scholarly journals RP-HPLC-DAD determination and quantification of Quercetin and Luteolin in plant extracts of Merremia aegyptia and Merremia dissecta

2020 ◽  
Vol 11 (3) ◽  
pp. 4660-4664
Author(s):  
Ridhi Joshi ◽  
Rishikesh Meena ◽  
Preeti Mishra ◽  
Vidya Patni
Keyword(s):  
High Performance ◽  
Food Additives ◽  
High Performance Liquid Chromatographic ◽  
Pharmaceutical Products ◽  
Array Detector ◽  
Diode Array Detector ◽  
Methanolic Extracts ◽  
Rp Hplc ◽  
Liquid Chromatographic ◽  
Extracting Solvent

Medicinal plants produce various useful metabolites such as alkaloids, flavonoids, tannins etc those are widely used for the preparation of various pharmaceutical products, or as food additives. A simple and precise reverse phase high performance liquid chromatographic (RP-HPLC) seperation method has been developed for determination and quantification of the flavonoids, quercetin and luteolin simultaneously, from methanolic extracts of Merremia aegyptia and Merremia dissecta after optimization of extracting solvent and chromatographic conditions through HPLC coupled to a Diode Array Detector(DAD). HPLC analysis estimated contents of the quercetin to be 20 ng/µl in M. aegyptia stem and 13.2 ng/ µl in M. dissecta callus whereas luteolin was found to be 0.4 mg/ml in M. dissecta callus. From the previous published literature, it appears that this is the first report of quantification of these flavonoid compounds as they have not been reported earlier in both the species under study.

HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC - DIODE ARRAY DETECTOR METHOD FOR SIMULTANEOUS ESTIMATION OF ESCITALOPRAM OXALATE AND FLUPENTIXOL DIHYDROCHLORIDE IN COMBINATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (08) ◽  
pp. 61-68
Author(s):  
V. V Gawande ◽  
A. V. Chandewar ◽  
Keyword(s):  
High Performance ◽  
Standard Addition ◽  
High Performance Liquid Chromatographic ◽  
Array Detector ◽  
Simultaneous Estimation ◽  
Diode Array ◽  
Diode Array Detector ◽  
Detector Method ◽  
High Degree ◽  
Liquid Chromatographic

The proposed work involves High Performance Liquid Chromatographic – Diode Array Detector method for estimation of escitalopram oxalate and flupentixol dihydrochloride in combination. The analytes were resolved on Waters C18 Xterra column (50 × 4.6 mm id, particle size 3.5 μm) with mobile phase composition of water (containing 0.2 % triethylamine pH adjusted to 2.5 with orthophosphoric acid): acetonitrile: methanol (62:28:10 % v/v/v). The flow rate kept was 1.0 mL/min opting isocratic mode and eluents were tracked down at 230 nm. The retention times for both the drugs were 1.38±0.21 and 2.98±0.41, respectively. Standard addition method was employed for the analysis of flupentixol dihydrochloride. ICH guidelines were followed to validate the method. The method assured linearity in concentration range of 50-150 μg/mL and 2.5-7.5 μg/mL for ESC and FLU, respectively. The method assured high degree of precision and accuracy. The method was proved to be robust by assessing robustness parameters.

Development and Validation of the Analytical method for the estimation of a combination of 5-fluorouracil and Imiquimod by RP-HPLC

2021 ◽  
pp. 3313-3318
Author(s):  
Bhupender Tomar ◽  
Ankita Sharma ◽  
Inder Kumar ◽  
Sandeep Jain ◽  
Pallavi Ahirrao
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
High Performance Liquid Chromatographic ◽  
Simultaneous Estimation ◽  
Limit Of Quantification ◽  
Pharmaceutical Ingredients ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Development And Validation ◽  
Liquid Chromatographic

A simple, precise, and accurate reverse phase high performance liquid chromatographic method (RP-HPLC) was developed and validated for the estimation of the combination of 5- Fluorouracil (5-FU) and Imiquimod in active pharmaceutical ingredients (APIs). The method was carried out on Phenomenex C18 (250 × 4.6mm I.D., 5𝜇m) using isocratic elution mode. The mobile phase was used as Acetonitrile: 10mM potassium dihydrogen orthophosphate: triethylamine (40:59.9:0.1, v/v, pH 4.5 with orthophosphoric acid) and Water: ACN (50:50 v/v) was used as a diluent. The concentration of solvents was 1-20µg/ml and the volume of injection was 20µl with the flow rate of 1.2ml/min. The retention times for 5-FU and Imiquimod were found to be 1.9±0.5 and 6.6±0.5 min respectively. The absorption maxima of 5FU and Imiquimod were found 267nm and 227nm respectively. The method was validated as per ICH guidelines. All the data were found within the specified limits. The limit of detection (LOD) and limit of quantification (LOQ) of 5- Fluorouracil were found to be 0.015μg/mL and 0.048 μg/mL, respectively, and Imiquimod was found to be 0.078μg/mL and 0.237μg/mL, respectively. The method developed in the present study was found to be sensitive, specific, and precise and can be applied for the simultaneous estimation of 5-FU and Imiquimod.

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