scholarly journals STUDY ON PATHOGENICITY OF PASTEURELLA MULTOCIDA WHICH ISOLATED FROM FARM ANIMALS AND MAN

2021 ◽  
Vol 26 (1) ◽  
pp. 74-81
Author(s):  
S. S. A. Mobarak ◽  
A. K. Shubber ◽  
A. S. Raheem
Keyword(s):  
Pasteurella Multocida ◽  
Culture Media ◽  
Agar Medium ◽  
Farm Animals ◽  
Pathogenicity Test ◽  
Infected Farm ◽  
Human Samples ◽  
Selective Agar ◽  
Suppurative Pneumonia ◽  
Animal Isolates

This study was described for the nature of the pathpgenesis of bacteria Pasteurella multocida which was isolated from infected man made comparison between these bacteria and those from infected farm animals. The percentage of Pasteurella multcida diagnosed bacteria from animals and human was 29.4% and 16.9% respectively. Comparing to other culture media Pasteurella multocida selective agar medium was characterized by its selectivity and sensitivity and then was attempt for biotyping species and subspecies of isolated Pasteurella from animals and human samples were successfully achieved. Pathogenicity test was performed on mice, only nine human isolatetes and twenty-one animal isolates from Pasteurella multocida were virulent. Todistinguish between the pathogenesis of human and animal isolates, one isolated from human and animal were chosed, in addition to the standared strain. The mice had been experimentally infected by three different ways, I/P, I/T, I/Eye. The results were showed that Pasteurella multocida can produce lesions as fibrinous suppurative pneumonia in lungs, liver and spleen which were detected histopatho logically. However the animal isolates were more virulent than human or standared strain.

scholarly journals Study on the isolation and identification of Pasteurella species from farm animal and human

2008 ◽  
Vol 2 (1) ◽  
pp. 81-90
Author(s):  
Sawsan salman Atia mubarak ◽  
Ismail khatham Shubar ◽  
Amar salim Rahiem
Keyword(s):  
Pasteurella Multocida ◽  
Agar Medium ◽  
Brain Heart Infusion ◽  
Farm Animal ◽  
Brain Heart Infusion Broth ◽  
Laboratory Animals ◽  
Isolation And Identification ◽  
Selective Agar

This study was interested in isolation and identification of Pasteuralla species in animals and human , also studing it's sensitivity to antibiotic with comparison to it's pathogenicity for laboratory animals . Tow groups of samples were collected and investigeted, 1st group consisting of one handered thirty six samples which were collected from animals ( cow .sheep , goats , and chikens ) . 2nd group was also consisting of 136 samples that were colleeted from human ( wound , urine , and sputum ) . These samples were cultured in selective enrichement brain heart infusion broth and then in the new Pasteurella multocida selective agar medium .

scholarly journals Xylose-Lysine-Tergitol 4: An Improved Selective Agar Medium for the Isolation of Salmonella

1991 ◽  
Vol 70 (12) ◽  
pp. 2429-2432 ◽  
Author(s):  
RUSSELL G. MILLER ◽  
C.R. TATE ◽  
E.T. MALLINSON ◽  
J.A. SCHERRER
Keyword(s):  
Agar Medium ◽  
Selective Agar

Assessment of different selective agar media for enumeration and isolation ofListeriafrom dairy products

1988 ◽  
Vol 55 (4) ◽  
pp. 579-583 ◽  
Author(s):  
Lucas Dominguez ◽  
José Francisco Fernández ◽  
Victor Briones ◽  
José Luis Blanco ◽  
Guillermo Suárez
Keyword(s):  
Dairy Products ◽  
Agar Medium ◽  
Raw Milk ◽  
Superior Performance ◽  
Direct Plating ◽  
Selective Agar ◽  
Natural Microflora ◽  
Agar Media ◽  
The Difference ◽  
Better Than

SummaryDifferent selective agar media were compared for the recovery and isolation of five species ofListeriafrom raw milk and cheese. The selective media examined were Beerens medium, MacBride medium and that described by Dominguezet al.(1984) with 6 mg/1 acriflavine, listeria selective agar medium (LSAM), and LSAM with 12 mg/1 acriflavine (LSAM × 2A); a non-selective yeast glucose Lemco agar was included for comparison. When the difference between listeria and the natural microflora of raw milk and cheese was 102cfu/ml, listeria could be isolated by direct plating on all media tested. When it was lower than 103–104cfu/ml, listeria were isolated by direct plating only on LSAM and LSAM × 2A. When the difference was greater than 104cfu/ml, a previous enrichment was necessary to isolate them. LSAM and LSAM × 2A media performed better than the other media tested for isolating listeria by direct plating and improved their isolation from dairy products. This superior performance was evaluated by the ability of these media to support colony formation of different species ofListeriatested, the easy recognition of these colonies from those formed by other microorganisms and by their capacity to inhibit the natural microflora of these foods.

scholarly journals Growth and sporulation of Metarhizium flavoviride var. Flavoviride on culture media and lighting regimes

2001 ◽  
Vol 58 (3) ◽  
pp. 613-616 ◽  
Author(s):  
Sideney Becker Onofre ◽  
Cindia Mara Miniuk ◽  
Neiva Monteiro de Barros ◽  
João Lúcio Azevedo
Keyword(s):  
Biological Control ◽  
Mycelial Growth ◽  
Culture Media ◽  
Agar Medium ◽  
Potato Dextrose Agar ◽  
Continuous Illumination ◽  
Agricultural Pests ◽  
Dark Cycle ◽  
Metarhizium Flavoviride ◽  
Conidia Production

Entomopathogenic fungi from the genus Metarhizium are largely used for the biological control of agricultural pests by conidia spreading on the field. Although conidia production is well studied in M. anisopliae, only few research studies were done in M. flavoviride. The present work was carried out alming to evaluate the Mycelial growth and sporulation of the entomopathogenic fungus Metarhizium flavoviride var. flavoviride growing at 27 ± 2°C on Potato-dextrose-agar (PDA), Czapek-agar (CZP) and a complete agar medium (CM) under three lighting regimes, (continuous illumination, light/dark cycle and an black light/dark cycle) were investigated. A completely randomized 3 × 3 (culture media × lighting regime) factorial design with four replicates was used. The best mycelial growth and sporulation occurred on the PDA and CM media under continuous illumination (P <= 0,05).

scholarly journals The effect of calcium antagonists on the growth of Entamoeba histolytica in vitro

2010 ◽  
Vol 4 (1) ◽  
pp. 5-10
Author(s):  
Zahra’a Abdul-Raheem Ahmed ◽  
Ali H. Ad’hiah ◽  
Amna N. Jasim
Keyword(s):  
Entamoeba Histolytica ◽  
Calcium Antagonists ◽  
Culture Media ◽  
Agar Medium ◽  
Reproduction Rate ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Infusion Agar ◽  
Ethylene Diaminetetraacetic Acid

he E. histolytica parasite was maintained in vitro using Locke-egg medium (LEM) and Liver infusion agar medium (LIAM). The effect of two calcium antagonists (Nifedipine and Ethylene-diaminetetraacetic acid EDTA) on the growth and activity of the parasite in the two culture media was investigated. The calcium antagonists Nifedipine and EDTA inhibited the reproduction rate of E. histolytica in a concentration-dependent manner. For Nifedipine, a concentration of 41.6 mg/ml inhibited the reproduction rate to 99.7% in both media. The EDTA had an approximate effect (98.2 and 95.8)% at a concentration of 0.83 mg/ml in LEM and LIAM media, respectively. Additionally, some cases of a parasite encystment were observed in LEM medium that was treated with Nifedipine.

Procedures for Recovery of Stressed and Injured Cells of Yersinia Enterocolitica from Meat and Meat Products

1989 ◽  
Vol 52 (5) ◽  
pp. 360-362 ◽  
Author(s):  
ZHEKO KOUNEV
Keyword(s):  
Yersinia Enterocolitica ◽  
Agar Medium ◽  
Alkali Treatment ◽  
Meat Products ◽  
The Other ◽  
Step Procedure ◽  
Selective Agar ◽  
Agar Media ◽  
Phosphate Buffered Saline

A new three-step procedure (TSP) for the recovery of Yersinia enterocolitica 0:3 from frozen meat, salted and dried meat products, raw dried meat products, and cooked perishable sausages, has been developed. The TSP is based mainly on enrichment in 0.15 M phosphate-buffered saline at 25°C. In the TSP, selected dilutions of samples are enriched for 1, 2, 3, 4, 24, and 48 h and then plated onto nonselective and selective agar media after or without alkali treatment. Additional enrichment was performed with half of the samples at 25°C for 24 h, and the rest at 4°C for up to 2 wk, followed by alkali treatment and plating on selective agar medium. Recovery of Y. enterocolitica was better using the TSP than the other method used for isolating the organism from meats.

scholarly journals Prevalence and Distribution of Listeria monocytogenesinlAAlleles Prone to Phase Variation andinlAAlleles with Premature Stop Codon Mutations among Human, Food, Animal, and Environmental Isolates

2015 ◽  
Vol 81 (24) ◽  
pp. 8339-8345 ◽  
Author(s):  
Clyde S. Manuel ◽  
Anna Van Stelten ◽  
Martin Wiedmann ◽  
Kendra K. Nightingale ◽  
Renato H. Orsi
Keyword(s):  
Stop Codon ◽  
Premature Stop Codon ◽  
Phase Variation ◽  
Virulence Gene ◽  
Farm Animals ◽  
Nucleotide Substitutions ◽  
Content Type ◽  
Niche Adaptation ◽  
Frameshift Deletion ◽  
Animal Isolates

ABSTRACTInListeria monocytogenes, 18 mutations leading to premature stop codons (PMSCs) in the virulence geneinlAhave been identified to date. While most of these mutations represent nucleotide substitutions, a frameshift deletion in a 5′ seven-adenine homopolymeric tract (HT) ininlAhas also been reported. This HT may play a role in phase variation and was first identified amongL. monocytogeneslineage II ribotype DUP-1039C isolates. In order to better understand the distribution of differentinlAmutations in this ribotype, a newly developed multiplex real-time PCR assay was used to screen 368 DUP-1039C isolates from human, animal, and food-associated sources for three known 5′inlAHT alleles: (i) wild-type (WT) (A7), (ii) frameshift (FS) (A6), and (iii) guanine interruption (A2GA4) alleles. Additionally, 228 DUP-1039C isolates were screened for allinlAPMSCs; data on the presence of allinlAPMSCs for the other 140 isolates were obtained from previous studies. The statistical analysis based on 191 epidemiologically unrelated strains showed that strains withinlAPMSC mutations (n= 41) were overrepresented among food-associated isolates, while strains encoding full-length InlA (n= 150) were overrepresented among isolates from farm animals and their environments. Furthermore, the A6allele was overrepresented and the A7allele was underrepresented among food isolates, while the A6allele was underrepresented among farm and animal isolates. Our results indicate that genetic variation ininlAcontributes to niche adaptation within the lineage II subtype DUP-1039C.

scholarly journals Evaluation of Three Formulations of Culture Media for Isolation ofBrucellaspp. regarding Their Ability to Inhibit the Growth of Contaminating Organisms

2014 ◽  
Vol 2014 ◽  
pp. 1-3 ◽  
Author(s):  
Acácia F. Vicente ◽  
João M. A. P. Antunes ◽  
Gustavo H. B. Lara ◽  
Mateus S. R. Mioni ◽  
Susan D. Allendorf ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Culture Media ◽  
Agar Medium ◽  
Fungal Growth ◽  
The State ◽  
Gram Positive ◽  
Selective Media ◽  
Wild Boars ◽  
Gram Positive Bacteria ◽  
Better Than

Three culture media (Brucellaagar, Farrell medium, and CITA) were compared for their effectiveness in inhibiting contamination and for isolatingBrucellaspp. One hundred lymph nodes from pigs (n= 50) and wild boars (n= 50) with lymphadenitis were collected in slaughterhouses in the State of São Paulo and were assessed on these three selective media forBrucellaspp. All of the samples were negative forBrucellaspp. on the three culture media. On the agar medium, fungal (70 plates) and Gram-positive bacterial (59 plates) contaminants were observed; in the CITA medium, the absence of fungal and Gram-positive bacteria on 15 plates was observed; no bacterial or fungal growth was observed on the Farrell media. The results demonstrated that the CITA and Farrell media inhibited the growth of contaminants better than theBrucellaagar.

Sign in / Sign up

Export Citation Format

Share Document