Development of indirect-competitive quartz crystal microbalance immunosensor for C-reactive protein

2009 ◽  
Vol 143 (1) ◽  
pp. 444-448 ◽  
Author(s):  
Namsoo Kim ◽  
Dong-Kyung Kim ◽  
Yong-Jin Cho
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
C Reactive Protein ◽  
Reactive Protein

scholarly journals A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Ning Gan ◽  
Ping Xiong ◽  
Ji Wang ◽  
Tianhua Li ◽  
Futao Hu ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Microtiter Plate ◽  
Electrochemical Quartz Crystal Microbalance ◽  
Magnetic Core ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Insoluble Product

A novel horseradish peroxidase- (HPR-) doped magnetic core-shell Fe3O4@SiO2@Au nanocomposites (Fe-Au MNPs) were employed on immunoassay for the determination of C-reactive protein (CRP) based on a electrochemical quartz crystal microbalance detector (EQCM). Firstly, the secondary CRP antibody and HRP were both immobilized on the Fe-Au MNPs (Fe-Au MNPs-anti-CRP2/HRP) as a signal tag. Secondly, the above tag and the primary antibody (anti-CRP1) in the bottom of 96-well microtiter plate were employed to conjugate with a serial of CRP concentrations to produce a sandwich immunocomplex. Thirdly, the immunocomplex solution was subsequently exposed to3,3′-diaminobenzidine (DAB) in the presence of H2O2, resulting in an insoluble product. When the precipitation solution was dripped on EQCM, it can achieve a decrease of frequency of crystal (Δf). The amount ofΔfwas proportional to (CRP) from 0.003 to 200 ng mL−1with a low detection limit of 1 pg mL−1. Compared with the enzyme-linked immunosorbent assay (ELISA), the immunoassay shows greatly improved sensitivity due to the significant amount of HRP labeled on signal tag. It also has good specificity and low sample consumption, which is expected to be a benefit for the CRP screening in early diagnosis of cardiovascular disease.

scholarly journals Detection of C-Reactive Protein in Serum Using Resonant Property of Quartz Crystal Microbalance

2006 ◽  
Vol 74 (2) ◽  
pp. 156-158 ◽  
Author(s):  
Hidenobu AIZAWA ◽  
Shigeru KUROSAWA ◽  
Mitsuhiro TOZUKA ◽  
Chikako KUROSAWA ◽  
Jong-Won PARK ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Resonant Property

scholarly journals Recombinant antibody fragments allow repeated measurements of C-reactive protein with a quartz crystal microbalance immunosensor

mAbs ◽  
2013 ◽  
Vol 5 (1) ◽  
pp. 140-149 ◽  
Author(s):  
Laila Al-Halabi ◽  
Anne Balck ◽  
Monika Michalzik ◽  
David Fröde ◽  
Stephanus Büttgenbach ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Repeated Measurements ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Recombinant Antibody Fragments
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