Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-α DNA sequences and 25S LSU rRNA sequences

Biologia ◽  
2013 ◽  
Vol 68 (5) ◽  
Author(s):  
Peter Pristas ◽  
Svetlana Gaperova ◽  
Jan Gaper ◽  
Jana Judova
Keyword(s):  
Genetic Variability ◽  
Cryptic Species ◽  
Dna Sequences ◽  
Elongation Factor ◽  
Its Sequence ◽  
Sequence Variability ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Fomes Fomentarius ◽  
Elongation Factor 1

AbstractThe existence of two cryptic species within strains of the wood-decaying fungus Fomes fomentarius was revealed recently based on the internal transcribed spacer (ITS) sequence variability. In this study for the first time the sequences of another molecular markers, partial translation elongation factor 1-α (efa) region and partial 25S large subunit ribosomal RNA gene were obtained and used to evaluate genetic variability of F. fomentarius. Congruent phylogeny was observed for all three markers used confirming the presence of two cryptic species within F. fomentarius. Surprisingly, ITS sequence variability within F. fomentarius was significantly lower compared to the variability of efa sequences (0.023 versus 0.036 nucleotide substitutions per site) questioning the discriminatory power of ITS sequences for fungal species identification.

scholarly journals New species of Retiboletus (Boletales, Boletaceae) from China based on morphological and molecular data

MycoKeys ◽  
2020 ◽  
Vol 67 ◽  
pp. 33-44
Author(s):  
Hai-Ying Liu ◽  
Yan-Chun Li ◽  
Tolgor Bau
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Large Subunit ◽  
Elongation Factor ◽  
Molecular Data ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Line Drawings ◽  
Morphological And Molecular Data ◽  
Elongation Factor 1

Species of the genus Retiboletus in China were investigated based on morphology and phylogenetic analysis of DNA sequences from the nuclear ribosomal large subunit (nrLSU) and the translation elongation factor 1-α gene (TEF1-α). Nine species were recovered from China, including two new and seven known species. The new species, namely Retiboletus ater and R. sinogriseus, from southwestern and northeastern China respectively, are documented and illustrated in this paper. Retiboletus ater is morphologically characterized by its black to grayish black pileus, white to grayish hymenophore, black to blackish stipe and white to grayish white context. Retiboletus sinogriseus is morphologically characterized by its brown to grayish-brown pileus, yellow to grayish-yellow hymenophore, pale yellow to brownish stipe and yellow to brownish-yellow context. Descriptions and line drawings of these two novel species and their comparisons with allied taxa are presented.

scholarly journals Survey, Identification, and Characterization of Cylindrocarpon-Like Asexual Morphs in Spanish Forest Nurseries

Plant Disease ◽  
2018 ◽  
Vol 102 (11) ◽  
pp. 2083-2100 ◽  
Author(s):  
Beatriz Mora-Sala ◽  
Ana Cabral ◽  
Maela León ◽  
Carlos Agustí-Brisach ◽  
Josep Armengol ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Elongation Factor ◽  
Its Region ◽  
Phylogenetic Position ◽  
Translation Elongation ◽  
Forest Nurseries ◽  
Asexual Morphs ◽  
Identification And Characterization ◽  
Elongation Factor 1

Cylindrocarpon-like asexual morphs infect herbaceous and woody plants, mainly in agricultural scenarios, but also in forestry systems. The aim of the present study was to characterize a collection of Cylindrocarpon-like isolates recovered from the roots of a broad range of forest hosts from nurseries showing decline by morphological and molecular studies. Between 2009 and 2012, 17 forest nurseries in Spain were surveyed and a total of 103 Cylindrocarpon-like isolates were obtained. Isolates were identified based on DNA sequences of the partial gene regions histone H3 (his3). For the new species, the internal transcribed spacer and intervening 5.8S nrRNA gene (ITS) region, β-tubulin (tub2), and translation elongation factor 1-α (tef1) were also used to determine their phylogenetic position. Twelve species belonging to the genera Cylindrodendrum, Dactylonectria, and Ilyonectria were identified from damaged roots of 15 different host genera. The species C. alicantinum, D. macrodidyma, D. novozelandica, D. pauciseptata, D. pinicola, D. torresensis, I. capensis, I. cyclaminicola, I. liriodendri, I. pseudodestructans, I. robusta, and I. rufa were identified. In addition, two Dactylonectria species (D. hispanica sp. nov. and D. valentina sp. nov.), one Ilyonectria species (I. ilicicola sp. nov.), and one Neonectria species (N. quercicola sp. nov.) are newly described. The present study demonstrates the prevalence of this fungal group associated with seedlings of diverse hosts showing decline symptoms in forest nurseries in Spain.

Sequence of a cDNA encoding the α-subunit of wheat translation elongation factor 1

Gene ◽  
1992 ◽  
Vol 120 (2) ◽  
pp. 315-316 ◽  
Author(s):  
Anneke M. Metz ◽  
Richard T. Timmer ◽  
M.Leah Allen ◽  
Karen S. Browning
Keyword(s):  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Α Subunit ◽  
Elongation Factor 1

scholarly journals Global Molecular Diversity of the Halotolerant Fungus Hortaea werneckii

Life ◽  
2018 ◽  
Vol 8 (3) ◽  
pp. 31 ◽  
Author(s):  
Alessia Marchetta ◽  
Bert Gerrits van den Ende ◽  
Abdullah Al-Hatmi ◽  
Ferry Hagen ◽  
Polona Zalar ◽  
...  
Keyword(s):  
Molecular Diversity ◽  
Sea Water ◽  
Elongation Factor ◽  
Clonal Expansion ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Hortaea Werneckii ◽  
Halotolerant Fungus ◽  
Elongation Factor 1 ◽  
Partial Translation

A global set of clinical and environmental strains of the halotolerant black yeast-like fungus Hortaea werneckii are analyzed by multilocus sequencing and AFLP, and physiological parameters are determined. Partial translation elongation factor 1-α proves to be suitable for typing because of the presence/absence of introns and also the presence of several SNPs. Local clonal expansion could be established by a combination of molecular methods, while the population from the Mediterranean Sea water also responds differently to combined temperature and salt stress. The species comprises molecular populations, which in part also differ physiologically allowing further diversification, but clinical strains did not deviate significantly from their environmental counterparts.

Diaporthe sinensis, a new fungus from Amaranthus sp. in China 

Phytotaxa ◽  
2019 ◽  
Vol 425 (5) ◽  
pp. 259-268
Author(s):  
XIAO-XIAO FENG ◽  
JIA-JIE CHEN ◽  
GUO-RONG WANG ◽  
TING-TING CAO ◽  
YONG-LI ZHENG ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Plant Pathogens ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Fungal Species ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Stems And Leaves ◽  
Elongation Factor 1 ◽  
Β Tubulin

During an exploration of plant pathogens in vegetables occuring in Zhejiang province, China, a novel fungal species, was found. Three strains ZJUP0033-4, ZJUP0038-3 and ZJUP0132 were isolated from black round lesions in the stems and leaves of Amaranthus sp. Phylogenetic analyses based on sequences from four genes including rDNA internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α), histone (HIS) and β-tubulin (TUB) indicated that D. sinensis clustered in a distinct clade closely related to D. neoarctii, D. angelicae, D. subordinaria, D. arctii, D. cuppatea, D. lusitanicae, D. novem, D. infecunda, D. ganjae and D. manihotia. Morphologically, D. sinensis is distinguished by brown, scattered, globose pycnidia and ellipsoid alpha conidia with bi- to multiguttulate.

Additions to pestalotioid fungi in Thailand: Neopestalotiopsis hydeana sp. nov. and Pestalotiopsis hydei sp. nov.

Phytotaxa ◽  
2021 ◽  
Vol 479 (1) ◽  
pp. 23-43
Author(s):  
NARUEMON HUANALUEK ◽  
RUVISHIKA S. JAYAWARDENA ◽  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
DULANJALEE L. HARISHCHANDRA
Keyword(s):  
Elongation Factor ◽  
Climatic Conditions ◽  
Annona Squamosa ◽  
Translation Elongation Factor ◽  
Garcinia Mangostana ◽  
Translation Elongation ◽  
Leaf Spots ◽  
First Time ◽  
Elongation Factor 1 ◽  
Partial Translation

Pestalotioid fungi commonly occur as pathogens, endophytes or saprobes. In this study, pestalotioid fungi associated with leaf spots and fruit rots were isolated from Alpinia malaccensis, A. galangal, Annona squamosa, Artocarpus heterophyllus, Citrus sp., Garcinia mangostana, Litsea petiolata, a pteridophyte, and Vitis vinifera in Chiang Rai, Thailand. Based on single- and multi-locus phylogenies using internal transcribed spacer, β-tubulin and partial translation elongation factor 1-α gene regions, along with morphological features, the isolates were identified as two new species, Neopestalotiopsis hydeana and Pestalotiopsis hydei. This is the first time a Pestalotiopsis sp. was reported associated with Litsea petiolata and a Neopestalotiopsis sp. recorded from Alpinia, Annona, Artocarpus, Garcinia and a pteridophyte in the world. This fungal group can be considered as an emerging pathogenic group on different hosts in different climatic conditions. 

scholarly journals Translation elongation factor 1-α gene as a potential taxonomic and identification marker in dermatophytes

2014 ◽  
Vol 53 (3) ◽  
pp. 215-224 ◽  
Author(s):  
Hossein Mirhendi ◽  
Koichi Makimura ◽  
G. Sybren de Hoog ◽  
Ali Rezaei-Matehkolaei ◽  
Mohammad Javad Najafzadeh ◽  
...  
Keyword(s):  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1
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