The estimation of posibility of stearic and oleic acids' TLC-separation with eluents containing surfactants

Myristic Acid ◽

Mobile Phase ◽

Hydrophobic Interactions ◽

Cetylpyridinium Chloride ◽

Tween 80 ◽

Mobile Phases ◽

The possibility of separation of oleic and stearic acids with mobile phases on the basis of surfactants was estimated by the method of a thin-layer chromatography. The influence of surfactant's type and concentration on the retention parameters of the studied acids when using of normal-phase plates for a thin-layer chromatography of Sorbfil UV-254 is investigated in the work. The influence on mobility of oleic and stearic acids of the anionic sodium n-dodecylsulphate, cationic cetylpyridinium chloride and non-ionic Tween-80 in the range of concentration from 1·10-5 mol/l to 0.1 mol/l is estimated. The mixed mobile phases on the basis of two last surfactants taken in various concentrations are also investigated. The рН of eluents was varied in the range from 1 to 9. The addition of 2-propanol into the mobile phase containing surfactant didn't lead to improvement of acids' distribution efficiency. The best results have been received with the eluent, containing 1·10-5 mol/l of cetylpyridinium chloride and boric buffer solution with pH 9. Preliminary modification of silica gel surface with cetylpyridinium chloride did not change the results of chromatography. For clarification of relation of acids' retention with a length of a hydrophobic fragment, and, therefore, a contribution of hydrophobic interactions, in system with the mixed mobile phase on the basis of cetylpyridinium chloride and Tween-80 at pH 3 formic acid, acetic acid, propionic acid, hexanoic acid and myristic acid were analyzed. The smallest mobility was observed for myristic acid (lgPo/w=6.09±0.19) that indicates very strong hydrophobic interactions of acid with the surface of silica gel modified by surfactants. Such interactions are the main reason of bad mobility of studied oleic (lgPo/w=7.7±0.2) and stearic (lgPo/w=8.22±0.19) acids in system with the surfactant-containing eluents. For the manifestation of acidic zones after chromatography, a solution of phosphomolybdic acid, a solution of rhodamine B, a solution of sulfuric acid and an iodine chamber were tested. The best visualization of TLC-сhromatogram is obtained after sprinkle the plates by 6% solution of sulfuric acid in ethanol with their subsequent heating at 110 °C.

Use of Nonionic Tween-80 Surfactant Mobile Phase in Thin Layer Chromatography of Heavy Metal Cations

American Journal of Materials Science and Technology ◽

10.7726/ajmst.2013.1002 ◽

2013 ◽

Cited By ~ 1

Author(s):

Sarang Dhote ◽

Lata Deshmukh ◽

L. Paliwal

Keyword(s):

Heavy Metal ◽

Thin Layer ◽

Mobile Phase ◽

Metal Cations ◽

Tween 80 ◽

Heavy Metal Cations ◽

Reverse Phase Thin Layer Chromatography of Some Aroclors, Halowaxes, and Pesticides

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/56.2.367 ◽

1973 ◽

Vol 56 (2) ◽

pp. 367-372

Author(s):

David L Stalling ◽

James N Huckins

Keyword(s):

Thin Layer ◽

Mobile Phase ◽

Gas Liquid Chromatography ◽

Chlorinated Pesticides ◽

Reverse Phase ◽

Mobile Phases ◽

Rf Values ◽

Solvent Systems ◽

Abstract The components of Aroclors® 1232, 1248, 1254, and 1260; Halowaxes® 1099, 1013, and 1014; and several chlorinated pesticides are resolved by reverse phase thin layer chromatography (RPTLC), which permits component separation by partition between a nonpolar stationary phase and a polar mobile phase. Rf values of resulting spots were calculated for 2 of 4 new solvent systems (mobile phases). RPTLC patterns were reproducible and characteristic of each material examined. The spots were recovered from the plates and characterized by gas-liquid chromatography (GLC) and/or GLC-mass spectrometry. In some cases, single GLC peaks of Aroclor standards were resolved into more than one component by RPTLC, whereas some RPTLC spots of Halowaxes were resolved into as many as 4 GLC peaks. The analysis of environmental residues of chlorinated compounds was facilitated by this technique.

A validated method for coumarin quantification in Meliloti herba and its ethanolic extracts using micellar thin-layer chromatography

Annals of Advances in Chemistry ◽

10.29328/journal.aac.1001024 ◽

2021 ◽

Vol 5 (1) ◽

pp. 013-018

Author(s):

Verushkin Aleksey G ◽

Kulikov Artem Yu ◽

Kutsanyan Andranik A

Keyword(s):

Green Chemistry ◽

Thin Layer ◽

Mobile Phase ◽

Tween 80 ◽

Chromatography Method ◽

Ethanolic Extracts ◽

Medical Plant ◽

Good Determination ◽

A micellar thin-layer chromatography method for the quantitative determination and validation of coumarin in Meliloti herba and its ethanolic extracts was developed and validated. For achieving good determination, the mobile phase of 5 x 10-4 mol/L Tween-80 in a mixture propanol-2 – water (5:95 v/v) was used. Densitometric determination was carried out at 275 nm. The calibration curve was linear in the range of 0.1-2.5 μg per band. The proposed method is simple, rapid, precise and accurate; replacing hazardous solvents by greener ones correspond to the modern requirements in “Green chemistry” concepts. The obtained data can be used for the routine analysis of coumarin in medical plant and extracts.