scholarly journals POTENSI TIGA ISOLAT BAKTERI INDIGEN DARI KABUPATEN SIAK PROVINSI RIAU DALAM MENDEGRADASI NAFTALENA

Jurnal Kimia ◽  
2020 ◽  
pp. 94
Author(s):  
R. Novianty ◽  
B. Antika ◽  
. Saryono ◽  
A. Awaluddin ◽  
N. W. Pratiwi
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Optical Density ◽  
Aromatic Hydrocarbons ◽  
Growth Response ◽  
Final Concentration ◽  
Pseudomonas Sp ◽  
Joint Operation ◽  
Polycyclic Aromatic ◽  
Degradation Ability

Naphthalene is a group of Polycyclic Aromatic Hydrocarbons (PAHs) which is carcinogenic when presents in the environment. The purpose of this study was to determine the growth response and test the effectiveness of three isolates of indigen bacteria from the Joint Operation Agency area of ??PT. Siak Bumi Pusako-Pertamina Hulu (BOB BSP-PHE) in degrading naphthalene. The three isolates were tested in a Minimum Media (MM) liquid containing naphthalene with a final concentration of 0.2 mM and was incubated for 7 days. Optical Density (OD) and the degradation ability were analyzed using a UV-Vis spectrophotometer. The results showed that Pseudomonas sp. LBKURCC149 was able to grow in media containing naphthalene. Naphthalene is used as the only source of carbon and is able to degrade naphthalene by 21.98%.   Keywords: biodegradation, indigenous, naphthalene, Pseudomonas sp.

Degradation of polycyclic aromatic hydrocarbons and aromatic heterocycles by a Pseudomonas species

1988 ◽  
Vol 34 (10) ◽  
pp. 1135-1141 ◽  
Author(s):  
J. M. Foght ◽  
D. W. S. Westlake
Keyword(s):  
Gas Chromatography ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Capillary Gas Chromatography ◽  
Pseudomonas Sp ◽  
Aromatic Heterocycles ◽  
Wide Range ◽  
Pseudomonas Species ◽  
Polycyclic Aromatic ◽  
Cryptic Plasmids

Enrichment cultures were established with the aromatic fraction of a crude oil and screened for aromatic-degrading pseudomonads, using a sprayed plate technique. One isolate identified as Pseudomonas sp. HL7b was chosen for further study because it oxidized several polycyclic aromatic hydrocarbons and aromatic heterocycles without an apparent lag. Using capillary gas chromatography, spectrophotometry, and radiorespirometry, it was found to be capable of mineralizing and (or) oxidizing a wide range of polycyclic aromatic hydrocarbons, S-, N-, and O-heterocyclic analogues, and alkyl polycyclic aromatic hydrocarbons, but not aliphatic hydrocarbons. The isolate displayed two colonial morphologies which correlated with variation in degradative phenotype and hydrophobicity as measured by polystyrene adherence. Four cryptic plasmids were observed in both colonial types. Pseudomonas sp. HL7b degraded dibenzothiophene co-metabolically by a recognized pathway, but this degradation was constitutive, rather than inducible as reported for other bacteria.

Identification of A Ring-Hydroxylating Dioxygenases Capable of Anthracene and Benz[a]anthracene Oxidization from Rhodococcus sp. P14

2018 ◽  
Vol 28 (4) ◽  
pp. 183-189 ◽  
Author(s):  
Tao Peng ◽  
An Luo ◽  
Jie Kan ◽  
Lei Liang ◽  
Tongwang Huang ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Carbon Source ◽  
Recombinant Protein ◽  
Aromatic Hydrocarbons ◽  
E Coli ◽  
Pah Degradation ◽  
The World ◽  
Polycyclic Aromatic ◽  
Single Carbon Source ◽  
Degradation Ability

Nowadays, contamination of soil and marine sediments by polycyclic aromatic hydrocarbons (PAHs) has become a serious problem all over the world. <i>Rhodococcus</i> sp. P14 was isolated from sediments with crude oil contaminate and showed degradation ability on various PAHs. The genome of <i>Rhodococcus</i> sp. P14 was sequenced. A gene cluster encoding a ring-hydroxylating dioxygenase Baa related to PAH degradation was identified by bioinformatics. The expression level of gene <i>baaA</i> was increased when P14 was cultured with anthracene, pyrene, phenanthrene, or benz[a]­anthracene as the single carbon source. The recombinant protein Baa was overexpressed in <i>E. coli</i> BL21 (DE3). Further investigations on the recombinant protein Baa in <i>E. coli</i> demonstrated that it was able to oxidize anthracene and benz [a]anthracene, resulting in 9,10-dihydroxyanthracene and 7, 12-dihydroxybenz[a]anthracene as metabolites, respectively. These results indicate that Baa plays an important role in PAH degradation in <i>Rhodococcus</i> sp. P14 and Baa has potential application in the bioremediation of PAHs in the contaminated environment.

The Assessment of Contamination and Source of Polycyclic Aromatic Hydrocarbons from the Sediments of the Someş River

2019 ◽  
Vol 64 (1) ◽  
pp. 55-67
Author(s):  
Vlad Pӑnescu ◽  
◽  
Mihaela Cӑtӑlina Herghelegiu ◽  
Sorin Pop ◽  
Mircea Anton ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Polycyclic Aromatic
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