scholarly journals Sputum as a source of adipokines in bronchial asthma

2014 ◽  
Vol 21 (3) ◽  
pp. 34-40
Author(s):  
V. N. Mineev ◽  
T. M. Lalaeva ◽  
T. S. Vasiljeva ◽  
A. A. Kuzmina
Keyword(s):  
Blood Plasma ◽  
Bronchial Asthma ◽  
Immunosorbent Assay ◽  
Respiratory Function ◽  
Target Organ ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bronchial Secretion ◽  
Controlled System ◽  
Anti Inflammatory

Forty-four patients with allergic (ABA) and non-allergic (NABA) variants of bronchial asthma (BA) were examined to evaluate levels of key adipokines (leptin, resistin, adiponectin) in sputum in different variants of BA. Adipokines in sputum and blood plasma were measured by Enzyme-Linked Immunosorbent Assay (ELISA). The indices that reflect the percentage of adipokines in sputum regarding adipokines in plasma of the same patients were worked out to evaluate the ratio of levels of corresponding adipokines in plasma and sputum in patients with BA. Two regularities are clearly seen in the study: the first - levels of proinflammatory adipokines (leptin, resistin) in sputum in ABA correlate directly with indicators of respiratory function but levels of anti-inflammatory adipokines (adiponectin) in sputum correlate inversely with indicators of respiratory function; the second -correlation of levels of the studied adipokines with indicators of respiratory function are almost not revealed in NABA. The first regularity reflects the important fact that the content of adipokines in bronchial secretion is to a certain extent one of regulating local mechanisms in target organ controlled system levels of corresponding adipokines in exacerbation of BA.

scholarly journals GASTROESOPHAGEAL REFLUX DISEASE AND BRONCHIAL ASTHMA: MECHANISMS OF COMORBID FORMATION AND COURSE.

2020 ◽  
Vol 2 ◽  
pp. 3-7
Author(s):  
Aleksey Oparin ◽  
Givi Akhvlediani ◽  
Anatoliy Oparin ◽  
Sergei Pavlov
Keyword(s):  
Bronchial Asthma ◽  
Interleukin 4 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clear Correlation ◽  
The Third ◽  
Gender And Age ◽  
Galectin 3 ◽  
Degree Of Certainty ◽  
Anti Inflammatory

The aim of the study: To estimate the level of Galectin-3 with the parallel tracing of the content of pro- and anti-inflammatory cytokines Interleukin-6 (IL-6) and Interleukin-4 (IL-4) for patients with GERD and BA both in case of separate nosologies, and in case of their combined course during the period of exacerbation of the diseases. Methods. The study was conducted in three groups of patients, homogeneous by gender and age. The first group included 18 patients with GERD. The second group included 19 patients with intermittent or persistent-mild bronchial asthma. The third group included 22 patients suffering from GERD with concomitant BA intermittent or persistent-mild severity. Determination of the level of galectin-3 and interleukins (IL-4 and IL-6) in the blood serum was carried out by enzyme-linked immunosorbent assay. Results. Analyzing the results of the study, we found that the level of galectin - 3 was increased on average in both groups of patients with isolated GERD (and in patients with BA). In patients of the third group with comorbid pathology, the level of galectin-3 was statistically significantly higher than not only the norm, but also the average of patients with isolated BA and GERD. At the same time, we found the rise in the level of pro-inflammatory (IL-6) and anti-inflammatory (IL-4) cytokines. Moreover, in patients with GERD, the level of IL-6 was increased with a higher degree of reliability, and the level of IL-4 was increased with a lower degree of reliability. In patients with BA, on the contrary, the level of IL-4 was determined more often and higher, and the level of IL-6 was lower. Conclusions. Analyzing result of the study, a clear correlation and features of changes in the level of galectin-3, IL-4, IL-6 in patients with isolated GERD, BA, as well as with the comorbidity of these diseases, were revealed. In patients with BA, the level of galectin-3 increases with the same degree of certainty as in the group of patients with GERD. In the cytokine system, on the contrary, the level of anti-inflammatory (IL-4) cytokines increases with a greater degree of certainty than the level of pro-inflammatory (IL-6) cytokines. In patients with GERD with concomitant BA, the level of galectin-3 increases with a greater degree of certainty. It is observed also a higher rising of pro-inflammatory (IL-6) cytokines and a slightly pronounced increasing of the level of anti-inflammatory (IL-4) cytokines in comparison with the group of patients with isolated GERD.

scholarly journals One New Conjugate of a Secoiridoid Glucoside with a Sesquiterpene Glucoside from the Flower Buds of Lonicera japonica

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000
Author(s):  
Biao Yang ◽  
Zhaoqing Meng ◽  
Yimin Ma ◽  
Zhenzhong Wang ◽  
Gang Ding ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
2D Nmr ◽  
Lonicera Japonica ◽  
Enzyme Linked Immunosorbent Assay ◽  
Flower Buds ◽  
Spectroscopic Analyses ◽  
Anti Inflammatory ◽  
Secoiridoid Glucoside ◽  
Secoiridoid Derivatives

Secosesquside (1), a new secoiridoid glucoside-sesquiterpene conjugate, together with three known secoiridoid derivatives, were isolated from flower buds of Lonicera japonica. The isolated compounds were elucidated by extensive spectroscopic analyses, especially 2D NMR experiments. The anti-inflammatory activities of the new compound were also evaluated by enzyme-linked immunosorbent assay.

scholarly journals DETEKSI Streptococcus Sp PADA Clarias gariepinus MENGGUNAKAN AMPLIFIED ENZYME-LINKED IMMUNOSORBENT ASSAY

1999 ◽  
Vol 5 (1) ◽  
pp. 23
Author(s):  
Sri P. Astuti Wahyuningsih ◽  
R. Warsito ◽  
Hastari Wuryastuti ◽  
Kamiso H.N.
Keyword(s):  
Immunosorbent Assay ◽  
Clarias Gariepinus ◽  
Target Organ ◽  
Enzyme Linked Immunosorbent Assay ◽  
Primary Target ◽  
Kidney Liver ◽  
Diagnosis Approach ◽  
Elisa Result

An enzyme-linked immunosorbent assay has been developed which detect Streptococcus sp. Twenty Clarian gariepinus at the age of two weeks was soaked in Streptococcus sp. suspension with concentration of 108 bacteria per ml for two hours. Specimens such as blood, mucous, muscles, heart, kidney, liver, intestines and gill were collected, and assayed for the presence of Steptococcus sp. using ELISA. Result of present study showed that ELISA can be applied to detect an isolated Streptococcus sp. from tissues of Clarias gariepinus. Kidney is a primary target organ (predilection). Therefore, the kidney is a specimen of choice for diagnosis approach (es) of Streptococcus sp. infection.

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