scholarly journals Composição do meio de cultivo para produção de microplantas de caju-de-árvore-do-Cerrado (Anacardium othonianum RIZZ.) - Composition of the cultivation medium for the production of microplants of Cerrado-tree cashew (Anacardium othonianum RIZZ.)

2017 ◽  
Vol 4 (1) ◽  
pp. 01 ◽  
Author(s):  
Ísis Danielle Sousa ◽  
Jackellyne Bruna Sousa ◽  
Flávia Dionísio Pereira ◽  
João Das Graças Santana ◽  
Aurélio Rubio Neto ◽  
...  
Keyword(s):  
Activated Charcoal ◽  
Large Scale ◽  
Culture Medium ◽  
Average Length ◽  
Scale Production ◽  
Cultivation Medium ◽  
Large Scale Production ◽  
Number Of Leaves ◽  
Short Time

RESUMOO Anacardium othonianum Rizz. é uma planta típica de regiões de clima tropical caracterizada pela aparência exótica e aroma agradável. Na busca de diversificar a produção e atividades que proporcione maior rentabilidade, a micropropagação tem sido uma alternativa para produção em grande escala em curto espaço de tempo. Por isso, objetivou-se com este trabalho determinar as melhores condições in vitro para micropropagação dessa espécie, para isso, avaliamos a adição de diferentes concentrações de AIB (Ácido Indolbutírico), sacarose e carvão ativado no meio de cultivo in vitro. Foram utilizadas cinco concentrações de AIB (0; 1; 2; 3; 4 mg L-1) e cinco concentrações de sacarose (0, 15, 30, 45, 60 g L-1) na ausência ou presença de carvão ativado (2 g L-1), em meio WPM 50%. Aos 30 e 60 dias foram feitas avaliações do número de explantes oxidados, comprimento médio e número de folhas por explante. Verificou-se que o meio de cultivo suplementado com carvão ativado e a adição de 4 mg L-1 de AIB, contribuiu para a o crescimento de raízes in vitro da espécie. Enquanto, que o meio de cultivo com 30 g sacarose e presença de carvão ativado proporcionou maior comprimento dos explantes e maior número de folhas.Palavras-chave: Frutífera nativa, cerrado, micropropagação.ABSTRACTThe Anacardium othonianum Rizz. It’s a typical plant of regions of tropical climate characterized by the exotic appearance and pleasant aroma. In the quest to diversify production and activities that provide greatest profitability, micropropagation has been an alternative for large-scale production in short time. Therefore, the objective of this work is to determine the best in vitro results for micropropagation of this species, for this, we evaluated the addition of different concentrations of AIB (Indolbutyric Acid), sucrose and activated charcoal in the in vitro culture medium. Five concentrations of AIB (0, 1, 2, 3, 4 mg L-1) and five sucrose concentrations (0, 15, 30, 45, 60 g L-1) were used in the absence or presence of activated charcoal, in WPM 50% medium. At 30 and 60 days, the number of oxidation, average length and number of leaves per explant were evaluated. It was found that the culture medium supplemented with activated charcoal and an addition of 4 mg L-1 of IBA, contributed to in vitro root growth. While the culture medium with 30 g L-1 sucrose and the presence of activated charcoal provided a longer length of the explants and a larger number of leaves.Key words: Native Fruit, Cerrado, Micropropagation.

scholarly journals Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Revista CERES ◽  
2013 ◽  
Vol 60 (2) ◽  
pp. 152-160 ◽  
Author(s):  
Leticia Mascarenhas Pereira Barbosa ◽  
Vespasiano Borges de Paiva Neto ◽  
Leonardo Lucas Carnevalli Dias ◽  
Reginaldo Alves Festucci-Buselli ◽  
Rodrigo Sobreira Alexandre ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Large Scale ◽  
Culture Medium ◽  
Shoot Proliferation ◽  
Cellular Level ◽  
Fragaria X Ananassa ◽  
Scale Production ◽  
Ms Medium ◽  
Large Scale Production

In vitro propagation has become an effective practice for large-scale production of strawberry plants. The objective of this study was to evaluate the hyperhydricity and the multiplication capacity of two strawberry varieties (Fragaria x ananassa Duch. 'Dover' and 'Burkley') propagated in vitro. Plants maintained in MS medium supplemented with 1.0 mg L-1 BA were individualized and transferred to the same medium solidified with Agar (6.5 g L-1) or Phytagel® (2.5 g L-1) and BA at different concentrations (0; 0.5; 1.0; 2.0 and 3.0 mg L-1). Biochemical and anatomical analyses were carried out, as well as the analysis of the morphological hyperhydricity characteristics. The analysis of data showed: a) the increase in cytokinin concentration increased hyperhydricity frequency in both varieties; b) at concentrations up to 2.0 mg L-1 BA, the replacement of Agar by Phytagel® induced a higher formation of hyperhydric shoots; and c) the addition of BA induced oxidative stress, which is characterized by increased antioxidant activity and lipid peroxidation, as well as alterations at the cellular level, such as malformation of stomata and epidermal cells. In conclusion, the culture medium containing 0.5 mg L-1 BA solidified with Agar provided lower hyperhydricity percentages in association with higher rates of shoot proliferation in strawberry.

scholarly journals A Review on Micropropagation Culture Method

2020 ◽  
Vol 8 (1) ◽  
pp. 86-93 ◽  
Author(s):  
Nikita Gupta ◽  
Vidhi Jain ◽  
Merin Rosy Joseph ◽  
Siwani Devi
Keyword(s):  
Large Scale ◽  
Low Cost ◽  
Culture Method ◽  
Meristem Culture ◽  
Culture Vessel ◽  
Scale Production ◽  
Large Scale Production ◽  
Aseptic Conditions ◽  
Short Time

Micropropagation is a vegetative propagation conducted under controlled and aseptic conditions in the microenvironment of the culture vessel, which have the all growth requirements of a plant in the natural conditions. Recently different techniques of propagation have been developed which could facilitate large scale production of plants and for the improvement of the species. An overview on the in vitro propagation via meristem culture, callus culture and protoplast culture etc. are presented here. Today micropropagation techniques are applied in order to produce large numbers of new high-quality plants in a relatively short time and space, in low cost and can also be preserved.  

Optimizing in vitro large scale production of giant reed (Arundo donax L.) by liquid medium culture

2014 ◽  
Vol 69 ◽  
pp. 21-27 ◽  
Author(s):  
Valeria Cavallaro ◽  
Cristina Patanè ◽  
Salvatore L. Cosentino ◽  
Isabella Di Silvestro ◽  
Venera Copani
Keyword(s):  
Liquid Medium ◽  
Large Scale ◽  
Arundo Donax ◽  
Giant Reed ◽  
Scale Production ◽  
Large Scale Production ◽  
Medium Culture ◽  
Arundo Donax L

Large-scale production of polyoma middle T antigen by using genetically engineered tumors

1985 ◽  
Vol 5 (7) ◽  
pp. 1795-1799
Author(s):  
D R Kaplan ◽  
B Bockus ◽  
T M Roberts ◽  
J Bolen ◽  
M Israel ◽  
...  
Keyword(s):  
Nude Mice ◽  
Large Scale ◽  
T Antigen ◽  
Genetically Engineered ◽  
Scale Production ◽  
Large Scale Production ◽  
Metallothionein Promoter ◽  
Polyoma Middle T Antigen ◽  
Nih 3T3

A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 to 1,000 100-mm dishes of polyomavirus-infected cells. This middle T, partially purified by immunoaffinity chromatography, retained activity as measured by its ability to be phosphorylated in vitro. The combined approach of fusing strong promoters to genes of interest and utilizing nude mice to grow large quantities of cells expressing the gene provides a quick, inexpensive alternative to other expression systems.

scholarly journals Production of Phytotoxic Metabolite Using Biphasic Fermentation System from Strain C1136 of Lasiodiplodia pseudotheobromae, a Potential Bioherbicidal Agent

2017 ◽  
Vol 9 (3) ◽  
pp. 371-377
Author(s):  
Charles Oluwaseun ADETUNJI ◽  
Julius Kola OLOKE ◽  
Gandham PRASAD ◽  
Moses ABALAKA ◽  
Emenike Onyebum IROKANULO
Keyword(s):  
Large Scale ◽  
Wild Strain ◽  
Fermentation Medium ◽  
Scale Production ◽  
Conventional Farming ◽  
Large Scale Production ◽  
Phytotoxic Metabolite ◽  
Biphasic Fermentation

Formulation of effective and environmental friendly bioherbicides depends on the type of fermentation medium used for the production of phytotoxic metabolites. The effect of biomass, colony forming unit and the phytotoxic metabolite produced from the biphasic fermentation was carried out, while the phytotoxic metabolite was  tested in vivo and in-vitro on Echinochola crus-galli and dicotyledonous Chromolaena odorata. The mutant strain of Lasiodiplodia pseudotheobromae C1136 (Lp90) produced the highest amount of conidia and the largest necrotic area on the two tested weeds when compared to its wild strain in the different biphasic media combinations. The study revealed that the biphasic system containing PDB + rice produced the highest bioherbicidal activities. Therefore, the phytotoxic metabolites from strain C1136 are suggested for large scale production of bioherbicides for the management of weeds in conventional farming to improve yield and enhance food security.

scholarly journals Expansion of Human Pluripotent Stem Cell-derived Early Cardiovascular Progenitor Cells by a Cocktail of Signaling Factors

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Sadaf Vahdat ◽  
Sara Pahlavan ◽  
Elena Mahmoudi ◽  
Maryam Barekat ◽  
Hassan Ansari ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Large Scale ◽  
Culture Medium ◽  
Gene Expression Pattern ◽  
Extended Period ◽  
Scale Production ◽  
Chemical Screening ◽  
Wide Range ◽  
Cell Sources

Abstract Cardiovascular progenitor cells (CPCs) derived from human pluripotent stem cells (hPSCs) are proposed to be invaluable cell sources for experimental and clinical studies. This wide range of applications necessitates large-scale production of CPCs in an in vitro culture system, which enables both expansion and maintenance of these cells. In this study, we aimed to develop a defined and efficient culture medium that uses signaling factors for large-scale expansion of early CPCs, called cardiogenic mesodermal cells (CMCs), which were derived from hPSCs. Chemical screening resulted in a medium that contained a reproducible combination of three factors (A83-01, bFGF, and CHIR99021) that generated 1014 CMCs after 10 passages without the propensity for tumorigenicity. Expanded CMCs retained their gene expression pattern, chromosomal stability, and differentiation tendency through several passages and showed both the safety and possible cardio-protective potentials when transplanted into the infarcted rat myocardium. These CMCs were efficiently cryopreserved for an extended period of time. This culture medium could be used for both adherent and suspension culture conditions, for which the latter is required for large-scale CMC production. Taken together, hPSC-derived CMCs exhibited self-renewal capacity in our simple, reproducible, and defined medium. These cells might ultimately be potential, promising cell sources for cardiovascular studies.

scholarly journals Large-scale production of polyoma middle T antigen by using genetically engineered tumors.

1985 ◽  
Vol 5 (7) ◽  
pp. 1795-1799 ◽  
Author(s):  
D R Kaplan ◽  
B Bockus ◽  
T M Roberts ◽  
J Bolen ◽  
M Israel ◽  
...  
Keyword(s):  
Nude Mice ◽  
Large Scale ◽  
T Antigen ◽  
Genetically Engineered ◽  
Scale Production ◽  
Large Scale Production ◽  
Metallothionein Promoter ◽  
Polyoma Middle T Antigen ◽  
Nih 3T3

A recombinant plasmid containing a metallothionein promoter-polyoma middle T cDNA fusion was constructed and used to transfect NIH 3T3 cells. Transformed cells expressing middle T were injected into nude mice. Within 3 weeks, each mouse produced tumors containing middle T equivalent to that in 250 to 1,000 100-mm dishes of polyomavirus-infected cells. This middle T, partially purified by immunoaffinity chromatography, retained activity as measured by its ability to be phosphorylated in vitro. The combined approach of fusing strong promoters to genes of interest and utilizing nude mice to grow large quantities of cells expressing the gene provides a quick, inexpensive alternative to other expression systems.

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