2004 ◽  
Vol 22 (1) ◽  
pp. 19-24 ◽  
Author(s):  
F. PEGORARO ◽  
S. ATZENI ◽  
M. BORGHESI ◽  
S. BULANOV ◽  
T. ESIRKEPOV ◽  
...  

Energetic ion beams are produced during the interaction of ultrahigh-intensity, short laser pulses with plasmas. These laser-produced ion beams have important applications ranging from the fast ignition of thermonuclear targets to proton imaging, deep proton lithography, medical physics, and injectors for conventional accelerators. Although the basic physical mechanisms of ion beam generation in the plasma produced by the laser pulse interaction with the target are common to all these applications, each application requires a specific optimization of the ion beam properties, that is, an appropriate choice of the target design and of the laser pulse intensity, shape, and duration.


Author(s):  
Łukasz Piotr Baran ◽  
Wojciech Rżysko ◽  
Dariusz Tarasewicz

In this study we have performed extensive coarse-grained molecular dynamics simulations of the self-assembly of tetra-substituted molecules. We have found that such molecules are able to form a variety of...


Author(s):  
Miguel A. Losada ◽  
M. Izaskun Benedicto
Keyword(s):  

2021 ◽  
Author(s):  
◽  
Oliver Wright

<p>This research investigates a progression away from acoustics formed by spaces and towards spaces that are driven by target acoustics. Despite architecture and acoustics’ shared consideration of form, materiality and inhabitation, too often acoustics is neglected from design and so is treated remedially, nullifying creativity. A case study project was undertaken to investigate the opportunities and limitations of two parametric tools, Galapagos (a generative solver) and Pachyderm (an acoustic simulation tool), to develop acoustic qualities in early architectural design. Yet, what are these acoustic qualities and how could they be measured? Testing of cafes in the Wellington CBD was undertaken to investigate these questions.  Six cafes were acoustically tested and five patrons from each of these completed a subjective survey. The café testing suggested that Reverberation Time (RT) could be an effective acoustic measure to direct architectural design. The café with the lowest patron enjoyment rating also recorded the longest RT and highest Sound Pressure Level (SPL), reinforcing the relationship between these three elements. Through these findings, patron enjoyment was concluded to be dependent on SPL and SPL was concluded to be dependent on RT (Whitlock and Dodd, 424). In order to increase patron enjoyment, Galapagos was utilised to explore possible forms that met a target design RT of 0.7 seconds. An RT of 0.7 seconds was chosen as it was shorter than the AS/NZS 2107 (2000) maximum and was comparable to the cafés with the two highest subjective enjoyment ratings.  Through a parametric and analogue design methodology, Galapagos and Pachyderm were used to investigate how acoustic goals could shape a café design. The case study project produced a design that not only meets this acoustic criterion but harnesses form to sculpt sound. Instead of applying absorption to flat surfaces, the convex curves on the north and east facades disperse sound, producing both a diffuse environment and an engaging architectural element. This integrated investigation demonstrated that a parametric and analogue design process can be implemented to create a acoustically and architecturally effective design.</p>


2019 ◽  
Author(s):  
Kendall R Sanson ◽  
Peter C DeWeirdt ◽  
Annabel K Sangree ◽  
Ruth E Hanna ◽  
Mudra Hegde ◽  
...  

ABSTRACTCas12a enzymes have attractive properties for scalable delivery of multiplexed perturbations, yet widespread usage has lagged behind Cas9-based strategies. Here we describe the optimization of Cas12a from Acidaminococcus (AsCas12a) for use in pooled genetic screens in human cells. By assaying the activity of thousands of guides, we confirm on-target design rules and extend them to an enhanced activity variant, enAsCas12a. We also develop the first comprehensive set of off-target rules for Cas12a, and demonstrate that we can predict and exclude promiscuous guides. Finally, to enable efficient higher-order multiplexing via lentiviral delivery, we screen thousands of direct repeat variants and identify 38 that outperform the wildtype sequence. We validate this optimized AsCas12a toolkit by targeting 12 synthetic lethal gene pairs with up to 400 guide pairs each, and demonstrate effective triple knockout via flow cytometry. These results establish AsCas12a as a robust system for combinatorial applications of CRISPR technology.


2019 ◽  
Author(s):  
Forrest Robert Svingala ◽  
Mario Valles Montenegro
Keyword(s):  

2017 ◽  
Vol 36 (2-3) ◽  
pp. 80-86
Author(s):  
S. A. Ghorashi ◽  
M. Mahdavi
Keyword(s):  

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