Self-incompatibility in passion fruit: cellular responses in incompatible pollinations

Biologia ◽  
2014 ◽  
Vol 69 (5) ◽  
Author(s):  
Hérika Madureira ◽  
Telma Pereira ◽  
Maura Cunha ◽  
Denise Klein ◽  
Marcos Oliveira ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Structural Changes ◽  
Membrane System ◽  
Cellular Responses ◽  
Pollen Grain ◽  
Passion Fruit ◽  
Self Incompatibility ◽  
Passiflora Edulis ◽  
Incompatible Pollination ◽  
Compatible Interactions

AbstractSelf-incompatibility (SI) is a genetic mechanism in angiosperms that prevents selfing. The SI system in passion fruit (Passiflora edulis Sims) was investigated using hand pollinations. Pollen tube growth was inspected by microscopy, and sequence analysis of potential regulators of this process was carried out. The results revealed that the pollen tubes grew slowly and were often completely arrested in the stigma in an incompatible combination. Under these circumstances the pollen tube was rapidly and significantly rearranged, followed by the rapid deposition of callose in the stigma during the SI response. The structural changes in the pollen grain after an incompatible pollination were investigated using scanning electron microscopy. Furthermore, ultrastructural observations during incompatible interactions showed that the membrane system of the pollen tube was damaged, and fertilisation was not observed or was considerably delayed when compared to compatible interactions. The analysis presented here provides evidence that the passion fruit genome presents similar sequences to those encoding factors involved in SI in different species. These results suggest that, in the SI system of passion fruit, the rejection of an incompatible pollen grain is characterised by drastic structural changes in both pollen and pollen tube.

SELF-INCOMPATIBILITY IN PASSION FRUIT (PASSIFLORA EDULIS SIMS)

1995 ◽  
pp. 45-58 ◽  
Author(s):  
C.H. Bruckner ◽  
V.W.D. Casali ◽  
C.F. de Moraes ◽  
A.J. Regazzi ◽  
E.A.M. da Silva
Keyword(s):  
Passion Fruit ◽  
Self Incompatibility ◽  
Passiflora Edulis ◽  
Passiflora Edulis Sims

The RSS system of unidirectional cross-incompatibility in maize. 2. Cytology

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 560-564
Author(s):  
Abdul Rashid ◽  
Peter A. Peterson
Keyword(s):  
Pollen Tube ◽  
Female Parent ◽  
Pollen Tubes ◽  
Pollen Grain ◽  
Maize Pollen ◽  
Transmitting Tract ◽  
Incompatible Pollination ◽  
Recessive Genes ◽  
Maize Genetics ◽  
Incompatibility Reaction

In 1975, a number of genetic lines discovered in our maize genetics nursery in Ames, Iowa, showed unidirectional cross-incompatibility. Later, it was found that this unidirectional cross-incompatibility is controlled by three recessive genes. One locus (cif) controls the incompatibility reaction in the female tissue and the other two (cim1 and cim2) control the incompatibility reaction in the pollen grain. The cross is incompatible only when the female parent is homozygous recessive for the cif and the male parent is homozygously recessive for the cim1 as well as the cim2 locus. Cytological studies of this unidirectional cross-incompatibility show that the site of the incompatibility reaction occurs after the entry of the pollen tubes into the transmitting tract of the incompatible silks. Between 12 and 24 h after pollination, the incompatible pollination is characterized by the swelling and bursting of pollen tubes at the tip, after which pollen tube growth stops.Key words: maize, pollen tube, cross-incompatibility.

scholarly journals Development of pollen grain in yellow passion-fruit (Passiflora edulis f. flavicarpa; Passifloraceae)

2000 ◽  
Vol 23 (2) ◽  
pp. 469-473 ◽  
Author(s):  
Margarete Magalhães de Souza ◽  
Telma Nair Santana Pereira
Keyword(s):  
Male Gamete ◽  
Optical Microscope ◽  
Pollen Grain ◽  
Passion Fruit ◽  
Normal Pattern ◽  
Passiflora Edulis ◽  
Grain Formation ◽  
Safranin O ◽  
Mother Cells ◽  
Yellow Passion Fruit

To clarify events occurring during pollen grain formation in yellow passion-fruit (Passiflora edulis f. flavicarpa), floral buds were collected at different stages of development. After bracket, petal and sepal removal the anthers were fixed, dehydrated, embedded in paraffin wax, sectioned at 10 mum and after differential dying with safranin O and fast green, mounted in Canada balsam and observed under optical microscope. Formation of the male gamete followed the normal pattern for angiosperms. Observation covered final sporogenic mass phase up to pollen grain formation; microsporangium tissue modifications were also observed. Microsporogenesis was characterized by sporogenic tissue differentiation in microsporic mother cells, followed by meiosis and resulting in tetrads. Microgametogenesis began with callose microspore release, subsequent mitosis, in addition to radial and tangential tapetum wall degradation, parietal layer compression nearer to the tapetum and endothecium widening, terminating in mature pollen grain formation.

Incompatibility in Petunia

1975 ◽  
Vol 188 (1092) ◽  
pp. 299-311 ◽  
Keyword(s):  
Pollen Tube ◽  
De Novo ◽  
Qualitative Difference ◽  
Structural Proteins ◽  
Self Incompatibility ◽  
Incompatibility System ◽  
Rna And Protein Synthesis ◽  
Incompatible Pollination ◽  
Incompatibility Barrier

Petunia has a monofactorial gametophytic self-incompatibility system which is characterized by a mechanism for pollen-tube rejection after incompatible pollination about half-way between stigma and ovule. From in vitro experiments it is known that pollen tube extension requires de novo synthesis of proteins on 80 S ribosomes. The synthesis of these structural proteins has no need of new mRNA formation. The situation in the style is quite different: both RNA and protein synthesis are positively affected by pollination. There is a qualitative difference in synthesis between self- and cross-pollinated styles. These findings suggest that inhibition or rejection of pollen tubes is ‘the normal reaction’, whereas unaffected penetration is based on the synthesis of special enzymes after compatible pollination which break down the existing incompatibility barrier.

scholarly journals Biological fixation, transfer and balance of nitrogen in passion fruit (Passiflora edulis Sims) orchard intercropped with different green manure crops

2019 ◽  
Vol 13 ((03) 2019) ◽  
pp. 465-471 ◽  
Author(s):  
Edilândia Farias Dantas ◽  
Ana Dolores Santiago de Freitas ◽  
Maria do Carmo Catanho Pereira de Lyra ◽  
Carolina Etienne de Rosália e Silva Santos ◽  
Stella Jorge de Carvalho Neta ◽  
...  
Keyword(s):  
Cover Crops ◽  
Pigeon Pea ◽  
Passion Fruit ◽  
Biological Fixation ◽  
Mineral Fertilization ◽  
Passiflora Edulis ◽  
Jack Bean ◽  
Positive Balance ◽  
Sunn Hemp ◽  
Passiflora Edulis Sims

Green manures can replace or supplement mineral fertilization and add organic matter to the soils, ensuring greater sustainability to fruit growing in semiarid regions. Biological fixation, transfer and balance of nitrogen were determined on an irrigated yellow passion fruit orchard (Passiflora edulis Sims) intercropped separately with three cover crops: sunn hemp, Crotalaria juncea (L.); pigeon pea, Cajanus cajan (L.) Mill; and jack bean, Canavalia ensiformis (L.) DC. In a fourth treatment, legumes were not planted, but spontaneous vegetation was left to grow freely. The legumes were croped for 90 days in three lines (0.5 m apart) inside the passion fruit plant lines (2.5 m apart). Fixation and transfers were determined by the 15N natural abundance technique, using sunflower as a reference plant. The three planted legumes nodulated abundantly and fixed nitrogen in high proportions (between 50 and 90% of their N), forming symbiosis with bacteria naturally established in the soil. Jack bean produced more biomass than sunn hemp and pigeon pea, and as much as the spontaneous plants, of which 23% were legumes. The amounts of fixed N (150, 43, 30 and 29 kg ha-1) were determined mainly by the biomass of legumes. More than 40% of the N of passion fruit plants came from the biological nitrogen fixation of the intercropped jack bean, which provided an amount of N higher than that exported in the fruits, generating a positive balance of more than 100 kg ha-1. Therefore, it is recommended to intercrop jack bean in irrigated passion fruit orchards.

scholarly journals Genetic Diversity of Purple Passion Fruit, Passiflora edulis f. edulis, Based on Single-Nucleotide Polymorphism Markers Discovered through Genotyping by Sequencing

Diversity ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 144
Author(s):  
Nohra Castillo Rodríguez ◽  
Xingbo Wu ◽  
María Isabel Chacón ◽  
Luz Marina Melgarejo ◽  
Matthew Wohlgemuth Blair
Keyword(s):  
Genetic Diversity ◽  
Single Nucleotide Polymorphism ◽  
Genotyping By Sequencing ◽  
Passion Fruit ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Passiflora Edulis ◽  
Tropical Fruit ◽  
Commercial Cultivars ◽  
Purple Passion Fruit

Orphan crops, which include many of the tropical fruit species used in the juice industry, lack genomic resources and breeding efforts. Typical of this dilemma is the lack of commercial cultivars of purple passion fruit, Passiflora edulis f. edulis, and of information on the genetic resources of its substantial semiwild gene pool. In this study, we develop single-nucleotide polymorphism (SNP) markers for the species and show that the genetic diversity of this fruit crop has been reduced because of selection for cultivated genotypes compared to the semiwild landraces in its center of diversity. A specific objective of the present study was to determine the genetic diversity of cultivars, genebank accession, and landraces through genotyping by sequencing (GBS) and to conduct molecular evaluation of a broad collection for the species P. edulis from a source country, Colombia. We included control genotypes of yellow passion fruit, P. edulis f. flavicarpa. The goal was to evaluate differences between fruit types and compare landraces and genebank accessions from in situ accessions collected from farmers. In total, 3820 SNPs were identified as informative for this diversity study. However, the majority distinguished yellow and purple passion fruit, with 966 SNPs useful in purple passion fruits alone. In the population structure analysis, purple passion fruits were very distinct from the yellow ones. The results for purple passion fruits alone showed reduced diversity for the commercial cultivars while highlighting the higher diversity found among landraces from wild or semi-wild conditions. These landraces had higher heterozygosity, polymorphism, and overall genetic diversity. The implications for genetics and breeding as well as evolution and ecology of purple passion fruits based on the extant landrace diversity are discussed with consideration of manual or pollinator-assisted hybridization of this species.

scholarly journals Testing of self-(in)compatibility in apricot cultivars from European breeding programmes

2013 ◽  
Vol 40 (No. 2) ◽  
pp. 65-71 ◽  
Author(s):  
D. Milatović ◽  
D. Nikolić ◽  
B. Krška
Keyword(s):  
Fluorescence Microscopy ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Reliable Method ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Breeding Programmes

Self-(in)compatibility was tested in 40 new apricot cultivars from European breeding programmes. Pollen-tube growth in pistils from laboratory pollinations was analysed using the fluorescence microscopy. Cultivars were considered self-compatible if at least one pollen tube reached the ovary in the majority of pistils. Cultivars were considered self- incompatible if the growth of pollen tubes in the style stopped along with formation of characteristic swellings. Of the examined cultivars, 18 were self-compatible and 22 were self-incompatible. Fluorescence microscopy provides a relatively rapid and reliable method to determine self-incompatibility in apricot cultivars.      

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