Using Bioinformatics and Molecular Visualization to Develop Student Hypotheses in a Malate Dehydrogenase Oriented CURE

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

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Investigation of the relation of the pH-dependent dissociation of malate dehydrogenase to modification of the enzyme by N-ethylmaleimide.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)40025-1 ◽

1977 ◽

Vol 252 (17) ◽

pp. 6038-6041 ◽

Cited By ~ 2

Author(s):

C T Hodges ◽

J C Wiggins ◽

J H Harrison

Keyword(s):

Malate Dehydrogenase ◽

Ph Dependent

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Four Weeks of Aerobic Training Affects Cardiac Tissue Matrix Metalloproteinase, Lactate Dehydrogenase and Malate Dehydrogenase Enzymes Activities, and Hepatorenal Biomarkers in Experimental Hyperhomocysteinemia in Rats

International Journal of Molecular Sciences ◽

10.3390/ijms22136792 ◽

2021 ◽

Vol 22 (13) ◽

pp. 6792

Author(s):

Dusan Todorovic ◽

Marija Stojanovic ◽

Ana Medic ◽

Kristina Gopcevic ◽

Slavica Mutavdzin ◽

...

Keyword(s):

Physical Activity ◽

Lactate Dehydrogenase ◽

Malate Dehydrogenase ◽

Subcutaneous Injection ◽

Cardiac Tissue ◽

Albino Rats ◽

Wistar Albino Rats ◽

And Control ◽

Tissue Matrix ◽

Increased Activity

The aim of this study was to investigate the effect of the application of homocysteine as well as its effect under the condition of aerobic physical activity on the activities of matrix metalloproteinases (MMP), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in cardiac tissue and on hepato-renal biochemical parameters in sera of rats. Male Wistar albino rats were divided into four groups (n = 10, per group): C: 0.9% NaCl 0.2 mL/day subcutaneous injection (s.c.); H: homocysteine 0.45 µmol/g b.w./day s.c.; CPA saline (0.9% NaCl 0.2 mL/day s.c.) and a program of physical activity on a treadmill; and HPA homocysteine (0.45 µmol/g b.w./day s.c.) and a program of physical activity on a treadmill. Subcutaneous injection of substances was applied 2 times a day at intervals of 8 h during the first two weeks of experimental protocol. Hcy level in serum was significantly higher in the HPA group compared to the CPA group (p < 0.05). Levels of glucose, proteins, albumin, and hepatorenal biomarkers were higher in active groups compared with the sedentary group. It was demonstrated that the increased activities of LDH (mainly caused by higher activity of isoform LDH2) and mMDH were found under the condition of homocysteine-treated rats plus aerobic physical activity. Independent application of homocysteine did not lead to these changes. Physical activity leads to activation of MMP-2 isoform and to increased activity of MMP-9 isoform in both homocysteine-treated and control rats.

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Regulation of malate dehydrogenase activity by glutamate, citrate, alpha-ketoglutarate, and multienzyme interaction.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)38026-8 ◽

1988 ◽

Vol 263 (22) ◽

pp. 10687-10697 ◽

Cited By ~ 5

Author(s):

L A Fahien ◽

E H Kmiotek ◽

M J MacDonald ◽

B Fibich ◽

M Mandic

Keyword(s):

Dehydrogenase Activity ◽

Malate Dehydrogenase

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