scholarly journals Adsorption of anti-C-Reactive Protein Monoclonal Antibody and Its F(ab')2 fragment on Plasma-Polymerized Styrene, Allylamine and Acrylic Acid Coated with Quartz Crystal Microbalance.

2002 ◽  
Vol 15 (2) ◽  
pp. 323-329 ◽  
Author(s):  
Shigeru Kurosawa ◽  
Tomoya Hirokawa ◽  
Kazuya Kashima ◽  
Hidenobu Aizawa ◽  
Jong-Won Park ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Acrylic Acid ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Ning Gan ◽  
Ping Xiong ◽  
Ji Wang ◽  
Tianhua Li ◽  
Futao Hu ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Microtiter Plate ◽  
Electrochemical Quartz Crystal Microbalance ◽  
Magnetic Core ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Insoluble Product

A novel horseradish peroxidase- (HPR-) doped magnetic core-shell Fe3O4@SiO2@Au nanocomposites (Fe-Au MNPs) were employed on immunoassay for the determination of C-reactive protein (CRP) based on a electrochemical quartz crystal microbalance detector (EQCM). Firstly, the secondary CRP antibody and HRP were both immobilized on the Fe-Au MNPs (Fe-Au MNPs-anti-CRP2/HRP) as a signal tag. Secondly, the above tag and the primary antibody (anti-CRP1) in the bottom of 96-well microtiter plate were employed to conjugate with a serial of CRP concentrations to produce a sandwich immunocomplex. Thirdly, the immunocomplex solution was subsequently exposed to3,3′-diaminobenzidine (DAB) in the presence of H2O2, resulting in an insoluble product. When the precipitation solution was dripped on EQCM, it can achieve a decrease of frequency of crystal (Δf). The amount ofΔfwas proportional to (CRP) from 0.003 to 200 ng mL−1with a low detection limit of 1 pg mL−1. Compared with the enzyme-linked immunosorbent assay (ELISA), the immunoassay shows greatly improved sensitivity due to the significant amount of HRP labeled on signal tag. It also has good specificity and low sample consumption, which is expected to be a benefit for the CRP screening in early diagnosis of cardiovascular disease.

scholarly journals Detection of C-Reactive Protein in Serum Using Resonant Property of Quartz Crystal Microbalance

2006 ◽  
Vol 74 (2) ◽  
pp. 156-158 ◽  
Author(s):  
Hidenobu AIZAWA ◽  
Shigeru KUROSAWA ◽  
Mitsuhiro TOZUKA ◽  
Chikako KUROSAWA ◽  
Jong-Won PARK ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Resonant Property

scholarly journals Recombinant antibody fragments allow repeated measurements of C-reactive protein with a quartz crystal microbalance immunosensor

mAbs ◽  
2013 ◽  
Vol 5 (1) ◽  
pp. 140-149 ◽  
Author(s):  
Laila Al-Halabi ◽  
Anne Balck ◽  
Monika Michalzik ◽  
David Fröde ◽  
Stephanus Büttgenbach ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Repeated Measurements ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Recombinant Antibody Fragments

scholarly journals Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 159
Author(s):  
Satit Rodphukdeekul ◽  
Miyuki Tabata ◽  
Chindanai Ratanaporncharoen ◽  
Yasuo Takeuchi ◽  
Pakpum Somboon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quantitative Assessment ◽  
Quartz Crystal ◽  
Dynamic Range ◽  
Gold Surface ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Disorder ◽  
Label Free ◽  
Periodontal Bacteria

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.

Sign in / Sign up

Export Citation Format

Share Document