDNA data suggest at least two dispersals from the mainland to the Aegean Islands, potentially facilitated by marine regressions.

Infundibulicybe rufa sp. nov. (Tricholomataceae), a reddish brown species from southwestern China

Phytotaxa ◽  
2016 ◽  
Vol 266 (2) ◽  
pp. 134 ◽  
Author(s):  
QI ZHAO ◽  
YAN-JIA HAO ◽  
JIAN-KUI LIU ◽  
KEVIN D. HYDE ◽  
YANG-YANG CUI ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Biosphere Reserve ◽  
Its Region ◽  
Southwestern China ◽  
New Taxon ◽  
Line Drawings ◽  
Phylogenetic Placement ◽  
Molecular Phylogenetic ◽  
Pileus Surface

Infundibulicybe rufa sp. nov., is described from Jiuzhaigou Biosphere Reserve, southwestern China. It is characterized by the combination of the following characters: umbilicate to slightly infundibuliform, reddish brown pileus; decurrent, cream lamellae; cylindrical stipe concolorous with the pileus surface. Molecular phylogenetic analyses using the nuclear ribosomal internal transcribed spacer (ITS) region indicates that I. rufa is closely related to I. mediterranea and I. bresadolana. A description, line drawings, phylogenetic placement and comparison with allied taxa for the new taxon are presented.

Asian Betylobraconinae (Hymenoptera, Braconidae), with description of a new genus and phylogenetic affinities of the tribe Facitorini

2008 ◽  
Vol 39 (2) ◽  
pp. 133-154 ◽  
Author(s):  
Alberto Sáez ◽  
Kaoru Maeto ◽  
Alejandro Zaldivar-Riverón ◽  
Sergey Belokobylskij
Keyword(s):  
New Species ◽  
Type Species ◽  
New Genus ◽  
Phylogenetic Analyses ◽  
28S Rrna ◽  
Asian Region ◽  
Molecular Phylogenetic ◽  
Phylogenetic Affinities ◽  
One New Species ◽  
Rrna Sequences

AbstractThe taxonomy of the Asian genera of the subfamily Betylobraconinae, a small and understudied group within the hymenopteran family Braconidae, is revised. A new genus exclusively from the Asian region, Asiabregma gen. nov., containing three species (A. ryukyuensis sp. nov. (type species, Japan and Malaya), A. makiharai sp. nov. (Japan) and A. sulaensis (van Achterberg), comb. nov. (Indonesia)) is described. One new species of Aulosaphobracon, A. striatus sp. nov. from Vietnam, and one of Facitorus, F. amamioshimus sp. nov. from Japan, are also described. Based on molecular phylogenetic analyses using COI mtDNA and 28S rRNA sequences, the three genera previously placed in the tribe Facitorini, Facitorus, Conobregma and Jannya, together with Asiabregma gen. nov., are transferred to the rogadine tribe Yeliconini.

Crocinoboletus, a new genus of Boletaceae (Boletales) with unusual boletocrocin polyene pigments 

Phytotaxa ◽  
2014 ◽  
Vol 175 (3) ◽  
pp. 133 ◽  
Author(s):  
Nian-Kai Zeng ◽  
Gang Wu ◽  
Yan-Chun Li ◽  
Zhi-Qun Liang ◽  
Zhu-Liang Yang
Keyword(s):  
New Genus ◽  
Phylogenetic Analyses ◽  
Middle Part ◽  
Line Drawings ◽  
Molecular Phylogenetic ◽  
Orange Color

Crocinoboletus is described as a new genus of Boletaceae to accommodate Boletus rufoaureus and B. laetissimus, characterized by its brilliant orange color of basidiomata caused by the presence of unusual boletocrocin polyene pigments, bluish olivaceous staining of all parts when bruised, smooth basidiospores, and the pileipellis which has an interwoven trichoderm at the middle part of the pileus and a cutis at the margin of the pileus. Prior molecular phylogenetic analyses also confirmed the two taxa are not members of the genus Boletus s.s., but form a well-supported generic lineage within Boletaceae. Consequently a description, color photos of fresh basidiomata, line-drawings of microstructures and a comparison of Crocinoboletus with allied taxa are presented.

Tracing the origins of hybrids through history: monstrous cultivars and Napoléon Bonaparte’s exiled paper daisies (Asteraceae; Gnaphalieae)

2021 ◽  
Author(s):  
Timothy L Collins ◽  
Jeremy J Bruhl ◽  
Alexander N Schmidt-Lebuhn ◽  
Ian R H Telford ◽  
Rose L Andrew
Keyword(s):  
Gene Flow ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Napoleon Bonaparte ◽  
Snp Data ◽  
Cast Doubt ◽  
Molecular Phylogenetic ◽  
St Helena ◽  
New Evidence

Abstract Golden everlasting paper daisies (Xerochrysum, Gnaphalieae, Asteraceae) were some of the earliest Australian native plants to be cultivated in Europe. Reputedly a favourite of Napoléon Bonaparte and Empress Joséphine, X. bracteatum is thought to have been introduced to the island of St Helena in the South Atlantic during Napoléon’s exile there. Colourful cultivars were developed in the 1850s, and there is a widely held view that these were produced by crossing Xerochrysum with African or Asian Helichrysum spp. Recent molecular phylogenetic analyses and subtribal classification of Gnaphalieae cast doubt on this idea. Using single-nucleotide polymorphism (SNP) data, we looked for evidence of gene flow between modern cultivars, naturalized paper daisies from St Helena and four Xerochrysum spp. recorded in Europe in the 1800s. There was strong support for gene flow between cultivars and X. macranthum. Paper daisies from St Helena were genotypically congruent with X. bracteatum and showed no indications of ancestry from other species or from the cultivars, consistent with the continuous occurrence of naturalized paper daisies introduced by Joséphine and Napoléon. We also present new evidence for the origin of colourful Xerochrysum cultivars and hybridization of congeners in Europe from Australian collections.

Description of Ditylenchus acantholimonis n. sp. (Rhabditida: Anguinidae) from Iran, a morphological and molecular phylogenetic study

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Samira Aliverdi ◽  
Ebrahim Pourjam ◽  
Majid Pedram
Keyword(s):  
New Species ◽  
Rhizosphere Soil ◽  
Phylogenetic Analyses ◽  
Phylogenetic Study ◽  
Pharyngeal Bulb ◽  
Lateral Field ◽  
Molecular Phylogenetic Study ◽  
Molecular Phylogenetic ◽  
Golestan Province ◽  
Tail Tip

Summary Ditylenchus acantholimonis n. sp. is described based on morphological, morphometric and molecular characters. It was isolated from the rhizosphere soil of Acantholimon sp. in Golestan province, Iran, and is mainly characterised by having four lines in the lateral field, a pyriform to bottle-shaped offset pharyngeal bulb, post-vulval uterine sac 36.6-56.1% of the vulva to anus distance long, and a subcylindrical to conical tail with widely rounded tip. It is further characterised by short to medium-sized females, 480-617 μm long, with a fine stylet having small rounded knobs, V = 80.8-83.6, c = 11.0-13.8, c′ = 3.3-4.6, and males with 16.0-17.0 μm long spicules. The new species was morphologically compared with six species having four lines in their lateral field, rounded tail tip and comparable morphometric data namely: D. dipsacoideus, D. emus, D. exilis, D. paraparvus, D. sturhani, and D. solani. It was also compared with two species, D. ferepolitor and D. angustus, forming a maximally supported clade in the 18S tree. The phylogenetic analyses using the maximal number of Anguinidae and several Sphaerularioidea genera based upon partial 18S and 28S rDNA D2-D3 sequences revealed that Ditylenchus is polyphyletic. In the 18S tree, the new species formed a clade with D. ferepolitor (KJ636374) and D. angustus (AJ966483); in the 28S tree it formed a poorly supported clade with D. phyllobios (KT192618) and Ditylenchus sp. (MG865719).

A description of the second species of the genus Bleptus Eaton, 1885 (Ephemeroptera: Heptageniidae) from Japan, and phylogenetic relationships of two Bleptus mayflies inferred from mitochondrial and nuclear gene sequences

Zootaxa ◽  
2021 ◽  
Vol 4974 (2) ◽  
pp. 333-360
Author(s):  
KOJI TOJO ◽  
KEN MIYAIRI ◽  
YUTO KATO ◽  
AYANA SAKANO ◽  
TOMOYA SUZUKI
Keyword(s):  
Phylogenetic Analyses ◽  
Ribosomal Subunit ◽  
Nuclear Gene ◽  
Taxonomic Status ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Gene Sequences ◽  
Molecular Phylogenetic ◽  
Black Band ◽  
Relationship Of

A new mayfly species, Bleptus michinokuensis sp. nov. (Ephemeroptera: Heptageniidae) is described on the basis of specimens of male and female adults and mature nymphs collected at a seepage zone of a small freshwater branch of the ‘Tachiya-zawa-gawa’ River located amongst the northern foothills of Mt. Gassan (Shonai-machi Town, Yamagata Prefecture, Japan). This new Bleptus species is characterized by its clear fore and hind wings. That is, they neither exhibit the distinct black band on the fore wings, nor the characteristic darkened margins along the edges of both the fore and hind wings. Rather it has a blackish colored terminal half of its fore legs (i.e., tibial, tarsal and pretarsal segments). These features differ clearly when comparing them to the other known species, Bleptus fasciatus Eaton. The information and data describing the habitat and distribution range of this new species are also noted. We also examined and discussed the genetic relationship of two Bleptus mayflies to settle the taxonomic status, inferred from the partially sequenced cytochrome c oxidase subunit I (COI) and large mitochondrial ribosomal subunit (16S rRNA) genes, and also the nuclear internal transcribed spacer 2 (ITS2) gene sequences. Consequently, phenetic and molecular phylogenetic analyses agreed well in terms of clustering. 

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