scholarly journals Case report of goose haemorrhagic polyomavirus in 4-day-old goslings indicating vertical transmissibility

2011 ◽  
Vol 80 (3) ◽  
pp. 255-257 ◽  
Author(s):  
Attila Farsang ◽  
Sandor Bernath ◽  
Mihaly Dobos-Kovacs
Keyword(s):  
Case Report ◽  
Disease Control ◽  
Real Time ◽  
Incubation Period ◽  
Vertical Transmission ◽  
Real Time Pcr ◽  
Viral Transmission ◽  
Pcr Assay ◽  
Fatal Disease ◽  
First Time

Haemorrhagic nephritis and enteritis of geese (HNEG) is a fatal disease caused by goose haemorrhagic polyomavirus (GHPV). The aim of our study was to investigate a field outbreak of HNEG by pathological methods and real-time PCR assay using light upon extension (LUX PCR) with special regard to the possibility of vertical transmission. This is the first time that presence of GHPV was confirmed in goslings that died within 4 days after hatching showing typical symptoms of HNEG, which indicates vertical transmissibility as the shortest incubation period of HNEG is 6 days. The way of viral transmission is a key issue and thus the disease control measurements and HNEG epizootiology may be revised based on the findings of this study.

scholarly journals Transplacental Transmission Of SARS-Cov-2 Infection: A Case Report from Iran

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Farhad Abolhasan Choobdar ◽  
Maral Ghassemzadeh ◽  
Mohammad Attarian ◽  
Ezzat Abbariki ◽  
Alireza Nateghian ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Case Report ◽  
Viral Load ◽  
Real Time ◽  
Vertical Transmission ◽  
Real Time Pcr ◽  
Myeloid Leukemia ◽  
Transplacental Transmission ◽  
Acute Myeloid

: We report a case of SARS-CoV-2 vertical transmission through the placenta in a neonate whose mother had non-M3 acute myeloid leukemia (AML) that was complicated with Covid-19 in the last trimester. Viral load in nasopharyngeal swabs from mother and neonate were high. Real-time PCR of the fetal side of the placenta was positive for SARS-CoV-2, which makes it possible to consider this case as a congenital case of SARS-CoV-2 infection that is transmitted through vertical transmission.

scholarly journals A misleading false-negative result of Pneumocystis real-time PCR assay due to a rare punctual mutation: A French multicenter study

2016 ◽  
Vol 55 (2) ◽  
pp. 180-184 ◽  
Author(s):  
Solène Le Gal ◽  
Florence Robert-Gangneux ◽  
Yann Pépino ◽  
Sorya Belaz ◽  
Céline Damiani ◽  
...  
Keyword(s):  
Real Time ◽  
Multicenter Study ◽  
Real Time Pcr ◽  
False Negative ◽  
False Negative Result ◽  
Pcr Assay

scholarly journals Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 188
Author(s):  
Tanja Hoffmann ◽  
Andreas Hahn ◽  
Jaco J. Verweij ◽  
Gérard Leboulle ◽  
Olfert Landt ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Pcr Assay ◽  
Bead Beating ◽  
Stool Samples ◽  
Pcr Assays ◽  
Human Stool

This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as well as proteinase K digestion were compared with group-, genus-, and species-specific real-time PCR assays targeting helminths and nonhelminth pathogens in human stool samples. From 25 different in-house and commercial helminth real-time PCR assays applied to 77 stool samples comprising 67 historic samples and 10 external quality assessment scheme samples positively tested for helminths, higher numbers of positive test results were observed after bead-beating-based nucleic acid extraction for 5/25 (20%) real-time PCR assays irrespective of specificity issues. Lower cycle threshold values were observed for one real-time PCR assay after the standard extraction scheme, and for four assays after the bead-beating-based scheme. Agreement between real-time PCR results after both nucleic acid extraction strategies according to Cohen’s kappa ranged from poor to almost perfect for the different assays. Varying agreement was observed in eight nonhelminth real-time PCR assays applied to 67 historic stool samples. The study indicates highly variable effects of harsh nucleic acid extraction approaches depending on the real-time PCR assay used.

Development and application of a TaqMan-based real-time PCR assay for specifically detecting feline astrovirus

2021 ◽  
pp. 101729
Author(s):  
Guoying Dong ◽  
Qian Wang ◽  
Jiangting Niu ◽  
Yiqin Cai ◽  
Yanbing Guo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay
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