scholarly journals Group A streptococcal antigen detection in school children

1998 ◽  
Vol 4 (2) ◽  
pp. 260-264
Author(s):  
H. Nsanze ◽  
K. Dawson ◽  
A. S. Ameen ◽  
N. Mustafa
Keyword(s):  
School Children ◽  
Group A Streptococcus ◽  
Positive Culture ◽  
Antigen Detection ◽  
Streptococcal Antigen ◽  
Group A ◽  
Culture Negative

The objective of the study was to determine the correlation between group A streptococcal antigen detected from throat swabs with the culture results. A total of 1457 children had two swabs taken simultaneously, and culture and antigen detection were performed. There was a good correlation between antigen detection and isolation rates. In all, 225 strains of group A streptococcus were isolated;53 [57.6%] were from the 92 children with high antigen positivity, 68 [55.7%] were from the 122 children with medium antigen positivity and 77 [25.4%] were from 303 children with low antigen positivity;only 27 [2.9%] were from the 940 children with no antigen detected. We postulate that those who are antigen-positive, culture-negative carry the organisms in their throats, but they may be missed on culture because of the small number carried

scholarly journals Sequential Quadriplex Real-Time PCR for Identifying 20 Common emm Types of Group A Streptococcus

2020 ◽  
Vol 59 (1) ◽  
pp. e01764-20
Author(s):  
Srinivasan Velusamy ◽  
Katherine Jordak ◽  
Madeline Kupor ◽  
Sopio Chochua ◽  
Lesley McGee ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Group A Streptococcus ◽  
High Specificity ◽  
The United States ◽  
Rapid Identification ◽  
Invasive Group ◽  
Outbreak Investigations ◽  
Group A ◽  
Culture Negative

ABSTRACTWe developed a sequential quadriplex real-time PCR-based method for rapid identification of 20 emm types commonly found in invasive group A Streptococcus (iGAS) strains recovered through the Centers for Disease Control and Prevention’s Active Bacterial Core surveillance. Each emm real-time PCR assay showed high specificity and accurately identified the respective target emm type, including emm subtypes in the United States. Furthermore, this method is useful for rapid typing of GAS isolates and culture-negative specimens during outbreak investigations.

scholarly journals Spectrum and Inoculum Size Effect of a Rapid Antigen Detection Test for Group A Streptococcus in Children with Pharyngitis

PLoS ONE ◽  
2012 ◽  
Vol 7 (6) ◽  
pp. e39085 ◽  
Author(s):  
Jérémie F. Cohen ◽  
Martin Chalumeau ◽  
Corinne Levy ◽  
Philippe Bidet ◽  
Franck Thollot ◽  
...  
Keyword(s):  
Size Effect ◽  
Group A Streptococcus ◽  
Inoculum Size ◽  
Antigen Detection ◽  
Rapid Antigen Detection Test ◽  
Group A

scholarly journals Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests

2000 ◽  
Vol 38 (9) ◽  
pp. 3235-3239 ◽  
Author(s):  
Rashidul Haque ◽  
Nasir Uddin Mollah ◽  
Ibne Karim M. Ali ◽  
Khorshed Alam ◽  
Aleida Eubanks ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Positive Culture ◽  
Antigen Detection ◽  
Pcr Analysis ◽  
Amebic Liver Abscess ◽  
Intestinal Infection ◽  
Entamoeba Dispar ◽  
Stool Specimens ◽  
Culture Negative

A noninvasive diagnostic test for amebic liver abscess is needed, because amebic and bacterial abscesses appear identical on ultrasound or computer tomography and because it is rarely possible to identifyEntamoeba histolytica in stool specimens from patients with amebic liver abscess. Here we report a method of detection in serum of circulating E. histolytica Gal/GalNAc lectin to diagnose amebic liver abscess, which was used in patients from Dhaka, Bangladesh. The TechLab E. histolytica II test (which differentiates the true pathogen E. histolytica fromEntamoeba dispar) detected Gal/GalNAc lectin in the sera of 22 of 23 (96%) amebic liver abscess patients tested prior to treatment with the antiamebic drug metronidazole and 0 of 70 (0%) controls. After 1 week of treatment with metronidazole, 9 of 11 (82%) patients became serum lectin antigen negative. The sensitivity of the E. histolytica II antigen detection test for intestinal infection was also evaluated. Antigen detection identified E. histolytica infection in 50 samples from 1,164 asymptomatic preschool children aged 2 to 5 years, including 16 of 16 (100%) culture-positive specimens. PCR analysis of stool specimens was used to confirm that most antigen-positive but culture-negative specimens were true-positive: PCR identified parasite DNA in 27 of 34 (79%) of the antigen-positive, culture-negative stool specimens. Antigen detection was a more sensitive test for infection than antilectin antibodies, which were detected in only 76 of 98 (78%) amebic liver abscess patients and in 26 of 50 (52%) patients with intestinal infection. We conclude that the TechLab E. histolytica II kit is a sensitive means to diagnose hepatic and intestinal amebiasis prior to the institution of metronidazole treatment.

scholarly journals Evaluation of Rapid Antigen Detection Test for Group A Streptococci Pharyngitis among Children in an Out-Patient Clinic in Malaysia

2021 ◽  
Vol 50 (11) ◽  
pp. 3345-3354
Author(s):  
Zaili Zaki ◽  
Asrul Abdul Wahab ◽  
Ramliza Ramli ◽  
Afaaf Esa ◽  
Ezura Madiana Md. Monoto
Keyword(s):  
Sore Throat ◽  
Sensitivity And Specificity ◽  
Throat Swab ◽  
Group A Streptococcus ◽  
Antigen Detection ◽  
Preschool Age ◽  
Throat Culture ◽  
Care Clinic ◽  
Rapid Antigen Detection Test ◽  
Group A

One of the most common conditions encountered in the out-patient setting is acute pharyngitis. Group A Streptococcus (GAS) accounts for 15%-30% of cases of sore throat particularly in children under 15 years old. Rapid antigen testing (RADT) is an alternative diagnostic method to detect GAS pharyngitis. This study was done to evaluate the agreement between RADT whereby BIONEXIA® Strep A Plus (BioMérieux, France) kit was used and throat culture in the diagnosis of GAS pharyngitis in children presented with a sore throat. One hundred and ten children from a primary health care clinic with sore throat were included in this study. All children were evaluated based on McIsaac scoring and throat swab samples were taken for both throat culture and RADT testing. The prevalence of GAS pharyngitis by RADT in this study was 7.3% over one year. A higher incidence of GAS pharyngitis was noted in the school-aged children than the preschool-age children. There was no correlation between cough, lymph node enlargement, and tonsillar enlargement in predicting GAS pharyngitis. The sensitivity and specificity of RADT were 100% and 98%, respectively, when taking throat culture as a gold standard. A good agreement between RADT and throat culture was achieved (k=0.848). McIsaac scoring was noted to have good predictability for GAS pharyngitis with AUC=0.82. In conclusion, the rapid streptococcal antigen detection test showed excellent sensitivity and specificity and detecting GAS from the throat swab samples. Thus, it can be used to aid in the diagnosis of group A Streptococcal pharyngitis and could reduce the overuse of antibiotics. McIsaac score has also proven to be useful as a screening tool for bacterial pharyngitis.

scholarly journals Modern understanding about the role of group A β -hemolytic streptococcus in acute tonsillitis

2021 ◽  
Vol 13 (4) ◽  
pp. 66-71
Author(s):  
N. T. Mirzoev ◽  
S. N. Sidorchuk ◽  
Yu. I. Bulan’kov ◽  
K. V. Kas’janenko
Keyword(s):  
Predictive Value ◽  
Bacterial Culture ◽  
Detection Method ◽  
Throat Swab ◽  
Diagnostic System ◽  
Antigen Detection ◽  
Acute Tonsillitis ◽  
Hemolytic Streptococcus ◽  
Streptococcal Antigen ◽  
Group A

Objective: assess the modern value of group А β-hemolytic streptococcus in patients with acute tonsillitis and the effectiveness of the rapid streptococcal antigen detection method.Materials and methods: microbial landscape assessment of acute tonsillitis was based on retrospective analysis of 902 bacterial culture results of a throat swab of patients with syndromes of acute tonsillitis treated in the Infectious Diseases Clinic of the Military Medical Academy named after S.M. Kirov during the period of 2019-2020. The effectiveness of the rapid streptococcal antigen detection method in the oropharynx was determined by a prospective study involving 35 patients with acute tonsillitis.Results: in the study, we have found that bacterial culture results of a throat swab, the following were more common: Nesseria species (39 %), Streptococcus viridans (23 %), and Staphylococcus aureus (17 %). The frequency of detection of β-hemolytic streptococcus was 1 %. The rapid diagnostic system «Streptatest» in patients with acute tonsillitis has demonstrated efficiency, under which that sensitivity of test was 80 %, specificity – 90 %, positive predictive value – 57,14 %, negative predictive value – 96,43 %.Conclusions: the frequency of group A β-hemolytic streptococcus in patients with lesion of lymphoid tissues of the oropharynx has declined significantly nowadays. The rapid diagnostic system «Streptatest» is a highly effective medical product that can be used in both hospital and pre-hospital stage. 

scholarly journals A clinical decision rule for streptococcal pharyngitis management: An update

2017 ◽  
Vol 9 (02) ◽  
pp. 116-120 ◽  
Author(s):  
Hosain Nasirian ◽  
Saeedeh TarvijEslami ◽  
Esfandiar Matini ◽  
Seyedehsara Bayesh ◽  
Yasaman Omaraee
Keyword(s):  
Decision Rule ◽  
Positive Culture ◽  
Clinical Decision ◽  
Streptococcal Pharyngitis ◽  
Clinical Findings ◽  
Common Disease ◽  
Clinical Decision Rule ◽  
Throat Culture ◽  
Group A ◽  
Culture Negative

Abstract PURPOSE: Group A streptococcal (GAS) pharyngitis is a common disease worldwide. We aimed to establish a pragmatic program as a clinical decision rule for GAS pharyngitis diagnosis. MATERIALS AND METHODS: This article derived from a research project on children aged 6–15 years. Five hundred and seventy-one children met the enrollment criteria on whom throat culture and validities of clinical findings were assessed in positive and negative throat culture groups. RESULTS: Positive GAS throat culture group included 99 (17.3%) patients with a positive culture. Negative GAS throat culture group included 472 (82.6%) patients. Exudate or enlarged tender nodes each one had 63% and 68% sensitivity and 31.5% and 37.5% specificity with a high percentage of negative predictive value (NPV) 80.54% and 85.09%, respectively. Sequence test revealed validities of exudate plus enlarged nodes at 43.62% sensitivity and 57.19% specificity with 83% NPV. CONCLUSIONS: High NPV of 83% indicated that similar prevalence in the absence of either exudate or enlarged tender lymph nodes. Probability of GAS negative throat cultures among children suspected of GAS pharyngitis was 83% and would correctly not receive inopportune antibiotics.

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