A Real-Time, Selective, and Low-Cost Strategy for Detection of Trace Level Spike-Protein from SARS-CoV-2 with Application to Cold-Chain Food Quarantine

<div>Utilizing an interdigitated microelectrode chip modified with an antibody probe, and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for detection of trace level spike-protein from SARS-CoV-2 on food. <br></div>

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Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

npj Science of Food ◽

10.1038/s41538-021-00094-3 ◽

2021 ◽

Vol 5 (1) ◽

Author(s):

Jian Zhang ◽

Xin Fang ◽

Yu Mao ◽

Haochen Qi ◽

Jayne Wu ◽

...

Keyword(s):

Real Time ◽

Large Scale ◽

Limit Of Detection ◽

Low Cost ◽

Spike Protein ◽

Cold Chain ◽

Trace Level ◽

Interdigitated Microelectrode ◽

The Cost ◽

Virus Survival

AbstractDue to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.

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Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

10.26434/chemrxiv.13166774 ◽

2020 ◽

Author(s):

Haochen Qi ◽

Jian Zhang ◽

Jayne Wu ◽

Xin Fang ◽

Fangshuo You ◽

...

Keyword(s):

Real Time ◽

Cold Chain ◽

Trace Level ◽

Interdigitated Microelectrode ◽

Interfacial Capacitance

<div>Utilizing an interdigitated microelectrode chip modified with an antibody probe, and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a real-time immunosensor is presented for detection of trace level biomarkers from virus. <br></div>

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Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

10.26434/chemrxiv.13166774.v3 ◽

2020 ◽

Author(s):

Haochen Qi ◽

Jian Zhang ◽

Jayne Wu ◽

Xin Fang ◽

Fangshuo You ◽

...

Keyword(s):

Real Time ◽

Cold Chain ◽

Trace Level ◽

Interdigitated Microelectrode ◽

Interfacial Capacitance

<div>Utilizing an interdigitated microelectrode chip modified with an antibody probe, and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a real-time immunosensor is presented for detection of trace level biomarkers from virus. <br></div>

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Investigation of in-feed organic acids as a low cost strategy to combat Salmonella in grower pigs

Preventive Veterinary Medicine ◽

10.1016/j.prevetmed.2017.02.008 ◽

2017 ◽

Vol 139 ◽

pp. 50-57 ◽

Cited By ~ 17

Author(s):

H. Lynch ◽

F.C. Leonard ◽

K. Walia ◽

P.G. Lawlor ◽

G. Duffy ◽

...

Keyword(s):

Organic Acids ◽

Low Cost ◽

Cost Strategy ◽

Low Cost Strategy

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Performance of the dynamically dimensioned search algorithm: influence of parameter initialization strategy when calibrating a physically based hydrological model

Hydrology Research ◽

10.2166/nh.2017.139 ◽

2017 ◽

Vol 49 (4) ◽

pp. 971-988 ◽

Cited By ~ 5

Author(s):

Franck Lespinas ◽

Ashu Dastoor ◽

Vincent Fortin

Keyword(s):

Search Algorithm ◽

Low Cost ◽

Initial Parameter ◽

Cost Strategy ◽

Physically Based ◽

Physically Based Models ◽

Calibration Solution ◽

Low Cost Strategy ◽

Parameter Values ◽

Initialization Strategy

Abstract This study presents an evaluation of the performance of the dynamically dimensioned search (DDS) algorithm when calibrating the hydrological component of the Visualizing Ecosystems for Land Management Assessments (VELMA) ecohydrological model. Two calibration strategies were tested for the initial parameter values: (1) a ‘high-cost strategy’, where 100 sets of initial parameter values were randomly chosen within the overall parameter space, and (2) a ‘low-cost strategy’, where a unique set of initial parameter values was derived from the available field data. Both strategies were tested for six different values of the maximum number of model evaluations ranging between 100 and 10,000. Results revealed that DDS is able to converge rapidly to a good parameter calibration solution of the VELMA hydrological component regardless of the parameter initialization strategy used. The accuracy and convergence efficiency of the DDS algorithm were, however, slightly better for the low-cost strategy. This study suggests that initializing the parameter values of complex physically based models using information on the watershed characteristics can increase the efficiency of the automatic calibration procedures.

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Induction motor downsizing as a low-cost strategy to save energy

Journal of Cleaner Production ◽

10.1016/j.jclepro.2011.11.014 ◽

2012 ◽

Vol 24 ◽

pp. 117-131 ◽

Cited By ~ 25

Author(s):

Fernando J.T.E. Ferreira ◽

Aníbal T. de Almeida

Keyword(s):

Induction Motor ◽

Low Cost ◽

Cost Strategy ◽

Low Cost Strategy ◽

Save Energy

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Business Strategy, State-Owned Equity and Cost Stickiness: Evidence from Chinese Firms

Sustainability ◽

10.3390/su12051850 ◽

2020 ◽

Vol 12 (5) ◽

pp. 1850

Author(s):

Tingyong Zhong ◽

Fangcheng Sun ◽

Haiyan Zhou ◽

Jeoung Yul Lee

Keyword(s):

Business Strategy ◽

Low Cost ◽

Differentiation Strategy ◽

Cost Strategy ◽

Cost Behavior ◽

Low Cost Strategy ◽

The Cost ◽

The Relationship ◽

Management Expectations ◽

Cost Stickiness

This paper investigates the relationship between business strategy and cost stickiness under different ownership. Using the data from listed firms in China from 2002 to 2015, we find that first, firms with different strategies exhibit different cost behavior. The cost stickiness of choosing a differentiation strategy is higher than that of choosing a low-cost strategy. Second, management expectations will affect cost stickiness. Optimistic expectations will increase cost stickiness, while pessimistic expectations will reduce cost stickiness. Third, management expectations can adjust the relationship between business strategy and cost stickiness in terms of government-created advantages (GCAs). If management expectations tend to be optimistic, the cost stickiness is higher with a differentiation strategy than with a low-cost strategy. If management expectations tend to be pessimistic, then cost stickiness is higher with a low-cost strategy than with a differentiation strategy. Finally, the state-owned equity affects the extent of the effect of a differentiation strategy on cost stickiness. State-owned firms, which receive more GCAs than non-state-owned firms, have stronger cost stickiness than non-state-owned firms, even if both categories of firms use more differentiation strategy.

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