Factors Affecting the In Vitro Production of Volatile Fatty Acids by Mixed Bacterial Populations from the Bovine Rumen

1989 ◽  
Vol 67 (6) ◽  
pp. 1593 ◽  
Author(s):  
J. P. Peters ◽  
J.A.Z. Leedle ◽  
J. B. Paulissen
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
In Vitro Production ◽  
Bacterial Populations ◽  
Factors Affecting ◽  
Bovine Rumen ◽  
Vitro Production

The in vitro production, by rumen micro-organisms, of volatile fatty acids from cellulose and hemicellulose labelled with 14C

1961 ◽  
Vol 56 (1) ◽  
pp. 131-136 ◽  
Author(s):  
I. H. Bath ◽  
M. J. Head
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
New Technique ◽  
In Vitro Production ◽  
End Products ◽  
A New Technique ◽  
Micro Organisms ◽  
Vitro Production

1. A new technique has been used to study the fermentation of hemicellulose and α-cellulose in vitro. This involved the use of 14C-labelled carbohydrates fermented in the presence of normal herbage material in an artificial rumen.2. A method of growing grass in an atmosphere of 14CO2, its fractionation into hemicellulose and α-cellulose and the analysis of the labelled V.F.A. end-products are described.

scholarly journals Profiles of Odd- and Branched-Chain Fatty Acids and Their Correlations With Rumen Fermentation Parameters, Microbial Protein Synthesis and Bacterial Populations Based on Pure Carbohydrate Incubation in Vitro

2020 ◽  
Author(s):  
Hangshu Xin ◽  
Xin Liu ◽  
Xin Jiang ◽  
Chunlong Liu ◽  
Shuzhi Zhang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Cellulolytic Bacteria ◽  
Bacterial Populations ◽  
Branched Chain Fatty Acids ◽  
Degrading Bacteria ◽  
Branched Chain ◽  
Fermentation Parameters ◽  
Chain Fatty Acids

Abstract Background: The objectives of this study were to evaluate the profiles of odd- and branched-chain fatty acids (OBCFA; including C15:0, iso-C15:0, anteiso-C15:0, iso-C16:0, C17:0, iso-C17:0 and anteiso-C17:0) during pure carbohydrates incubation in vitro and whether they correlated with ruminal fermentation parameters, microbial crude protein (MCP) synthesis, and bacterial populations. The pure substrates containing five different ratios of fiber and starch (F:S; 0:100, 25:75, 50:50, 75:25 and 100:0) were incubated for 6 h, 12 h, 18 h and 24 h. Results: Except iso-C17:0, OBCFA concentrations were interacted by F:S and incubation time. The highest concentration of total OBCFA was found in the fermented mixture after 24 h of incubation when the F:S = 0:100; while the lowest level was 1.65 mg/g DM produced after 6 h of incubation with F:S = 50:50. The concentrations of total volatile fatty acids (TVFA) and MCP remarkably decreased linearly as the inclusion of fiber in the substrates increased, as expected. The proportions of investigated cellulolytic bacteria in our study were increased linearly (or linearly and quadratically) while those of R. amylophilus and S. bovis were decreased as fiber inclusion increased. The correlation analysis indicated that iso-C16:0 concentration might have potential as a marker of productions of TVFA and MCP with ρ being 0.78 and 0.82 respectively. Compared to starch degrading bacteria, cellulolytic bacteria had more correlations with OBCFA profiles, and the strongest association was found on the population of R. flavefaciens with C15:0 concentration (ρ = 0.70). Conclusions: Our study shows there might be scope for iso-C16:0 to predict rumen productions of VFA and MCP. Notedly, this is the first paper reporting linkage of OBCFA with rumen function based on pure carbohydrate in vitro incubation, which would avoid confounding interference from dietary protein and fat presence. However, more in-depth experiments are needed to substantiate the current findings.

scholarly journals Factors affecting the in vitro production of bovine embryos in a commercial program

2021 ◽  
Vol 10 (2) ◽  
pp. e16110212264
Author(s):  
Bárbara Gomes Rodrigues Nogueira ◽  
Lilian Franscico Arantes de Souza ◽  
Raquel Zaneti Puelker ◽  
Inês Cristina Giometti ◽  
Sheila Merlo Garcia Firetti ◽  
...  
Keyword(s):  
Bos Indicus ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Chi Square ◽  
Factors Affecting ◽  
Group Frequency ◽  
Sexed Semen ◽  
Using Data ◽  
Vitro Production

Variability in the production of bovine blastocysts per session of ultrasound-guided follicularaspiration(OPU) is still an obstacle in commercial production.Thus, the objective of the current work was to verify factors that influence in vitro embryo production (PIVE), using data from a commercial laboratory.Data from 2014 to 2016 were analyzed, referring to 799 OPU sessions of adult bovine females of the breeds: Nelore (n=83), Girolando (n=73), Brangus (n=49), HPB (n=20), and Senepol (n=10).The influence of five variables was analyzed: breed, genetic group, frequency of aspiration, type of semen (sexed/conventional), and seasonality (spring/summer vs. autumn/winter) on the rate of viable oocytes, cleavage, and blastocyst production (bl).The protocols for the in vitroproduction of embryos (IVP) followed the routine established by the partner laboratory of the present study. The statistical analysis of the data was performed using SAS, with the Chi-square test.The Girolando and Nelore breeds had a higher (p=0.0001) number of blastocysts/OPU session, as well as the Bos indicus blood group.Blastocyst production was higher (p=0.0059) with sexed semen compared to conventional semen (6.6 x 5.2 bl/OPU session); a higher frequency of aspirations also increased (p=0.005) the number of bl/OPU session (7.7 x 5.9).We conclude that the in vitro production of bovine embryos is influenced by the analyzed factors and knowledge of these variables could guide the commercial use of OPU-PIVE. 

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