Detection of rare autoreactive T cell subsets in patients with pemphigus vulgaris by the CD154 activation assay

2021 ◽  
Author(s):  
Alexandra Polakova
Keyword(s):  
T Cell ◽  
Pemphigus Vulgaris ◽  
T Cell Subsets ◽  
Autoreactive T Cell ◽  
Activation Assay

scholarly journals 026 Detection of rare autoreactive T cell subsets in patients with pemphigus vulgaris by the CD154 activation assay

2021 ◽  
Vol 141 (10) ◽  
pp. S153
Author(s):  
A. Polakova ◽  
L. Kauter ◽  
A. Ismagambetova ◽  
C. Möbs ◽  
C. Hudemann
Keyword(s):  
T Cell ◽  
Pemphigus Vulgaris ◽  
T Cell Subsets ◽  
Autoreactive T Cell ◽  
Activation Assay

scholarly journals Autoreactive CD1b-restricted T cells: a new innate-like T-cell population that contributes to immunity against infection

Blood ◽  
2011 ◽  
Vol 118 (14) ◽  
pp. 3870-3878 ◽  
Author(s):  
Sha Li ◽  
Hak-Jong Choi ◽  
Kyrie Felio ◽  
Chyung-Ru Wang
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Subsets ◽  
Autoreactive T Cell ◽  
Suitable Animal Model ◽  
Lipid Antigen ◽  
Listeria Infection ◽  
Express Group ◽  
Group 1

Abstract Group 1 CD1 (CD1a, -b, and -c) presents self and foreign lipid antigens to multiple T-cell subsets in humans. However, in the absence of a suitable animal model, the specific functions and developmental requirements of these T cells remain unknown. To study group 1 CD1-restricted T cells in vivo, we generated double transgenic mice (HJ1Tg/hCD1Tg) that express group 1 CD1 molecules in a similar pattern to that observed in humans (hCD1Tg) as well as a TCR derived from a CD1b-autoreactive T-cell line (HJ1Tg). Using this model, we found that similar to CD1d-restricted NKT cells, HJ1 T cells exhibit an activated phenotype (CD44hiCD69+CD122+) and a subset of HJ1 T cells expresses NK1.1 and is selected by CD1b-expressing hematopoietic cells. HJ1 T cells secrete proinflammatory cytokines in response to stimulation with CD1b-expressing dendritic cells derived from humans as well as hCD1Tg mice, suggesting that they recognize species conserved self-lipid antigen(s). Importantly, this basal autoreactivity is enhanced by TLR-mediated signaling and HJ1 T cells can be activated and confer protection against Listeria infection. Taken together, our data indicate that CD1b-autoreactive T cells, unlike mycobacterial lipid antigen-specific T cells, are innate-like T cells that may contribute to early anti-microbial host defense.

scholarly journals Conversion of Monophasic to Recurrent Autoimmune Disease by Autoreactive T Cell Subsets

2003 ◽  
Vol 171 (10) ◽  
pp. 5624-5630 ◽  
Author(s):  
Hui Shao ◽  
Song Lei ◽  
Sheher L. Sun ◽  
Henry J. Kaplan ◽  
Deming Sun
Keyword(s):  
T Cell ◽  
Autoimmune Disease ◽  
T Cell Subsets ◽  
Autoreactive T Cell

scholarly journals TCR repertoire analysis reveals effector memory T cells differentiation into Th17 cells in rheumatoid arthritis

2019 ◽  
Author(s):  
Xu Jiang ◽  
Shi-yu Wang ◽  
Chen Zhou ◽  
Jing-hua Wu ◽  
Yu-hao Jiao ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cells ◽  
T Cell ◽  
T Cell Receptor ◽  
Th17 Cells ◽  
Cell Receptor ◽  
T Cell Subsets ◽  
Effector Memory ◽  
Systemic Autoimmune Disease ◽  
Autoreactive T Cell

AbstractThe pathogenesis of rheumatoid arthritis (RA), a systemic autoimmune disease characterized by autoreactive T-cell accumulation and pro-inflammatory cytokine overproduction, is unclear. Systematically addressing T-cell receptor (TCR) repertoires of different CD4+ T-cell subsets could help understand RA pathogenesis. Here, peripheral CD4+ T cells from treatment-naïve RA patients and healthy controls were sorted into seven subsets including naïve, effector, central memory, effector memory (EMT), Th1, Th17, and regulatory T cells. T-cell receptor β chain repertoires were then analyzed by next-generation sequencing. We identified T-cell clonal expansion in EMT and Th17 cells, with highly similar TCR repertoires between them. Ex vivo experiments demonstrated the preferred differentiation from EMT to Th17 cells in RA. Moreover, TCR diversity in subsets including Th17 was negatively correlated with RA disease activity indices such as C-reactive protein and erythrocyte sedimentation rate. Thus, shared and abnormally expanded EMT and Th17 TCR repertoires might be pivotal for RA pathogenesis.

Alanine-substituted peptide ligands differ greatly in their ability to activate autoreactive T-cell subsets specific for the wild-type peptide

1999 ◽  
Vol 99 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Deming Sun ◽  
Christopher Coleclough ◽  
Rong Ji ◽  
Xianzhen Hu ◽  
John N Whitaker
Keyword(s):  
T Cell ◽  
Peptide Ligands ◽  
T Cell Subsets ◽  
Wild Type ◽  
Autoreactive T Cell

HLA-E restricted CD8+ T cell subsets are phenotypically altered in multiple sclerosis patients

2013 ◽  
Vol 20 (7) ◽  
pp. 790-801 ◽  
Author(s):  
Kim Pannemans ◽  
Bieke Broux ◽  
An Goris ◽  
Bénédicte Dubois ◽  
Tom Broekmans ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
T Cells ◽  
T Cell ◽  
B Cells ◽  
T Cell Subsets ◽  
Regulatory Role ◽  
T And B Cells ◽  
Autoreactive T Cell ◽  
Multiple Sclerosis Patients

Background: The importance of Qa-1 restricted CD8+ T cells in regulating autoreactive T cell responses has been demonstrated in animal models for autoimmune disorders, including multiple sclerosis (MS). Objective: We hypothesize that their human variant, HLA-E restricted CD8+ T cells, fulfills a similar regulatory role in man and that these cells are of importance in MS. Methods: A large cohort of MS patients and healthy controls was genotyped for the two known HLA-E polymorphisms. Flow cytometry was used to determine HLA-E expression kinetics and to phenotype HLA-E restricted CD8+ T cells. Immunohistochemistry was performed to investigate HLA-E expression in the central nervous system (CNS) of MS patients. Results: HLA-E is upregulated on immune cells upon in vitro activation and this upregulation is polymorphism-dependent for T and B cells. T and B cells in lesions of MS patients show enhanced HLA-E expression. Furthermore, NKG2C+CD8+ T cells of MS patients have a significantly lower Foxp3 expression, while NKG2A+CD8+ T cells of MS patients produce higher levels of pro-inflammatory cytokines compared to those of healthy individuals. Conclusion: Our study indicates that the HLA-E system is altered in MS and could play a regulatory role in disease.

scholarly journals A novel α/β T-cell subpopulation defined by recognition of EPCR

2021 ◽  
Author(s):  
Elena Erausquin Arrondo ◽  
Maria Moran-Garrido ◽  
Jorge Saiz ◽  
Coral Barbas ◽  
Maria Gilda Dichiara Rodriguez ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Human Peripheral Blood ◽  
Average Frequency ◽  
Lipid Binding ◽  
T Cell Subsets ◽  
Cell Subpopulation ◽  
Endothelial Protein C Receptor ◽  
Autoreactive T Cell ◽  
Self Recognition

T-cell self-recognition of antigen presenting molecules is led by antigen-dependent or independent mechanisms. The endothelial protein C receptor (EPCR) shares remarkable similarity with CD1d, including a lipid binding cavity. We identified EPCR-specific α/β T-cells in human peripheral blood of healthy donors. The average frequency in the CD3+ leukocyte pool is comparable to other autoreactive T-cell subsets that specifically bind MHC-like receptors. Alteration of the EPCR lipid cargo, revealed by X-ray diffraction studies, points to a prevalent, yet not exclusive, lipid-independent self-recognition. In addition, we solve the EPCR lipidome, and detect species not yet described as EPCR ligands. These studies report, for the first time, novel recognition by circulating α/β T-cells and provide grounds for EPCR and lipid mediated T-cell restriction.

Sign in / Sign up

Export Citation Format

Share Document