0141 - Characterization of bacterial populations hosting plasmids in marine sponges from Taiwan

2018 ◽  
Author(s):  
Vanessa Oliveira Oliveira
Keyword(s):  
Marine Sponges ◽  
Bacterial Populations

scholarly journals Molecular analysis of the microflora in chronic venous leg ulceration

2003 ◽  
Vol 52 (4) ◽  
pp. 365-369 ◽  
Author(s):  
K.E. Hill ◽  
C.E. Davies ◽  
M.J. Wilson ◽  
P. Stephens ◽  
K.G. Harding ◽  
...  
Keyword(s):  
Individual Species ◽  
Venous Leg Ulcer ◽  
Bacterial Populations ◽  
Cultural Methods ◽  
Rdna Sequences ◽  
Oral Microflora ◽  
16S Rdna Sequences ◽  
First Time

There is growing evidence to suggest that the resident microflora of chronic venous leg ulcers impairs cellular wound-healing responses, thereby playing an important role in maintaining the non-healing phenotype of many of these wounds. The significance of individual species of bacteria will remain unclear until it is possible to characterize fully the microflora of such lesions. The limitations and biases of culture-based microbiology are being realized and the subsequent application of molecular methods is revealing greater diversity within mixed bacterial populations than that demonstrated by culture alone. To date, this approach has been limited to a small number of systems, including the oral microflora. Here, for the first time, the comprehensive characterization of the microflora present in the tissue of a chronic venous leg ulcer is described by the comparison of 16S rDNA sequences amplified directly from the wound tissue with sequences obtained from bacteria that were isolated by culture. The molecular approach demonstrated significantly greater bacterial diversity than that revealed by culture. Furthermore, sequences were retrieved that may possibly represent novel species of bacteria. It is only by the comprehensive analysis of the wound microflora by both molecular and cultural methods that it will be possible to further our understanding of the role of bacteria in this important condition.

Characterization of geochemical constituents and bacterial populations associated with As mobilization in deep and shallow tube wells in Bangladesh

2009 ◽  
Vol 43 (6) ◽  
pp. 1720-1730 ◽  
Author(s):  
Nora B. Sutton ◽  
Geert M. van der Kraan ◽  
Mark C.M. van Loosdrecht ◽  
Gerard Muyzer ◽  
Johannes Bruining ◽  
...  
Keyword(s):  
Bacterial Populations ◽  
Tube Wells

Microbiological Characterization of Human Milk1

1977 ◽  
Vol 40 (9) ◽  
pp. 614-616 ◽  
Author(s):  
A. GAVIN ◽  
K. OSTOVAR
Keyword(s):  
Staphylococcus Aureus ◽  
Breast Feeding ◽  
Staphylococcus Epidermidis ◽  
Lactobacillus Acidophilus ◽  
Bacterial Population ◽  
Streptococcus Salivarius ◽  
Streptococcus Mitis ◽  
Bacterial Populations ◽  
Milk Samples

Milk samples of five breast-feeding mothers were studied for bacterial population, flora, and source. In most instances, samples taken at postfeeding contained higher bacterial populations than prefeeding samples. Staphylococcus epidermidis was the predominant organism isolated from 100% of the samples. Increases were noticed in the appearance of Streptococcus mitis, Gaffkya tetragena, Streptococcus salivarius, Staphylococcus aureus, as well as Lactobacillus acidophilus, in the postfeeding samples. Main sources of bacteria were found to be the infant's mouth and maternal skin.

Micro- and nano-structural characterization of six marine sponges of the class Demospongiae

2016 ◽  
Vol 45 (8) ◽  
pp. 831-842 ◽  
Author(s):  
Elif Hilal Şen ◽  
Semra Ide ◽  
Sevgi Haman Bayari ◽  
Malcolm Hill
Keyword(s):  
Structural Characterization ◽  
Marine Sponges

Characterization of serine/threonine protein phosphatases in RINm5F insulinoma cells

1993 ◽  
Vol 13 (6) ◽  
pp. 349-358 ◽  
Author(s):  
Åke Sjöhom ◽  
Richard E. Honkanen ◽  
Per-Olof Berggren
Keyword(s):  
Okadaic Acid ◽  
Potent Inhibitor ◽  
Protein Phosphatases ◽  
Marine Sponges ◽  
Rinm5f Cell ◽  
Ic50 Values ◽  
Marine Dinoflagellates ◽  
Insulinoma Cells

This study investigates the occurrence and regulation of serine/threonine protein phosphatases (PPases) in insulin-secreting RINm5F insulinoma cells. PPases types 1 and 2A were identified in crude RINm5F cell homogenates by both enzymatic assay and Western blot analysis. We then characterized and compared the inhibitory actions of several compounds isolated from cyanobacteria, marine dinoflagellates and marine sponges, (viz. okadaic acid, microcystin-LR, calyculin-A and nodularin) cation-independent PPase activities in RINm5F cell homogenates. It was found that okadaic acid was the least potent inhibitor (IC50 ≈ 10−9M, IC100 ≈ 10−6M), while the other compounds exhibited IC50 values of ≈ 5·10−10 M and IC100 ≈ 5·10−9 M. The findings indicate that the inhibitory substances employed in this study may be used pharmacologically to investigate the role of serine/threonine PPases in RINm5F cell insulin secretion, a process that is likely to be regulated to a major extent by protein phosphorylation.

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