Four Weeks of Aerobic Training Affects Cardiac Tissue Matrix Metalloproteinase, Lactate Dehydrogenase and Malate Dehydrogenase Enzymes Activities, and Hepatorenal Biomarkers in Experimental Hyperhomocysteinemia in Rats

The aim of this study was to investigate the effect of the application of homocysteine as well as its effect under the condition of aerobic physical activity on the activities of matrix metalloproteinases (MMP), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in cardiac tissue and on hepato-renal biochemical parameters in sera of rats. Male Wistar albino rats were divided into four groups (n = 10, per group): C: 0.9% NaCl 0.2 mL/day subcutaneous injection (s.c.); H: homocysteine 0.45 µmol/g b.w./day s.c.; CPA saline (0.9% NaCl 0.2 mL/day s.c.) and a program of physical activity on a treadmill; and HPA homocysteine (0.45 µmol/g b.w./day s.c.) and a program of physical activity on a treadmill. Subcutaneous injection of substances was applied 2 times a day at intervals of 8 h during the first two weeks of experimental protocol. Hcy level in serum was significantly higher in the HPA group compared to the CPA group (p < 0.05). Levels of glucose, proteins, albumin, and hepatorenal biomarkers were higher in active groups compared with the sedentary group. It was demonstrated that the increased activities of LDH (mainly caused by higher activity of isoform LDH2) and mMDH were found under the condition of homocysteine-treated rats plus aerobic physical activity. Independent application of homocysteine did not lead to these changes. Physical activity leads to activation of MMP-2 isoform and to increased activity of MMP-9 isoform in both homocysteine-treated and control rats.

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Sucralose induced pancreatic toxicity in albino rats: Histomorphological evidence

Journal of Morphological Sciences ◽

10.4322/jms.073614 ◽

2014 ◽

Vol 31 (02) ◽

pp. 123-127 ◽

Cited By ~ 1

Author(s):

S. Gupta ◽

S. Kalra ◽

V. Bharihoke ◽

D. Dhurandhar

Keyword(s):

Lethal Dose ◽

Nutrient Sensing ◽

Albino Rats ◽

Artificial Sweetener ◽

Distilled Water ◽

Obesity And Diabetes ◽

Pancreatic Toxicity ◽

Treatment Of Obesity ◽

Wistar Albino Rats ◽

And Control

AbstractIn the present world people have become conscious of the fact that sugar is one of the commonest culprits for gain in weight and blood sugar in diabetes. One of the artificial sweetener, sucralose is widely used by diabetics is still under strict scrutiny because of the contradictory feedbacks obtained from various studies, often discouraging its use. Therefore, there arises a need to check whether the use of sucralose is safe or not. The present study was designed to determine to evaluate and compare the histological changes of sucralose on the islets of pancreas in albino rats. Methods: The adult Wistar albino rats were given sucralose orally by gavage in the dose of 3gms/kg body weight/day dissolved in distilled water and only distilled water for 30 days to experimental and control groups respectively. The animals were weighed prior to and after the experiment. The animals were sacrificed on day 31. The pancreas was dissected and observed grossly. Tissue was processed; paraffin blocks were prepared and 8 micron thick sections were cut. Sections were stained with Haemotoxylin and Eosin and Gomori's stains. Results: Pancreatic toxicity was observed in the form of vaculation of islets, lymphocytic infilterate, degeneration of islets and acini of Pancreas. The commonly used non-lethal dose of sucralose induced damage to Pancreas is startling. Conclusion: The results obtained have wide implications for nutrient sensing and nutrition in the treatment of obesity and diabetes.

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Investigation into the Possible Risk Effects Associated with Drug and Alcohol-induced Gastrointestinal Ulcer in Wistar Albino Rats

Annual Research & Review in Biology ◽

10.9734/arrb/2019/v32i330085 ◽

2019 ◽

pp. 1-8

Author(s):

Oladayo E. Apalowo ◽

Oladayo J. Areola ◽

Gbenga S. Ogunleye ◽

Ayodeji S. Odukoya ◽

Olusegun O. Babalola

Keyword(s):

Creatine Kinase ◽

Gastric Ulcer ◽

Lactate Dehydrogenase ◽

Kinase Activity ◽

Creatine Kinase Activity ◽

Albino Rats ◽

Ulcer Disease ◽

Gastrointestinal Ulcer ◽

Wistar Albino Rats ◽

Three Body

Aim: The study investigated the possible risks associated with gastrointestinal ulcer disease by evaluating the biochemical response of three body organs; heart, kidney and liver, in gastric ulcerated rats. Methodology: Twenty male wistar albino rats were used in the study. Gastric ulcer was induced in rats with single oral dose of 400 mg/kg body weight (b.w.) aspirin, 80 mg/kg b.w. indomethacin and 5 ml/kg b.w. acidified ethanol (40:60 v/v). Blood samples were collected into heparinized bottle and centrifuged at 4000 rpm for 10 mins to obtain the plasma. Gastric tissue, liver, kidney and heart were also collected. Results: Oral administration of 400 mg/kg b.w. aspirin, 80 mg/kg b.w. indomethacin and 5 ml/kg b.w. acidified ethanol caused a remarkable increase in ulcer index. There was observed a significant (p<0.05) reduction in AST and ALT activities in gastric ulceration caused by aspirin (Asp), with no significant (p<0.05) change in total protein (TP) concentration, lactate dehydrogenase and creatine kinase activity. However, there was increase in creatinine and urea concentration. Acidified ethanol and Indomethacin-induced ulcerated rats showed significant (p<0.05) reduction in all other parameters except ALT and lactate dehydrogenase activities which did not show any significant (p<0.05) change. There was also observed a significant (p<0.05) increase in creatine kinase activity in indomethacin-induced ulcerated rats. Conclusion: Overall, the result indicates a link between gastric ulcer and organ toxicity. The use of NSAIDs above the therapeutic doses in the treatment of pains and related illness as well as excess consumption of alcohol is shown to negatively impact the stomach and cause serious damage to different body organs of wistar rats.

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The protective and therapeutic effects of linalool against doxorubicin-induced cardiotoxicity in Wistar albino rats

Human & Experimental Toxicology ◽

10.1177/0960327119842634 ◽

2019 ◽

Vol 38 (7) ◽

pp. 803-813 ◽

Cited By ~ 4

Author(s):

Z Oner ◽

E Altınoz ◽

H Elbe ◽

N Ekinci

Keyword(s):

Caspase 3 ◽

Inflammatory Cell ◽

Cardiac Tissue ◽

Saline Solution ◽

Albino Rats ◽

Therapeutic Effects ◽

Histopathological Changes ◽

Treatment Groups ◽

Wistar Albino Rats ◽

Plasma Creatine Kinase

The aim of the present study was to determine the protective and therapeutic effects of linalool (LIN) against doxorubicin (DOX)-induced cardiotoxicity in rats histologically and biochemically. In experiments, 64 male Wistar albino rats were randomly divided into eight groups ( n = 8). These groups were control (C) (0.9% saline solution), DOX (20 mg/kg DOX), LIN50 (50 mg/kg LIN), LIN100 (100 mg/kg LIN), DOX + LIN50 (20 mg/kg DOX and 50 mg/kg LIN), DOX + LIN100 (20 mg/kg DOX and 100 mg/kg LIN), LIN50 + DOX (50 mg/kg LIN and 20 mg/kg DOX), and LIN100 + DOX (100 mg/kg LIN and 20 mg/kg DOX). It was determined that necrosis and extensive inflammatory cell infiltration were observed in the DOX group. It was determined that histopathological changes significantly decreased in groups treated with LIN after DOX administration. While the caspase-3 immunostaining was highly evident in DOX group apoptotic cells ( p < 0.001, for all), the intensity of caspase-3 immunostaining in the treatment groups decreased ( p < 0.05). While DOX administration resulted in a significant increase in malondialdehyde (MDA) levels and plasma Creatine kinase (CK) and lactate dehydrogenase (LDH) levels in cardiac tissue when compared to the C groups, it was observed that DOX + LIN administration led to a significant decrease in MDA, plasma CK and LDH levels and a significant increase in glutathione (GSH), superoxide dismutase, and catalase enzyme levels. Finally, it was concluded that DOX led to heavy cardiotoxicity and DOX + LIN administration could remove cardiomyopathy symptoms.

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The influence of subchronic co-application of vitamins B6 and folic acid on cardiac oxidative stress and biochemical markers in monocrotaline-induced heart failure in male Wistar albino rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/cjpp-2019-0305 ◽

2020 ◽

Vol 98 (2) ◽

pp. 93-102 ◽

Cited By ~ 1

Author(s):

Jovana Jakovljevic Uzelac ◽

Tatjana Djukic ◽

Slavica Mutavdzin ◽

Sanja Stankovic ◽

Milica Labudovic Borovic ◽

...

Keyword(s):

Oxidative Stress ◽

Heart Failure ◽

Folic Acid ◽

Vitamin B6 ◽

Cardiac Tissue ◽

Troponin T ◽

Physiological Saline ◽

Albino Rats ◽

Wistar Albino Rats ◽

The Right

The aim of this study was to test the hypothesis that subchronic co-application of vitamins B6 and folic acid (FA) could affect heart failure (HF) induced by monocrotaline (MCT), with the modulation of oxidative stress parameters and cardiometabolic biomarkers. Biochemical and histomorphometric analyses were assessed in blank solution-exposed controls (C1 physiological saline 1 mL/kg, 1 day, n = 8; C2 physiological saline 1 mL/kg, 28 days, n = 8), MCT-induced HF (MCT 50 mg/kg, n = 8), B6+FA (vitamin B6 7 mg·kg–1·day–1, FA 5 mg·kg–1·day–1; n = 8), and MCT+B6+FA (MCT 50 mg/kg, vitamin B6 7 mg·kg–1·day–1, FA 5 mg·kg–1·day–1; n = 8) in male Wistar albino rats (body mass 160 g at the start). Superoxide dismutase and glutathione peroxidase activities, thiol-, carbonyl groups, and nitrotyrosine were determined in cardiac tissue. Echocardiography was performed to confirm MCT-induced HF. The right ventricular wall hypertrophy, accompanied with significant increase of troponin T and preserved renal and liver function, has been shown in MCT-induced HF. However, these effects were not related to antioxidant effects of vitamin B6 and FA, since several parameters of oxidative stress were more pronounced after treatment. In this study, co-application of vitamins B6 and FA did not attenuate hypertrophy of the right ventricle wall but aggravated oxidative stress, which is involved in HF pathogenesis.

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Copper-induced changes of lipid peroxidation and hemato-biochemical parameters in rat blood: Protective role of flavonoids

Archives of Biological Sciences ◽

10.2298/abs1403271m ◽

2014 ◽

Vol 66 (3) ◽

pp. 1271-1279 ◽

Cited By ~ 3

Author(s):

Jelena Mladenovic ◽

Milica Paunovic ◽

Milos Matic ◽

Veroljub Knezevic ◽

Branka Ognjanovic ◽

...

Keyword(s):

Lipid Peroxidation ◽

Lactate Dehydrogenase ◽

Biochemical Parameters ◽

Lipid Peroxides ◽

Protective Role ◽

Albino Rats ◽

Biochemical Processes ◽

Wistar Albino Rats ◽

Induced Changes

The effects of subchronic exposure to copper (Cu) on lipid peroxidation, hemato-biochemical parameters, and the possible protective role of flavonoids Quercetin and (-)-Epicatechin were studied. Male Wistar albino rats were treated with Cu (560 mg/L, p.o. as CuCl2?2H2O for 5 weeks) and Quercetin and (-)-Epicatechin (40 mg/kg BW each, i.p., every third day during the last 3 weeks) alone or in combination. Cu increased the concentration of lipid peroxides, decreased the number of erythrocytes, hemoglobin and hematocrit values and increased the activities of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase. Coadministration of Quercetin and (-)-Epicatechin with Cu lowered the process of lipid peroxidation and restored examined hemato-biochemical parameters to control values. Our results indicate that Cu induced oxidative damage in erythrocytes, which led to anemia, while Quercetin and (-)-Epicatechin showed a protective effect on the hemato-biochemical processes in the blood of rats.

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In Vivo Evaluation of Nano-HA/PDLLA Composite

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.284-286.881 ◽

2005 ◽

Vol 284-286 ◽

pp. 881-884 ◽

Cited By ~ 1

Author(s):

Yu Mei Xiao ◽

Hong Song Fan ◽

Yao Wu ◽

Jin Rui Xu ◽

Y. Tan ◽

...

Keyword(s):

Histological Analysis ◽

Sem Analysis ◽

Albino Rats ◽

Degradation Behavior ◽

Porous Network ◽

In Vivo Evaluation ◽

Good Biocompatibility ◽

Wistar Albino Rats ◽

And Control

The purpose of this study was to evaluate the behavior of nano-hydroxyapatite/ poly(D,L)lactide (n-HA/PDLLA) composite in vivo. The composite rods containing about 40wt% n-HA and control HA rods with a diameter of 2mm and a length of 6mm were implanted into the femora of 16 New Zealand rabbits. Composite wafers with a diameter of 5mm and a thickness of 1mm were implanted into the dorsal subcutis of 18 Wistar Albino rats. After definite intervals, the histological analysis was completed by light microscopy and the degradation behavior was observed by scanning electron microscopy. The histological analysis showed no obvious difference between n-HA /PDLLA composite and pure HA that had good biocompatibility and osteoconductivity. SEM analysis of the surface and cross section of the samples showed that the degradation of the composite started from surface, then into the inner gradually and formed multiple pores at surface. The pore size and porosity gradually increased along with time and a porous network may be formed.

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Antioxidant properties of Rosa pisiformis and its protective effect against isoproterenol-induced oxidative stress in rats / Rosa pisiformis’in antioksidant özellikleri ve izoproterenol ile ratlarda oluşturulan okdidatif strese karşı koruyucu etkisi

Turkish Journal of Biochemistry ◽

10.1515/tjb-2016-0034 ◽

2016 ◽

Vol 41 (4) ◽

Author(s):

Mahire Bayramoğlu ◽

Suat Ekin ◽

Hatice Kızıltaş ◽

Gökhan Oto ◽

Ebru Altındal Susen ◽

...

Keyword(s):

Oxidative Stress ◽

Cardiac Tissue ◽

Histopathological Examination ◽

Antioxidant Properties ◽

Albino Rats ◽

Cardiac Tissues ◽

Positive Effects ◽

Icp Ms ◽

Wistar Albino Rats ◽

Method R

AbstractObjective: In this study, Rosa pisiformis was evaluated for its antioxidant activity, vitamin (A, E, C), trace element (Fe, Cu, Zn, Mn, Cr, Se) and mineral (Ca, K, Mg, Na) levels, in addition to its cardioprotective effects on cardiac tissue antioxidant enzymes (GSH-Px, SOD, CAT) during isoproterenol (ISO) - induced oxidative stress.Methods: In the study, vitamin and mineral analysis was carried out using HPLC and ICP-MS method, R. P. antioxidant and antiradical properties were determined using spectrophotometer. Forty wistar albino rats were divided into four groups: control (0.9% NaCl), ISO (100 mg/kg), ISO (100 mg/kg) + R. P. (300 mg/kg) and R. P. (300 mg/ kg). ISO was injected subcutaneously into rats twice at an interval of 24 h for two consecutive days (on 28Results: The results of the study indicated that R. P. fruit and leaves contain high levels of minerals and vitamins and possess antioxidant and antiradical activity and its fruits have positive effects on cardiac tissues. The results are supported with significant changes in antioxidant enzyme levels and histopathological examination.Conclusion: This study demonstrates that R. P. fruits in particular can be used to treat and prevent cardiac diseases.

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Stereological examination of effects of ethanol on optic nerve in experimental alcohol model

Human & Experimental Toxicology ◽

10.1177/0960327119828123 ◽

2019 ◽

Vol 38 (5) ◽

pp. 610-615 ◽

Cited By ~ 1

Author(s):

T Igit ◽

N Colcimen

Keyword(s):

Optic Nerve ◽

Control Group ◽

Albino Rats ◽

Optic Chiasma ◽

Ethanol Group ◽

Time Period ◽

Examination Methods ◽

Wistar Albino Rats ◽

Induced Changes ◽

And Control

The objective of the present study was to perform histological and stereological examination of alcohol-induced changes in the optic nerve, considered an extension of white matter of the brain, in rats. This study included 20 male Wistar albino rats aged 60 days and weighing 190–220 g. The rats were divided into three groups: ethanol ( n = 7), maltodextrin ( n = 7), and control ( n = 6) groups. The ethanol group was administered ethanol at a dose of 6.4% (v/v) instead of water for 18 days; the maltodextrin group received maltodextrin for the same time period, and the control group was the sham group. At the end of the experiment, a 0.5-mm long section of the optic nerve starting from the optic chiasma was dissected and examined with routine microscopic histological examination methods. The modified Cavalieri method was used for stereological measurement. Total tissue area ratios were calculated with a point grid provided by the Shtereom 1.5 software package. The statistical comparison of the groups revealed that the ethanol group had a significant reduction in the number of axons and sheath area of the optic nerve compared to the control and maltodextrin groups ( p < 0.017, p < 0.022, respectively). These results indicate the toxic effects of ethanol on the optic nerve.

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Effect of Ethanol Extract of Cucurbita pepo Leaves on the Lipid Profile of Wistar Albino Rats

Asian Journal of Research in Biochemistry ◽

10.9734/ajrb/2018/v2i4601 ◽

2018 ◽

pp. 1-7

Author(s):

F. U. Eneh ◽

G. C. Ugochukwu ◽

C. M. Okoye

Keyword(s):

Lipid Profile ◽

Test Group ◽

Ethanol Extract ◽

Albino Rats ◽

Phytochemical Investigation ◽

Significant Difference ◽

The Rich ◽

Wistar Albino Rats ◽

And Control

Aim: The role of nutrition in lipid metabolism continues to generate a lot of research interest, especially as dyslipidaemia is implicated in a host of diseases. The work investigated the effect of ethanol extract of Curcubita pepo leaves on the lipid profile of Wister rats. Methodology: A total of 18 rats divided into three groups of 6 rats each were employed in the investigation. The first group (baseline) was sacrificed after purchase; second group (control) was fed rat chow, and the third group (test) was fed a composite feed containing rat chow and 10% extract of C. pepo leaves. Results: The lipid profile (total cholesterol, triglycerides, LDL, HDL and VLDL) of the rats was assayed after 18 days of feeding and the result showed no significant difference (P>0.05) between the test group and control for the lipids assayed. However, there was a marked increase in the HDL level of the test group (0.500±0.057 mmol/L) compared to the control (0.268±0.043 mmol/L), it was nonetheless statistically non-significant (P=0.068). The HDL increasing effect observed may stem from the rich phytochemistry of the leaves, as preliminary phytochemical investigation showed the leaves to contain saponin, tannin, alkaloids, flavonoids and glycosides. Conclusion: We conclude that the leaves of C. pepo may play a medicinal role in maintaining lipid homeostasis.

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EXPERIMENTAL EVALUATION OF TRISHNAHARA (THIRST RELIEVING) PROPERTY OF TAKRABHEDA (BUTTERMILK) MENTIONED IN BHAVAPRAKASHA IN GREESHMARITU

AYUSHDHARA ◽

10.47070/ayushdhara.v7i2.536 ◽

2020 ◽

pp. 2631-2635

Author(s):

Amritha MS ◽

Santhosh C ◽

N.Manojkumar

Keyword(s):

Statistical Analysis ◽

Literature Review ◽

Summer Season ◽

The Body ◽

Control Group ◽

Albino Rats ◽

Indian Culture ◽

Wistar Albino Rats ◽

Post Hoc ◽

And Control

Takra is one of the important Paniya (drinks) in Indian culture. It is common drink used abundantly especially during the summer season. The main reason for its ample usage as a drink during summer might be the sudden relief it provides to the thirst and fatigue due to extreme heat. Literature review on the Ayurvedic concept of Takra shows it possesses Ushna Veerya and Amla Vipaka, which in turn increases the Pitta inside the body and will leads to thirst rather than decreasing thirst especially in Greehma Ritu. Thus, there exist a paradox in the literature and practice. Thus a study was conducted to find out the Trishnahara property of Takrabheda in Greeshma Ritu. In the experiment total intake of water by each group after administering specific variety of buttermilk were calculated. The study was conducted during Greeshmaritu in wistar albino rats. Numbers of groups were six which included Ghola, Matita, Takra, Udasvit Caccika and Control. statistical analysis was done by ANOVA followed by Dunnet multiple comparison as post hoc test, if p<0.05 using graph pad instant software and by ANOVA followed by Tukey Kramer as post hoc test, if p<0.05 using graph pad instant software. It is concluded that Takrabheda is not having Trishnahara property when compared with the control group in Greeshma Ritu because of its Ushna, Ruksha Guna of Takrabheda. But in Vasanta Ritu, Acarya advised the intake of Dipana, Laghu and Ruksha Ahara. So, Takra can be an effective Paniya during Vasanta Rithu.

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