Seed Development in Self-pollination of 4X Hyuganatsu and Reciprocal Crosses between 2X and 4X Hyuganatsu, and Overcoming the Self-incompatibility of 2X Hyuganatsu Using Pollen of 4X Hyuganatsu

Dendrobium officinale is a rare and traditional medicinal plant with high pharmacological and nutritional value. The self-incompatibility mechanism of D. officinale reproductive isolation was formed in the long-term evolution process, but intraspecific hybridization of different germplasm resources leads to a large gap in the yield, quality, and medicinal value of D. officinale. To investigate the biological mechanism of self-incompatibility in D. officinale, cytological observation and the transcriptome analysis was carried out on the samples of self-pollination and cross-pollination in D. officinale. Results for self-pollination showed that the pollen tubes could grow in the style at 2 h, but most of pollen tubes stopped growing at 4 h, while a large number of cross-pollinated pollen tubes grew along the placental space to the base of ovary, indicating that the self-incompatibility of D. officinale may be gametophyte self-incompatibility. A total of 63.41 G basesum of D. officinale style samples from non-pollinated, self-pollination, and cross-pollination by RNA-seq were obtained, and a total of 1944, 1758, and 475 differentially expressed genes (DEGs) in the comparison of CK (non-pollinated) vs. HF (cross-pollination sample), CK vs. SF (self-pollination sample) and SF vs. HF were identified, respectively. Forty-one candidate genes related to self-incompatibility were found by function annotation of DEGs, including 6 Ca2+ signal genes, 4 armed repeat containing (ARC) related genes, 11 S-locus receptor kinase (SRK) related genes, 2 Exo70 family genes, 9 ubiquitin related genes, 1 fatty acid related gene, 6 amino acid-related genes, 1 pollen-specific leucine-rich repeat extensin-like protein (LRX) related gene and 1 lectin receptor-like kinases (RLKs) related gene, showed that self-incompatibility mechanism of D. officinale involves the interaction of multiple genes and pathways. The results can provide a basis for the study of the self-incompatibility mechanism of D. officinale, and provide ideas for the preservation and utilization of high-quality resources of D. officinale.

Download Full-text

Late-acting self-incompatibility in Acca sellowiana (Myrtaceae)

Australian Journal of Botany ◽

10.1071/bt10152 ◽

2011 ◽

Vol 59 (1) ◽

pp. 53 ◽

Cited By ~ 12

Author(s):

Taciane Finatto ◽

Karine L. Dos Santos ◽

Neusa Steiner ◽

Leon Bizzocchi ◽

Daniel F. Holderbaum ◽

...

Keyword(s):

Fruit Development ◽

The Self ◽

The Other ◽

Zygote Formation ◽

Self Incompatibility ◽

Self Pollination ◽

Significant Difference ◽

Natural Pollination ◽

Acca Sellowiana ◽

Allogamous Species

Acca sellowiana (Berg) Burret is a predominantly allogamous species with hermaphrodite flowers that has barriers to self-fertilisation such as dichogamy by protogyny and self-incompatibility. This study aimed to identify when self-incompatibility occurs in A. sellowiana flowers submitted to self-pollinations. Pollinations were made using nine known self-incompatible accessions in several treatments including manual cross-pollination, manual self-pollination, no pollination and natural pollination. Flowers were pollinated and pistils collected at times ranging from 1 to 30 days after pollination (DAP). In both cross- and self-pollinations the fertilisation occurred from 18 DAP and the zygote was visualised at 24 and 26 DAP, respectively. The abscission of the self-pollinated flowers increased from 26 DAP onwards, when significant differences (P < 0.05) were found for the percentage of abscission among self-pollinated and cross-pollinated flowers. At 30 DAP, 72% of self-pollinated flowers were abscised. The size of ovules and ovaries of self-pollinated flowers showed no significant difference from those of non-pollinated flowers throughout 30 DAP, while those from cross-pollinated flowers were significantly larger (P < 0.05) than the other treatments from 22 and 24 DAP, respectively. After 40 DAP, there was no fruit development in self-pollination and non-pollination treatments. Our study brings greater clarity to the mechanism of self-incompatibility in A. sellowiana, indicating late-acting self-incompatibility occurring through the rejection/abscission of self-pollinated flowers precisely after syngamy and zygote formation.

Download Full-text

Late-acting self-incompatibility in tea plant (Camellia sinensis)

Biologia ◽

10.2478/s11756-012-0018-9 ◽

2012 ◽

Vol 67 (2) ◽

Cited By ~ 11

Author(s):

Xuan Chen ◽

Shan Hao ◽

Li Wang ◽

Wanping Fang ◽

Yuhua Wang ◽

...

Keyword(s):

Pollen Tube ◽

Camellia Sinensis ◽

Pollen Tubes ◽

Tea Plant ◽

The Self ◽

Self Incompatibility ◽

Cross Pollination ◽

Self Pollination ◽

Hand Pollination ◽

Pollen Tube Elongation

AbstractThe self-incompatibility of tea plant (Camellia sinensis (L.) O. Kuntze) was studied with the methods of aniline blue fluorescence assay and paraffin sections. The characteristics of pollen tube elongation after hand pollination was analyzed in 4 tea cultivars, including ‘Keemenzhong’, ‘Longjing-changye’, ‘Fuding-dabaicha’ and ‘Yabukita’, under self-pollination and cross-pollination, respectively. Although there were some difference among cultivars, pollen tubes elongated through the style and reach the ovary successfully at 48 h after pollination for both cross- and self-pollen tubes in all the four cultivars of tea. Pollen tubes entered into the ovule micropyles, however, only for cross-pollination, but not for self-pollination. Pollen tubes of selfing plants, failed in fertilizing, seemed have some difficulties to enter the ovule. All of which indicated that the self-incompatibility of tea plant is a late-acting self-incompatibility system (LSI) or an ovarian sterility (OS), in which the self incompatibility was due to none self pollen tube penetrating into the ovule and no fertilization.

Download Full-text

Carbon Dioxide Treatment Partially Overcomes Self-incompatibility in a Cacao Genotype

HortScience ◽

10.21273/hortsci.29.1.15 ◽

1994 ◽

Vol 29 (1) ◽

pp. 15-17 ◽

Cited By ~ 8

Author(s):

Madhu Aneja ◽

Thomas Gianfagna ◽

Edward Ng ◽

Ignacio Badilla

Keyword(s):

Theobroma Cacao ◽

Pollen Germination ◽

Fruit Set ◽

The Self ◽

High Rate ◽

Self Incompatibility ◽

Self Pollination ◽

Promotive Effect ◽

Two Stages ◽

Germination Stage

Cacao (Theobroma cacao L.) contains self-compatible and self-incompatible genotypes. In the greenhouse, pollen germination and fruit set failed to occur after self-pollination of an incompatible genotype (IMC 30); however, if the self-pollinated flowers were enclosed in plastic vials for 6 h after pollination, pollen germination was 95% The promotive effect of enclosed pollination on pollen germination was due to the accumulation of CO2 (8.9 % v/v). Despite the high rate of pollen germination, fruit set was only 45%. Seeds produced from self-pollinations using this technique were viable, with 95% germination. Cross-pollination with `Amelonado' pollen resulted in 100% pollen germination and 46% fruit set. Enclosure of cross-pollinated flowers did not improve the percentage of fruit set. Sections made through the ovary 48 h after enclosed self-pollination indicated that the majority of ovules contained a zygote; however, some ovules still contained unfused male and female gametes and polar nuclei. Self-incompatibility in this genotype is expressed at two stages in the process leading to fruit set. The first is at the pollen germination stage and can be overcome by CO2 treatment; the second is at the gametic fusion stage.

Download Full-text

METHOD FOR OVERCOMING THE PASSION FRUIT SELF-INCOMPATIBILITY

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452016477 ◽

2016 ◽

Vol 38 (4) ◽

Author(s):

JOSÉ SEVERINO DE LIRA JÚNIOR ◽

CLÁUDIO HORST BRUCKNER ◽

PEDRO CRESCÊNCIO SOUZA CARNEIRO

Keyword(s):

Fruit Set ◽

The Self ◽

Passion Fruit ◽

Self Incompatibility ◽

Seed Number ◽

Flower Bud ◽

Inbred Population ◽

Self Pollination ◽

Fruit Mass

ABSTRACT The methodology of self-pollination of passion fruit flowers at anthesis after stigma excision was proposed. This method was compared with self-pollination procedures at anthesis and at the stage of flower bud. Two genotypes (M7 and N9), propagated by cuttings were studied. Self-pollinations were performed at 1 pm, 1 pm and 5 pm or 5 pm. Fruit set (%), fruit mass (g), pulp mass (g), pulp/fruit ratio and seed number per fruit were evaluated and compared to fruits derived from M7 x N9 and N9 x M7 crosses. No fruit set was obtained from self-pollination at anthesis, which confirms the self-incompatibility of both genotypes. Self-pollination after stigma excision was the best procedure, reaching average fruit set of 73.61 % (13 hours) and 81.94 % (double self-pollination at 1 pm and 5 pm). Although two self-pollinations after stigma excision at anthesis is more efficient, performing only one self-pollination at 1 pm requires less labor. The amount of seeds produced per fruit is satisfactory to generate inbred population.

Download Full-text

Reproductive Characteristics for Self-compatibility and Seedlessness in ‘Nishiuchi Konatsu’, a Bud Mutation of Hyuganatsu (Citrus tamurana hort. ex Tanaka)

HortScience ◽

10.21273/hortsci.44.6.1547 ◽

2009 ◽

Vol 44 (6) ◽

pp. 1547-1551 ◽

Cited By ~ 5

Author(s):

Chitose Honsho ◽

Masami Kotsubo ◽

Yuri Fukuda ◽

Yosui Hamabata ◽

Yoshikazu Kurogi ◽

...

Keyword(s):

Genetic Resources ◽

The Self ◽

Seed Abortion ◽

Self Incompatibility ◽

Fruit Characteristics ◽

Reproductive Characteristics ◽

Bud Mutation ◽

Self Pollination ◽

Self Compatibility

The reproductive characteristics of ‘Nishiuchi Konatsu’, a bud mutation of Hyuganatsu that is self-incompatible, were examined. A pollination experiment resulted in Hyuganatsu × ‘Nishiuchi Konatsu’ and ‘Nishiuchi Konatsu’ self-pollination produced their fruit, whereas ‘Nishiuchi Konatsu’ × Hyuganatsu cannot produce fruit as a result of early dropping of premature fruits within the first 10 weeks after pollination. Considering the self-incompatible nature of Hyuganatsu, this result implies a mutation of the pollen-expressed gene(s) associated with the self-incompatibility occurring in ‘Nishiuchi Konatsu’. When ‘Nishiuchi Konatsu’ pollen was used for several citrus accessions (Hyuganatsu, ‘Nishiuchi Konatsu’, Hassaku, and ‘Sweet Spring’), it was observed that the number of normal seeds decreased and that of aborted seeds increased. Interestingly, the extent of seed abortion differed among seed parents. Hyuganatsu and ‘Nishiuchi Konatsu’ lost most of their normal seeds. In contrast, Hassaku lost relatively lesser seeds, and ‘Sweet Spring’ lost only a few seeds. Moreover, Hassaku fruits obtained by pollination with ‘Nishiuchi Konatsu’ pollen showed various proportions of normal and aborted seeds even among individual fruits. ‘Nishiuchi Konatsu’ pollen did not affect the fruit characteristics, with some exceptions in Hyuganatsu, ‘Nishiuchi Konatsu’, and Hassaku. All the characteristics were superior in Hyuganatsu-pollinated fruits compared with ‘Nishiuchi Konatsu’-pollinated ones in ‘Sweet Spring’. In conclusion, it was demonstrated that ‘Nishiuchi Konatsu’ has two agriculturally important traits: self-compatibility and seed abortion. It can be useful for elucidating the mechanisms and as the genetic resources that introduce these traits.

Download Full-text