The dual-specificity kinases, TOPK and DYRK1A, are critical for oocyte maturation induced by wild-type-but not by oncogenic- ras-p21 protein

Rat pheochromocytoma (PC12) cells differentiate to neuronal cells in response to nerve growth factor. It has been shown that microinjection of oncogenic but not proto-oncogenic p21 protein induces morphological differentiation in PC12 cells (D. Bar-Sagi and J. R. Feramisco, Cell 42:841-848, 1985). In this paper we describe a recombinant human proto-oncogenic Ha-ras protein which can effectively induce neurite extension of PC12 cells when microinjected as a complex with guanosine-5'-O-(3-thiotriphosphate). The protein was found to be less effective when complexed with GTP. On the other hand, an oncogenic ras protein coinjected with guanosine-5'-O-(2-thiodiphosphate) was entirely inactive. These results indicate that the binary p21-GTP complex, but not the p21-GDP complex, is effective in inducing differentiation in PC12 cells, irrespective of the oncogenic or the proto-oncogenic protein.

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Molecular Biology of GAP and its Interaction with Oncogenic ras p21

ras Oncogenes ◽

10.1007/978-1-4757-1235-3_40 ◽

1989 ◽

pp. 303-309

Author(s):

U. S. Vogel ◽

R. E. Diehl ◽

M. S. Marshall ◽

M. D. Schaber ◽

R. B. Register ◽

...

Keyword(s):

Molecular Biology ◽

Oncogenic Ras ◽

Ras P21

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Induction of neurite formation in PC12 cells by microinjection of proto-oncogenic Ha-ras protein preincubated with guanosine-5'-O-(3-thiotriphosphate).

Molecular and Cellular Biology ◽

10.1128/mcb.7.12.4553 ◽

1987 ◽

Vol 7 (12) ◽

pp. 4553-4556 ◽

Cited By ~ 65

Author(s):

T Satoh ◽

S Nakamura ◽

Y Kaziro

Keyword(s):

Nerve Growth Factor ◽

Pc12 Cells ◽

Morphological Differentiation ◽

The Other ◽

P21 Protein ◽

Ras Protein ◽

Oncogenic Ras ◽

Neurite Formation ◽

Pheochromocytoma Pc12 ◽

Oncogenic Protein

Rat pheochromocytoma (PC12) cells differentiate to neuronal cells in response to nerve growth factor. It has been shown that microinjection of oncogenic but not proto-oncogenic p21 protein induces morphological differentiation in PC12 cells (D. Bar-Sagi and J. R. Feramisco, Cell 42:841-848, 1985). In this paper we describe a recombinant human proto-oncogenic Ha-ras protein which can effectively induce neurite extension of PC12 cells when microinjected as a complex with guanosine-5'-O-(3-thiotriphosphate). The protein was found to be less effective when complexed with GTP. On the other hand, an oncogenic ras protein coinjected with guanosine-5'-O-(2-thiodiphosphate) was entirely inactive. These results indicate that the binary p21-GTP complex, but not the p21-GDP complex, is effective in inducing differentiation in PC12 cells, irrespective of the oncogenic or the proto-oncogenic protein.

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