scholarly journals The effect of ADAMTS1, RBFOX2, THBS1 and WHSC1 gene expression on the formation of a radiation-induced cellular response

2020 ◽  
pp. 85-87
Author(s):  
Р.Р. Савченко ◽  
С.А. Васильев ◽  
В.С. Фишман ◽  
Е.С. Сухих ◽  
О.В. Грибова ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cellular Response ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Model Systems ◽  
Human Peripheral Blood Lymphocytes ◽  
Genes Expression ◽  
Radiation Induced ◽  
Potential Opportunity

Проведен анализ выживаемости клеток, оценка уровня фокусов белков репарации ДНК и частоты радиационно-индуцированных микроядер в условиях дифференциальной экспрессии генов ADAMTS1, RBFOX2, THBS1 и WHSC1 в модельных системах in vitro и в лимфоцитах периферической крови человека. Полученные результаты указывают на потенциальную возможность использования уровня экспрессии данных генов для выявления индивидов с повышенной радиочувствительностью. In this study, we investigated the cell survival, the DNA repair foci level and the radiation-induced micronuclei frequency in the model systems in vitro and in the human peripheral blood lymphocytes with differential expression of ADAMTS1, RBFOX2, THBS1 and WHSC1 genes. The results indicate the potential opportunity of using these genes expression level to identify the individuals with increased radiosensitivity.

scholarly journals The impact of astaxanthin on radiation-induced chromosome aberrations in human peripheral blood lymphocytes in vitro

2016 ◽  
Vol 14 (1) ◽  
pp. 52-57
Author(s):  
M. A. Pilinska ◽  
D. A. Kurinnyi ◽  
S. R. Rushkovsky ◽  
O. B. Dybska
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Metaphase Chromosomes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Radiation Induced ◽  
The Impact

Aim. Research objective is to establish the possibility of modifying the astaxanthin (a carotenoid from a xanthophyll group) radiation-induced cytogenetic effects in human peripheral blood lymphocytes (PBLs) in vitro. Methods. The cultivation of PBLs from four conventionally healthy volunteers, the preparation and analysis of uniformly stained slides of metaphase chromosomes. Astaxanthin in final concentrations of 2, 10 and 20 µg/ml was added into the culture of PBL prior to the incubation before irradiation with γ-quanta in a dose of 1 Gy. Results. Astaxanthin did not affect the level and spectrum of chromosome damage in non-irradiated PBLs both in individual persons, and along the group on average (P>0.05), indicating a lack of mutagenic activity. The effect of astaxanthin at a concentration of 20 μg/ml on irradiated PBLs resulted in a significant reduction of radioinduced cytogenetic effect in all donors. Medium-group level of chromosome aberrations decreased almost 3 times and was characterized by statistically significant (P<0.001) decrease in frequency of chromosomal type aberrations due to the classical unstable cytogenetic markers of radiation effect, dicentrics and ring chromosomes. Conclusions. Astaxanthin at a concentration of 20 µg/ml was found to reduce the mutagenic effect of ionizing radiation, thus suggesting its powerful radioprotective potential. Keywords: astaxanthin, culture of human peripheral blood lymphocytes, radiation mutagenesis, chromosome aberrations, radioprotective effect.

scholarly journals In vitro cytogenetic toxicity of bezafibrate in human peripheral blood lymphocytes

2017 ◽  
Vol 69 (4) ◽  
pp. 579-589 ◽  
Author(s):  
M. Topaktas ◽  
N. E. Kafkas ◽  
S. Sadighazadi ◽  
E. S. Istifli
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes
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