scholarly journals Animal Species Identification of Meat Using MALDI-TOF Mass Spectrometry

2021 ◽  
Author(s):  
Jörg Rau ◽  
Ekkehard Hiller ◽  
Annegret Männig ◽  
Martin Dyk ◽  
Olivera Wenninger ◽  
...  
Keyword(s):  
Species Identification ◽  
Reliable Method ◽  
Animal Species ◽  
Reference Database ◽  
Species Differentiation ◽  
Food Control ◽  
Tryptic Digest ◽  
Maldi Tof ◽  
Development And Validation ◽  
Extensive Reference

<p>We describe the animal species identification of meat using MALDI-TOF mass spectroscopy including the development and validation of a reliable method, qualified for use in the accredited official food-control laboratory.</p> <p>Previous publications had shown the potential of MALDI-TOF MS for animal species differentiation of several kind of food, including meat. Our aim was to establish a rapid and reliable method by means of a simplified sample preparation without prior tryptic digest, an existing popular MALDI system, an independent extensive reference database, and an adequate validation concept. In contrast to the previous works, we consequently use the MALDI user platform “MALDI-UP” to give other food control laboratories the possibility of exchanging reference and validation spectra.<br></p>

scholarly journals Animal Species Identification of Meat Using MALDI-TOF Mass Spectrometry

2021 ◽  
Author(s):  
Jörg Rau ◽  
Ekkehard Hiller ◽  
Annegret Männig ◽  
Martin Dyk ◽  
Olivera Wenninger ◽  
...  
Keyword(s):  
Species Identification ◽  
Reliable Method ◽  
Animal Species ◽  
Reference Database ◽  
Species Differentiation ◽  
Food Control ◽  
Tryptic Digest ◽  
Maldi Tof ◽  
Development And Validation ◽  
Extensive Reference

<p>We describe the animal species identification of meat using MALDI-TOF mass spectroscopy including the development and validation of a reliable method, qualified for use in the accredited official food-control laboratory.</p> <p>Previous publications had shown the potential of MALDI-TOF MS for animal species differentiation of several kind of food, including meat. Our aim was to establish a rapid and reliable method by means of a simplified sample preparation without prior tryptic digest, an existing popular MALDI system, an independent extensive reference database, and an adequate validation concept. In contrast to the previous works, we consequently use the MALDI user platform “MALDI-UP” to give other food control laboratories the possibility of exchanging reference and validation spectra.<br></p>

Rapid animal species identification of feta and mozzarella cheese using MALDI-TOF mass-spectrometry

Food Control ◽  
2020 ◽  
Vol 117 ◽  
pp. 107349 ◽  
Author(s):  
Jörg Rau ◽  
Nadine Korte ◽  
Martin Dyk ◽  
Olivera Wenninger ◽  
Pat Schreiter ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Species Identification ◽  
Animal Species ◽  
Mozzarella Cheese ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samples

2020 ◽  
Vol 145 ◽  
pp. 01020
Author(s):  
Chen Yun-xia ◽  
Xue Xiao-ming ◽  
Huang Ya-lin ◽  
Zhou Yong-wu ◽  
Hou Sen-lin ◽  
...  
Keyword(s):  
Species Identification ◽  
Biological Samples ◽  
Animal Species ◽  
Simultaneous Detection ◽  
Species Level ◽  
Reference Database ◽  
Target Classification ◽  
Identification Rate ◽  
Dna Metabarcoding ◽  
Mixed Samples

In this study, the second-generation high-throughput sequencing and DNA barcoding were combined to manually prepare multi-species mixed samples, and the mitochondrial gene CO І was used as a barcode to simultaneously identify the animal species in the mixed samples and identify endangered species. The results showed that under the family and genus level, the simultaneous detection rate of the species in the mixed samples was as high as 100%, and the species identification rate was as high as 89% at the species level, and with high sensitivity, as little as 1% of the trace species could be detected. However, nearly 30% of non-target classification annotations appeared at the species level. It can be concluded that the mini CO I barcoding can be applied to the simultaneous identification of animal species in mixed biological samples, and the species identification rate is high. Non-target classification match existing at the species level can be further improved by increasing the length of the barcoding, improving the sequencing technology, reference database and so on. In this study, DNA metabarcoding technology was used to evaluate the feasibility of identification of endangered animals in multi-species mixed biological samples with CO І, in order to lay a preliminary foundation for the advancement of DNA metabarcoding method in the field of wildlife forensic identification.

scholarly journals Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 366
Author(s):  
Helena Berlamont ◽  
Chloë De Witte ◽  
Sofie De Bruyckere ◽  
James G. Fox ◽  
Steffen Backert ◽  
...  
Keyword(s):  
Species Identification ◽  
Growth Conditions ◽  
Reference Database ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Animal Pathogens ◽  
H Pylori ◽  
Specific Species ◽  
Individual Mass ◽  
Tof Ms

Gastric helicobacters (Helicobacter (H.) pylori and non-H. pylori Helicobacter species (NHPHs)) colonize the stomach of humans and/or animals. Helicobacter species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, Helicobacter species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric Helicobacter isolates of 10 different Helicobacter species using MALDI-TOF MS in order to establish a more elaborate Helicobacter reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores ≥2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related Helicobacter species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric Helicobacter species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account.

Rapid species identification of seafood spoilage and pathogenic Gram-positive bacteria by MALDI-TOF mass fingerprinting

2011 ◽  
Vol 32 (21) ◽  
pp. 2951-2965 ◽  
Author(s):  
Karola Böhme ◽  
Inmaculada C. Fernández-No ◽  
Jorge Barros-Velázquez ◽  
Jose M. Gallardo ◽  
Benito Cañas ◽  
...  
Keyword(s):  
Species Identification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Maldi Tof

Species identification of Streptococcus bovis group isolates causing bacteremia: a comparison of two MALDI-TOF MS systems

2017 ◽  
Vol 88 (1) ◽  
pp. 23-25 ◽  
Author(s):  
Charlotte N. Agergaard ◽  
Elisa Knudsen ◽  
Rimtas Dargis ◽  
Xiaohui C. Nielsen ◽  
Jens J. Christensen ◽  
...  
Keyword(s):  
Species Identification ◽  
Streptococcus Bovis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Development and validation of a reliable method for studying the distribution pattern for opiates metabolites in brain

2013 ◽  
Vol 73 ◽  
pp. 125-130 ◽  
Author(s):  
Katia Guerrini ◽  
Antonella Argo ◽  
Cristina Borroni ◽  
Daria Catalano ◽  
Lucia Dell’Acqua ◽  
...  
Keyword(s):  
Distribution Pattern ◽  
Reliable Method ◽  
Development And Validation

scholarly journals Relevance of wing morphology in distinguishing and classifying genera and species of Stenogastrinae wasps

2011 ◽  
Vol 80 (3) ◽  
pp. 191-199 ◽  
Author(s):  
David Baracchi ◽  
Leonardo Dapporto ◽  
Stefano Turillazzi
Keyword(s):  
Geometric Morphometrics ◽  
Species Identification ◽  
Reliable Method ◽  
Wing Morphology ◽  
Wing Vein ◽  
Statistical Assessment ◽  
Genus Level ◽  
Nesting Material ◽  
First Time

The phylogeny of the Stenogastrinae wasps is still under discussion and their systematic incomplete. In the present work we used geometric morphometrics, a technique based on a rigorous statistical assessment of shape, to compare the forewings of fifteen species of Stenogastrinae wasps belonging to four different genera to ascertain whether this approach may be used as a reliable method in the study of the taxonomy of the group. The results show that the wing vein junctions can be diagnostic for both genus and species identification. For the first time in this subfamily, we propose a phylogenetic classification of the species based on wing morphology that largely agrees with the cladistic data available at genus level and reflects the differences among species in terms of nesting material and architecture of their nest.

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