scholarly journals Diagnosis of the bovine leukaemia virus infection in Polish Holstein-Friesian cows and comparison of their milk productivity

2012 ◽  
Vol 81 (4) ◽  
pp. 353-358 ◽  
Author(s):  
Małgorzata Szewczuk ◽  
Sławomir Zych ◽  
Sylwia Katafiasz
Keyword(s):  
Virus Infection ◽  
Multiplex Pcr ◽  
Milk Yield ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serological Response ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
Holstein Friesian

Economic losses due to the bovine leukaemia virus infection can come from reduced milk production. The aim of this study was to evaluate molecular multiplex Polymerase Chain Reaction (multiplex PCR) test as an alternative for serologic Enzyme-linked Immunosorbent Assay (ELISA) method in diagnosing bovine leukaemia virus infections and found possible differences in milk yield in seronegative and seropositive cows. The study involved two groups of Polish Holstein-Friesian var. black and white cows (a total of 147 individuals). Animals were grouped according to their serological response to the bovine leukaemia virus antigen: control group of 71 healthy cows and second group of 76 naturally infected animals. Both multiplex PCR and ELISA proved to be very sensitive methods with sensitivity up to 100% and 97.4%, respectively. Negative effect of bovine leukaemia virus on milk yield was observed. Leucosis-free cows achieved higher milk yield (+ 322.9 kg;P≤ 0.01), protein yield (+ 5.2 kg,P≤ 0.05), and better milk yield after calculation into daily milk yield corrected for fat (+ 106.6 kg,P≤ 0.05) in comparison to seropositive cows. In infected cows a significantly higher (P≤ 0.01) fat content (4.1%) was recorded. Because ELISA test does not provide information of the infection at an early stage and is not sensitive enough to detect every infected animal, a two-step protocol allows for elimination of seropositive animals and restriction of their introduction into herds in Europe.

Preliminary Studies on Impact of Bovine Leukaemia Virus Infection on Dairy Productivity

1982 ◽  
pp. 599-605 ◽  
Author(s):  
M. J. Burridge ◽  
M. C. Thurmond ◽  
D. M. Puhr ◽  
C. J. Wilcox ◽  
N. A. Simerl
Keyword(s):  
Virus Infection ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus

Establishment of a novel diagnostic test for Bovine leukaemia virus infection using direct filter PCR

2020 ◽  
Vol 67 (4) ◽  
pp. 1671-1676
Author(s):  
Hala El Daous ◽  
Shuya Mitoma ◽  
Eslam Elhanafy ◽  
Huyen Thi Nguyen ◽  
Ngan Thi Mai ◽  
...  
Keyword(s):  
Virus Infection ◽  
Diagnostic Test ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus

An enzyme-linked immunosorbent assay for detection of bovine leukaemia virus p24 antibody in cattle

1990 ◽  
Vol 28 (1) ◽  
pp. 47-57 ◽  
Author(s):  
John B. Molloy ◽  
Peter J. Walker ◽  
F.Chris Baldock ◽  
Barry J. Rodwell ◽  
Jeff A. Cowley
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus

scholarly journals Evaluation of Cross Protective Efficacy of Commercial Vaccines against Mannheimia haemolytica in Mice

2019 ◽  
Vol 43 (1) ◽  
pp. 165-170
Author(s):  
Waffa A. Ahmed
Keyword(s):  
Immunosorbent Assay ◽  
Protective Efficacy ◽  
Challenge Test ◽  
Cross Protection ◽  
Mannheimia Haemolytica ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Significant Difference ◽  
Protection Efficacy

Mannheimia haemolytica together with Pasteurella multocida represents as a major bacterial causative agent of cattle, sheep and goats respiratory diseases and its one of the most important causes for economic losses to these animals .Commercially available vaccines were used to prevent infections caused by P. multocida and M. haemolytica. Thus, the aim of the present study was to evaluate the cross protection efficacy of two vaccines to protect mice against M.haemolytica, studying humeral immunity, using Enzyme-Linked Immunosorbent Assay. Forty five mice were divided into three equal groups, group one and two were inoculated subcutaneously  4μl\JOVAPAST® and 1μl of Al-kindy vaccines respectively, while the third group was with 0.5 ml sub cutaneous PBS. LD50for M.haemolytica was estimated as 2× 106 cfu \ml and challenge test was conducted by dropping 0.05 ml 2× 106 cfu \ml intranasally after three weeks of immunization for the three groups. The results of Enzyme-Linked Immunosorbent Assay, showed significant increase of antibody titters at (P<0.01) in (group 1 and 2) after first and second weeks post immunization, in comparison with control group. Also, the re-isolation of M.haemolytica from lungs tissue of all groups after challenged were positive with significant difference between control and immunized group, control group was 4× 108 cfu ∕ml which was higher than immunized group one and group two,which were 2.5×104 cfu∕ml and 3,5×105 cfu∕ml respectively after 24 hour of vaccine. In conclusion, the two commercial vaccines showed good cross protection efficacy against M. haemolytica, but JOVAPAST® vaccine showed higher efficacy than Alkindy vaccine, as that it contain  two  heterologous  killed strains and providing the basis for production a vaccine from the two  pathogen of local strains. 

scholarly journals Bovine Leukaemia Virus: Current Epidemiological Circumstance and Future Prospective

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2167
Author(s):  
Marawan A. Marawan ◽  
Abdulaziz Alouffi ◽  
Suleiman El Tokhy ◽  
Sara Badawy ◽  
Ihsanullah Shirani ◽  
...  
Keyword(s):  
Effective Control ◽  
Cell Leukaemia ◽  
Economic Losses ◽  
Effective Vaccine ◽  
Neoplastic Disease ◽  
Persistent Lymphocytosis ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
Human T Cell ◽  
Bovine Leukosis

Bovine leukaemia virus (BLV) is a deltaretrovirus that is closely related to human T-cell leukaemia virus types 1 and 2 (HTLV-1 and -2). It causes enzootic bovine leukosis (EBL), which is the most important neoplastic disease in cattle. Most BLV-infected cattle are asymptomatic, which potentiates extremely high shedding rates of the virus in many cattle populations. Approximately 30% of them show persistent lymphocytosis that has various clinical outcomes; only a small proportion of animals (less than 5%) exhibit signs of EBL. BLV causes major economic losses in the cattle industry, especially in dairy farms. Direct costs are due to a decrease in animal productivity and in cow longevity; indirect costs are caused by restrictions that are placed on the import of animals and animal products from infected areas. Most European regions have implemented an efficient eradication programme, yet BLV prevalence remains high worldwide. Control of the disease is not feasible because there is no effective vaccine against it. Therefore, detection and early diagnosis of the disease are essential in order to diminish its spreading and the economic losses it causes. This review comprises an overview of bovine leukosis, which highlights the epidemiology of the disease, diagnostic tests that are used and effective control strategies.

scholarly journals Baculovirus expression and potential diagnostic application of the gp51 envelope glycoprotein of genetic mutants of the bovine leukaemia virus

2019 ◽  
Vol 63 (1) ◽  
pp. 1-6
Author(s):  
Marzena Rola-Łuszczak ◽  
Agnieszka Grabowska ◽  
Bogusław Szewczyk ◽  
Jacek Kuźmak
Keyword(s):  
Envelope Glycoprotein ◽  
Insect Cells ◽  
3D Structure ◽  
Recombinant Baculovirus ◽  
Surface Glycoprotein ◽  
Serological Response ◽  
Bovine Leukaemia Virus ◽  
Leukaemia Virus ◽  
Baculovirus Expression ◽  
Genetic Mutants

AbstractIntroduction:Field isolates of bovine leukaemia virus (BLV) show the presence of a few amino acid substitutions in major conformational G and H epitopes on surface glycoprotein gp51. Potentially, these substitutions can affect the 3D structure of these epitopes leading to their diminished immunoreactivity. The aim of this study was to express three gp51 glycoproteins carrying mutated epitopes as recombinant baculovirus proteins in insect cells to test their immunoreactivity with bovine sera.Material and Methods:Envgene chimeras encoding mutated epitopes G and H in theenvbackbone of BLV FLK strain were constructed, cloned into pFastBac1 vector, and expressed in baculovirus.Results:The presence of recombinant gp51 protein in Sf9 insect cells was confirmed using monoclonal antibodies. ELISA tests were developed to check the immunoreactivity of recombinant protein with bovine sera.Conclusion:Recombinant gp51 proteins with altered G and H epitopes can be used for further studies to analyse the serological response of bovine sera towards BLV antigenic variants.

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